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- Publisher Website: 10.1128/JVI.01543-09
- Scopus: eid_2-s2.0-77649219630
- PMID: 20015983
- WOS: WOS:000274330300024
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Article: Induction of protective immunity against murine gammaherpesvirus 68 infection in the absence of viral latency
Title | Induction of protective immunity against murine gammaherpesvirus 68 infection in the absence of viral latency |
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Authors | |
Issue Date | 2010 |
Citation | Journal of Virology, 2010, v. 84, n. 5, p. 2453-2465 How to Cite? |
Abstract | Human gammaherpesviruses, Epstein-Barr virus, and human herpesvirus 8/Kaposi's sarcoma-associated herpesvirus are important pathogens associated with diseases, including lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) is used as an experimental model system to study the host immune control of infection and explore novel vaccine strategies based on latency-deficient live viruses. We studied the properties and the potential of a recombinant MHV-68 (AC-RTA) in which the genes required for persistent infection were replaced by a constitutively expressed viral transcription activator, RTA, which dictates the virus to lytic replication. After intranasal infection of mice, replication of AC-RTA in the lung was attenuated, and no AC-RTA virus or viral DNA was detected in the isolated splenocytes, indicating a lack of latency in the spleen. Infection of the AC-RTA virus elicited both cellular immune responses and virus-specific IgG at a level comparable to that elicited by infection of the wild-type virus. Importantly, vaccination of AC-RTA was able to protect mice against subsequent challenge by the wild-type MHV-68. AC-RTA provides a vaccine strategy for preventing infection of human gammaherpesviruses. Furthermore, our results suggest that immunity to the major latent antigens is not required for protection. Copyright © 2010, American Society for Microbiology. All Rights Reserved. |
Persistent Identifier | http://hdl.handle.net/10722/285666 |
ISSN | 2021 Impact Factor: 6.549 2020 SCImago Journal Rankings: 2.617 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Jia, Qingmei | - |
dc.contributor.author | Freeman, Michael L. | - |
dc.contributor.author | Yager, Eric J. | - |
dc.contributor.author | McHardy, Ian | - |
dc.contributor.author | Tong, Leming | - |
dc.contributor.author | Martinez-Guzman, Dee Ann | - |
dc.contributor.author | Rickabaugh, Tammy | - |
dc.contributor.author | Hwang, Seungmin | - |
dc.contributor.author | Blackman, Marcia A. | - |
dc.contributor.author | Sun, Ren | - |
dc.contributor.author | Wu, Ting Ting | - |
dc.date.accessioned | 2020-08-18T04:56:20Z | - |
dc.date.available | 2020-08-18T04:56:20Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Journal of Virology, 2010, v. 84, n. 5, p. 2453-2465 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | http://hdl.handle.net/10722/285666 | - |
dc.description.abstract | Human gammaherpesviruses, Epstein-Barr virus, and human herpesvirus 8/Kaposi's sarcoma-associated herpesvirus are important pathogens associated with diseases, including lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) is used as an experimental model system to study the host immune control of infection and explore novel vaccine strategies based on latency-deficient live viruses. We studied the properties and the potential of a recombinant MHV-68 (AC-RTA) in which the genes required for persistent infection were replaced by a constitutively expressed viral transcription activator, RTA, which dictates the virus to lytic replication. After intranasal infection of mice, replication of AC-RTA in the lung was attenuated, and no AC-RTA virus or viral DNA was detected in the isolated splenocytes, indicating a lack of latency in the spleen. Infection of the AC-RTA virus elicited both cellular immune responses and virus-specific IgG at a level comparable to that elicited by infection of the wild-type virus. Importantly, vaccination of AC-RTA was able to protect mice against subsequent challenge by the wild-type MHV-68. AC-RTA provides a vaccine strategy for preventing infection of human gammaherpesviruses. Furthermore, our results suggest that immunity to the major latent antigens is not required for protection. Copyright © 2010, American Society for Microbiology. All Rights Reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Virology | - |
dc.title | Induction of protective immunity against murine gammaherpesvirus 68 infection in the absence of viral latency | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1128/JVI.01543-09 | - |
dc.identifier.pmid | 20015983 | - |
dc.identifier.pmcid | PMC2820913 | - |
dc.identifier.scopus | eid_2-s2.0-77649219630 | - |
dc.identifier.volume | 84 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 2453 | - |
dc.identifier.epage | 2465 | - |
dc.identifier.isi | WOS:000274330300024 | - |
dc.identifier.issnl | 0022-538X | - |