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Article: p53 mutants cooperate with HIF-1 in transcriptional regulation of extracellular matrix components to promote tumor progression

Titlep53 mutants cooperate with HIF-1 in transcriptional regulation of extracellular matrix components to promote tumor progression
Authors
KeywordsHIF
p53
Microenvironment
Chromatin architecture
SWI/SNF
Issue Date2018
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2018, v. 115, n. 46, p. E10869-E10878 How to Cite?
AbstractMutations in the TP53 gene and microenvironmentally driven activation of hypoxia-inducible factor-1 (HIF-1) typically occur in later stages of tumorigenesis. An ongoing challenge is the identification of molecular determinants of advanced cancer pathogenesis to design alternative last-line therapeutic options. Here, we report that p53 mutants influence the tumor microenvironment by cooperating with HIF-1 to promote cancer progression. We demonstrate that in non-small cell lung cancer (NSCLC), p53 mutants exert a gain-of-function (GOF) effect on HIF-1, thus regulating a selective gene expression signature involved in protumorigenic functions. Hypoxia-mediated activation of HIF-1 leads to the formation of a p53 mutant/HIF-1 complex that physically binds the SWI/SNF chromatin remodeling complex, promoting expression of a selective subset of hypoxia-responsive genes. Depletion of p53 mutants impairs the HIF-mediated up-regulation of extracellular matrix (ECM) components, including type VIIa1 collagen and laminin-γ2, thus affecting tumorigenic potential of NSCLC cells in vitro and in mouse models in vivo. Analysis of surgically resected human NSCLC revealed that expression of this ECM gene signature was highly correlated with hypoxic tumors exclusively in patients carrying p53 mutations and was associated with poor prognosis. Our data reveal a GOF effect of p53 mutants in hypoxic tumors and suggest synergistic activities of p53 and HIF-1. These findings have important implications for cancer progression and might provide innovative last-line treatment options for advanced NSCLC.
Persistent Identifierhttp://hdl.handle.net/10722/292090
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAmelio, Ivano-
dc.contributor.authorMancini, Mara-
dc.contributor.authorPetrova, Varvara-
dc.contributor.authorCairns, Rob A.-
dc.contributor.authorVikhreva, Polina-
dc.contributor.authorNicolai, Sara-
dc.contributor.authorMarini, Alberto-
dc.contributor.authorAntonov, Alexey A.-
dc.contributor.authorLe Quesne, John-
dc.contributor.authorBaena Acevedo, Juvenal D.-
dc.contributor.authorDudek, Kate-
dc.contributor.authorSozzi, Gabriella-
dc.contributor.authorPastorino, Ugo-
dc.contributor.authorKnight, Richard A.-
dc.contributor.authorMak, Tak W.-
dc.contributor.authorMelino, Gerry-
dc.date.accessioned2020-11-17T14:55:44Z-
dc.date.available2020-11-17T14:55:44Z-
dc.date.issued2018-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2018, v. 115, n. 46, p. E10869-E10878-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/292090-
dc.description.abstractMutations in the TP53 gene and microenvironmentally driven activation of hypoxia-inducible factor-1 (HIF-1) typically occur in later stages of tumorigenesis. An ongoing challenge is the identification of molecular determinants of advanced cancer pathogenesis to design alternative last-line therapeutic options. Here, we report that p53 mutants influence the tumor microenvironment by cooperating with HIF-1 to promote cancer progression. We demonstrate that in non-small cell lung cancer (NSCLC), p53 mutants exert a gain-of-function (GOF) effect on HIF-1, thus regulating a selective gene expression signature involved in protumorigenic functions. Hypoxia-mediated activation of HIF-1 leads to the formation of a p53 mutant/HIF-1 complex that physically binds the SWI/SNF chromatin remodeling complex, promoting expression of a selective subset of hypoxia-responsive genes. Depletion of p53 mutants impairs the HIF-mediated up-regulation of extracellular matrix (ECM) components, including type VIIa1 collagen and laminin-γ2, thus affecting tumorigenic potential of NSCLC cells in vitro and in mouse models in vivo. Analysis of surgically resected human NSCLC revealed that expression of this ECM gene signature was highly correlated with hypoxic tumors exclusively in patients carrying p53 mutations and was associated with poor prognosis. Our data reveal a GOF effect of p53 mutants in hypoxic tumors and suggest synergistic activities of p53 and HIF-1. These findings have important implications for cancer progression and might provide innovative last-line treatment options for advanced NSCLC.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectHIF-
dc.subjectp53-
dc.subjectMicroenvironment-
dc.subjectChromatin architecture-
dc.subjectSWI/SNF-
dc.titlep53 mutants cooperate with HIF-1 in transcriptional regulation of extracellular matrix components to promote tumor progression-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1073/pnas.1808314115-
dc.identifier.pmid30381462-
dc.identifier.pmcidPMC6243248-
dc.identifier.scopuseid_2-s2.0-85056494217-
dc.identifier.volume115-
dc.identifier.issue46-
dc.identifier.spageE10869-
dc.identifier.epageE10878-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000449934400009-
dc.identifier.issnl0027-8424-

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