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Article: Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors

TitleUsage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors
Authors
Yu, YLiu, P
KeywordsscRNA-seq
Innate lymphoid cells
Cellular heterogeneity
Immune cell development
PD-1
Issue Date2019
PublisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651
Citation
Methods in Molecular Biology, 2019, v. 1884, p. 73-86 How to Cite?
DOI: http://dx.doi.org/10.1007/978-1-4939-8885-3_5
AbstractSingle-cell RNA sequencing (scRNA-seq) is a powerful tool to study immune cells, which enables an unbiased way to discover novel cell populations, biological meaningful cellular heterogeneity, and cell lineage development trajectories. Advances in scRNA-seq technologies and computational data analysis have driven a revolution in our understanding of the immune system in health and disease. Technically, the key step for scRNA-seq analysis is making a high-quality cDNA library for sequencing. Here, we describe a plate-based protocol to prepare single-cell cDNA library of bone marrow innate lymphoid precursors for next generation sequencing-based transcriptome analysis.
DescriptionCover title - Cancer Immunosurveillance: methods and protocols
Persistent Identifierhttp://hdl.handle.net/10722/288407
ISBN
9781493988853
ISSN
1064-3745
2020 SCImago Journal Rankings: 0.711

 

DC FieldValueLanguage
dc.contributor.authorYu, Y-
dc.contributor.authorLiu, P-
dc.date.accessioned2020-10-05T12:12:26Z-
dc.date.available2020-10-05T12:12:26Z-
dc.date.issued2019-
dc.identifier.citationMethods in Molecular Biology, 2019, v. 1884, p. 73-86-
dc.identifier.isbn9781493988853-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/288407-
dc.descriptionCover title - Cancer Immunosurveillance: methods and protocols-
dc.description.abstractSingle-cell RNA sequencing (scRNA-seq) is a powerful tool to study immune cells, which enables an unbiased way to discover novel cell populations, biological meaningful cellular heterogeneity, and cell lineage development trajectories. Advances in scRNA-seq technologies and computational data analysis have driven a revolution in our understanding of the immune system in health and disease. Technically, the key step for scRNA-seq analysis is making a high-quality cDNA library for sequencing. Here, we describe a plate-based protocol to prepare single-cell cDNA library of bone marrow innate lymphoid precursors for next generation sequencing-based transcriptome analysis.-
dc.languageeng-
dc.publisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI]-
dc.subjectscRNA-seq-
dc.subjectInnate lymphoid cells-
dc.subjectCellular heterogeneity-
dc.subjectImmune cell development-
dc.subjectPD-1-
dc.titleUsage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors-
dc.typeArticle-
dc.identifier.emailLiu, P: pliu88@hku.hk-
dc.identifier.authorityLiu, P=rp02328-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-4939-8885-3_5-
dc.identifier.pmid30465196-
dc.identifier.scopuseid_2-s2.0-85056947717-
dc.identifier.hkuros315694-
dc.identifier.volume1884-
dc.identifier.spage73-
dc.identifier.epage86-
dc.identifier.eissn1940-6029-
dc.publisher.placeUnited States-
dc.identifier.issnl1064-3745-

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