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Article: Repurposing the Native Type I-F CRISPR-Cas System in Pseudomonas aeruginosa for Genome Editing
Title | Repurposing the Native Type I-F CRISPR-Cas System in Pseudomonas aeruginosa for Genome Editing |
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Authors | |
Issue Date | 2020 |
Publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home |
Citation | STAR Protocols, 2020, v. 1 n. 1, p. article no. 100039 How to Cite? |
Abstract | SUMMARY:
Repurposing the broadly distributed native CRISPR-Cas systems in prokaryotes for genome editing is emerging as a new strategy for genetic manipulations. We recently reported the establishment of a single plasmid-mediated, one-step genome-editing technique in a multidrug-resistant genotype of the opportunistic pathogen Pseudomonas aeruginosa by harnessing its endogenous type I-F CRISPR-Cas system. The platform is readily applicable in additional type I-F CRISPR-containing clinical and environmental P. aeruginosa isolates. Herein, we provide the detailed protocol for the methodology.
For complete details on the establishment and exploitation of this protocol, please refer to Xu et al. (2019). |
Persistent Identifier | http://hdl.handle.net/10722/284997 |
ISSN | 2023 Impact Factor: 1.3 2023 SCImago Journal Rankings: 0.955 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Xu, Z | - |
dc.contributor.author | LI, Y | - |
dc.contributor.author | Yan, A | - |
dc.date.accessioned | 2020-08-07T09:05:23Z | - |
dc.date.available | 2020-08-07T09:05:23Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | STAR Protocols, 2020, v. 1 n. 1, p. article no. 100039 | - |
dc.identifier.issn | 2666-1667 | - |
dc.identifier.uri | http://hdl.handle.net/10722/284997 | - |
dc.description.abstract | SUMMARY: Repurposing the broadly distributed native CRISPR-Cas systems in prokaryotes for genome editing is emerging as a new strategy for genetic manipulations. We recently reported the establishment of a single plasmid-mediated, one-step genome-editing technique in a multidrug-resistant genotype of the opportunistic pathogen Pseudomonas aeruginosa by harnessing its endogenous type I-F CRISPR-Cas system. The platform is readily applicable in additional type I-F CRISPR-containing clinical and environmental P. aeruginosa isolates. Herein, we provide the detailed protocol for the methodology. For complete details on the establishment and exploitation of this protocol, please refer to Xu et al. (2019). | - |
dc.language | eng | - |
dc.publisher | Elsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home | - |
dc.relation.ispartof | STAR Protocols | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Repurposing the Native Type I-F CRISPR-Cas System in Pseudomonas aeruginosa for Genome Editing | - |
dc.type | Article | - |
dc.identifier.email | Xu, Z: zelingxu@connect.hku.hk | - |
dc.identifier.email | Yan, A: ayan8@hku.hk | - |
dc.identifier.authority | Yan, A=rp00823 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.xpro.2020.100039 | - |
dc.identifier.scopus | eid_2-s2.0-85087305292 | - |
dc.identifier.hkuros | 312522 | - |
dc.identifier.volume | 1 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 100039 | - |
dc.identifier.epage | article no. 100039 | - |
dc.identifier.isi | WOS:001050034000003 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 2666-1667 | - |