File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3390/ijms21134658
- Scopus: eid_2-s2.0-85087443701
- PMID: 32629957
- WOS: WOS:000550208700001
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: The Effect of Lycium barbarum Polysaccharides on Pyroptosis-Associated Amyloid β1-40 Oligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage
Title | The Effect of Lycium barbarum Polysaccharides on Pyroptosis-Associated Amyloid β1-40 Oligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage |
---|---|
Authors | |
Keywords | cell death drusen eye disease retina traditional Chinese medicine (TCM) |
Issue Date | 2020 |
Publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms |
Citation | International Journal of Molecular Sciences, 2020, v. 21 n. 13, p. article no. 4658 How to Cite? |
Abstract | Age-related macular degeneration (AMD) is a sight-threatening disease with limited treatment options. We investigated whether amyloid β1-40 (Aβ1-40) could cause pyroptosis and evaluated the effects of Lycium barbarum polysaccharides (LBP) on Aβ1-40 oligomers-induced retinal pigment epithelium 19 (ARPE-19) damage, which is an in vitro AMD model. Aβ1-40 oligomers verified by Western blot were added to ARPE-19 cells with or without 24 h LBP treatment. Aβ1-40 oligomers significantly decreased ARPE-19 cell viability with obvious morphological changes under light microscopy. SEM revealed swollen cells with a bubbling appearance and ruptured cell membrane, which are morphological characteristics of pyroptosis. ELISA results showed increased expression of IL-1β and IL-18, which are the final products of pyroptosis. LBP administration for 24 h had no toxic effects on ARPE-19 cells and improved cell viability and morphology while disrupting Aβ1-40 oligomerization in a dose-dependent manner. Furthermore, Aβ1-40 oligomers up-regulated the cellular immunoreactivity of pyroptosis markers including NOD-like receptors protein 3 (NLRP3), caspase-1, and membrane N-terminal cleavage product of GSDMD (GSDMD-N), which could be reversed by LBP treatment. Taken together, this study showed that LBP effectively protects the Aβ1-40 oligomers-induced pyroptotic ARPE-19 cell damages by its anti-Aβ1-40 oligomerization properties and its anti-pyroptotic effects. |
Persistent Identifier | http://hdl.handle.net/10722/287608 |
ISSN | 2023 Impact Factor: 4.9 2023 SCImago Journal Rankings: 1.179 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | YANG, M | - |
dc.contributor.author | So, KF | - |
dc.contributor.author | Lo, ACY | - |
dc.contributor.author | Lam, WC | - |
dc.date.accessioned | 2020-10-05T12:00:35Z | - |
dc.date.available | 2020-10-05T12:00:35Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | International Journal of Molecular Sciences, 2020, v. 21 n. 13, p. article no. 4658 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/10722/287608 | - |
dc.description.abstract | Age-related macular degeneration (AMD) is a sight-threatening disease with limited treatment options. We investigated whether amyloid β1-40 (Aβ1-40) could cause pyroptosis and evaluated the effects of Lycium barbarum polysaccharides (LBP) on Aβ1-40 oligomers-induced retinal pigment epithelium 19 (ARPE-19) damage, which is an in vitro AMD model. Aβ1-40 oligomers verified by Western blot were added to ARPE-19 cells with or without 24 h LBP treatment. Aβ1-40 oligomers significantly decreased ARPE-19 cell viability with obvious morphological changes under light microscopy. SEM revealed swollen cells with a bubbling appearance and ruptured cell membrane, which are morphological characteristics of pyroptosis. ELISA results showed increased expression of IL-1β and IL-18, which are the final products of pyroptosis. LBP administration for 24 h had no toxic effects on ARPE-19 cells and improved cell viability and morphology while disrupting Aβ1-40 oligomerization in a dose-dependent manner. Furthermore, Aβ1-40 oligomers up-regulated the cellular immunoreactivity of pyroptosis markers including NOD-like receptors protein 3 (NLRP3), caspase-1, and membrane N-terminal cleavage product of GSDMD (GSDMD-N), which could be reversed by LBP treatment. Taken together, this study showed that LBP effectively protects the Aβ1-40 oligomers-induced pyroptotic ARPE-19 cell damages by its anti-Aβ1-40 oligomerization properties and its anti-pyroptotic effects. | - |
dc.language | eng | - |
dc.publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms | - |
dc.relation.ispartof | International Journal of Molecular Sciences | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | cell death | - |
dc.subject | drusen | - |
dc.subject | eye disease | - |
dc.subject | retina | - |
dc.subject | traditional Chinese medicine (TCM) | - |
dc.title | The Effect of Lycium barbarum Polysaccharides on Pyroptosis-Associated Amyloid β1-40 Oligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage | - |
dc.type | Article | - |
dc.identifier.email | So, KF: hrmaskf@hku.hk | - |
dc.identifier.email | Lo, ACY: amylo@hku.hk | - |
dc.identifier.email | Lam, WC: waichlam@hku.hk | - |
dc.identifier.authority | So, KF=rp00329 | - |
dc.identifier.authority | Lo, ACY=rp00425 | - |
dc.identifier.authority | Lam, WC=rp02162 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/ijms21134658 | - |
dc.identifier.pmid | 32629957 | - |
dc.identifier.pmcid | PMC7369740 | - |
dc.identifier.scopus | eid_2-s2.0-85087443701 | - |
dc.identifier.hkuros | 314733 | - |
dc.identifier.volume | 21 | - |
dc.identifier.issue | 13 | - |
dc.identifier.spage | article no. 4658 | - |
dc.identifier.epage | article no. 4658 | - |
dc.identifier.isi | WOS:000550208700001 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 1422-0067 | - |