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- Publisher Website: 10.1002/smll.201904469
- Scopus: eid_2-s2.0-85077853419
- PMID: 31899592
- WOS: WOS:000505312100001
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Article: Droplet and Microchamber‐Based Digital Loop‐Mediated Isothermal Amplification (dLAMP)
Title | Droplet and Microchamber‐Based Digital Loop‐Mediated Isothermal Amplification (dLAMP) |
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Authors | |
Keywords | compartmentalization digital loop‐mediated isothermal amplification microfluidics pathogen detection |
Issue Date | 2020 |
Publisher | Wiley - VCH Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jabout/107640323/2421_info.html |
Citation | Small, 2020, v. 16 n. 9, p. 1904469 How to Cite? |
Abstract | Digital loop‐mediated isothermal amplification (dLAMP) refers to compartmentalizing nucleic acids and LAMP reagents into a large number of individual partitions, such as microchambers and droplets. This compartmentalization enables dLAMP to be an excellent platform to quantify the absolute number of the target nucleic acids. Owing to its low requirement for instrumentation complexity, high specificity, and strong tolerance to inhibitors in the nucleic acid samples, dLAMP has been recognized as a simple and accurate technique to quantify pathogenic nucleic acid. Herein, the general process of dLAMP techniques is summarized, the current dLAMP techniques are categorized, and a comprehensive discussion on different types of dLAMP techniques is presented. Also, the challenges of the current dLAMP are illustrated together with the possible strategies to address these challenges. In the end, the future directions of the dLAMP developments, including multitarget detection, multisample detection, and processing nucleic acid extraction are outlined. With recently significant advances in dLAMP, this technology has the potential to see more widespread use beyond the laboratory in the future. |
Persistent Identifier | http://hdl.handle.net/10722/289369 |
ISSN | 2023 Impact Factor: 13.0 2023 SCImago Journal Rankings: 3.348 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | YUAN, H | - |
dc.contributor.author | CHAO, Y | - |
dc.contributor.author | Shum, HC | - |
dc.date.accessioned | 2020-10-22T08:11:40Z | - |
dc.date.available | 2020-10-22T08:11:40Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Small, 2020, v. 16 n. 9, p. 1904469 | - |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.uri | http://hdl.handle.net/10722/289369 | - |
dc.description.abstract | Digital loop‐mediated isothermal amplification (dLAMP) refers to compartmentalizing nucleic acids and LAMP reagents into a large number of individual partitions, such as microchambers and droplets. This compartmentalization enables dLAMP to be an excellent platform to quantify the absolute number of the target nucleic acids. Owing to its low requirement for instrumentation complexity, high specificity, and strong tolerance to inhibitors in the nucleic acid samples, dLAMP has been recognized as a simple and accurate technique to quantify pathogenic nucleic acid. Herein, the general process of dLAMP techniques is summarized, the current dLAMP techniques are categorized, and a comprehensive discussion on different types of dLAMP techniques is presented. Also, the challenges of the current dLAMP are illustrated together with the possible strategies to address these challenges. In the end, the future directions of the dLAMP developments, including multitarget detection, multisample detection, and processing nucleic acid extraction are outlined. With recently significant advances in dLAMP, this technology has the potential to see more widespread use beyond the laboratory in the future. | - |
dc.language | eng | - |
dc.publisher | Wiley - VCH Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jabout/107640323/2421_info.html | - |
dc.relation.ispartof | Small | - |
dc.rights | This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | - |
dc.subject | compartmentalization | - |
dc.subject | digital loop‐mediated isothermal amplification | - |
dc.subject | microfluidics | - |
dc.subject | pathogen detection | - |
dc.title | Droplet and Microchamber‐Based Digital Loop‐Mediated Isothermal Amplification (dLAMP) | - |
dc.type | Article | - |
dc.identifier.email | Shum, HC: ashum@hku.hk | - |
dc.identifier.authority | Shum, HC=rp01439 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/smll.201904469 | - |
dc.identifier.pmid | 31899592 | - |
dc.identifier.scopus | eid_2-s2.0-85077853419 | - |
dc.identifier.hkuros | 317477 | - |
dc.identifier.volume | 16 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 1904469 | - |
dc.identifier.epage | 1904469 | - |
dc.identifier.isi | WOS:000505312100001 | - |
dc.publisher.place | Germany | - |
dc.identifier.issnl | 1613-6810 | - |