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Article: ORF18 is a transfactor that is essential for late gene transcription of a gammaherpesvirus

TitleORF18 is a transfactor that is essential for late gene transcription of a gammaherpesvirus
Authors
Issue Date2006
Citation
Journal of Virology, 2006, v. 80, n. 19, p. 9730-9740 How to Cite?
AbstractLytic replication of the tumor-associated human gammaherpesviruses Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus has important implications in pathogenesis and tumorigenesis. Herpesvirus lytic genes have been temporally classified as exhibiting immediate-early (IE), early, and late expression kinetics. Though the regulation of IE and early gene expression has been studied extensively, very little is known regarding the regulation of late gene expression. Late genes, which primarily encode virion structural proteins, require viral DNA replication for their expression. We have identified a murine gammaherpesvirus 68 (MHV-68) early lytic gene, ORF18, essential for viral replication. ORF18 is conserved in both beta- and gammaherpesviruses. By generating an MHV-68 ORF18-null virus, we characterized the stage of the virus lytic cascade that requires the function of ORF18. Gene expression profiling and quantitation of viral DNA synthesis of the ORF18-null virus revealed that the expression of early genes and viral DNA replication were not affected; however, the transcription of late genes was abolished. Hence, we have identified a gammaherpesvirus-encoded factor essential for the expression of late genes independently of viral DNA synthesis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/285905
ISSN
2019 Impact Factor: 4.501
2015 SCImago Journal Rankings: 3.347
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorArumugaswami, Vaithilingaraja-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorMartinez-Guzman, Dee Ann-
dc.contributor.authorJia, Qingmei-
dc.contributor.authorDeng, Hongyu-
dc.contributor.authorReyes, Nichole-
dc.contributor.authorSun, Ren-
dc.date.accessioned2020-08-18T04:56:57Z-
dc.date.available2020-08-18T04:56:57Z-
dc.date.issued2006-
dc.identifier.citationJournal of Virology, 2006, v. 80, n. 19, p. 9730-9740-
dc.identifier.issn0022-538X-
dc.identifier.urihttp://hdl.handle.net/10722/285905-
dc.description.abstractLytic replication of the tumor-associated human gammaherpesviruses Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus has important implications in pathogenesis and tumorigenesis. Herpesvirus lytic genes have been temporally classified as exhibiting immediate-early (IE), early, and late expression kinetics. Though the regulation of IE and early gene expression has been studied extensively, very little is known regarding the regulation of late gene expression. Late genes, which primarily encode virion structural proteins, require viral DNA replication for their expression. We have identified a murine gammaherpesvirus 68 (MHV-68) early lytic gene, ORF18, essential for viral replication. ORF18 is conserved in both beta- and gammaherpesviruses. By generating an MHV-68 ORF18-null virus, we characterized the stage of the virus lytic cascade that requires the function of ORF18. Gene expression profiling and quantitation of viral DNA synthesis of the ORF18-null virus revealed that the expression of early genes and viral DNA replication were not affected; however, the transcription of late genes was abolished. Hence, we have identified a gammaherpesvirus-encoded factor essential for the expression of late genes independently of viral DNA synthesis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.-
dc.languageeng-
dc.relation.ispartofJournal of Virology-
dc.titleORF18 is a transfactor that is essential for late gene transcription of a gammaherpesvirus-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.00246-06-
dc.identifier.pmid16973577-
dc.identifier.pmcidPMC1617240-
dc.identifier.scopuseid_2-s2.0-33748934829-
dc.identifier.volume80-
dc.identifier.issue19-
dc.identifier.spage9730-
dc.identifier.epage9740-

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