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Article: Antibiotic Resistance Genes and Correlations with Microbial Community and Metal Resistance Genes in Full-Scale Biogas Reactors As Revealed by Metagenomic Analysis

TitleAntibiotic Resistance Genes and Correlations with Microbial Community and Metal Resistance Genes in Full-Scale Biogas Reactors As Revealed by Metagenomic Analysis
Authors
KeywordsAntibiotic resistance genes
Canonical Correspondence Analysis
Environmental variables
High-throughput sequencing
Hydraulic retention time
Issue Date2017
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/esthag
Citation
Environmental Science & Technology, 2017, v. 51 n. 7, p. 4069-4080 How to Cite?
AbstractDigested residues from biogas plants are often used as biofertilizers for agricultural crops cultivation. The antibiotic resistance genes (ARGs) in digested residues pose a high risk to public health due to their potential spread to the disease-causing microorganisms and thus reduce the susceptibility of disease-causing microorganisms to antibiotics in medical treatment. A high-throughput sequencing (HTS)-based metagenomic approach was used in the present study to investigate the variations of ARGs in full-scale biogas reactors and the correlations of ARGs with microbial communities and metal resistance genes (MRGs). The total abundance of ARGs in all the samples varied from 7 × 10–3 to 1.08 × 10–1 copy of ARG/copy of 16S-rRNA gene, and the samples obtained from thermophilic biogas reactors had a lower total abundance of ARGs, indicating the superiority of thermophilic anaerobic digestion for ARGs removal. ARGs in all the samples were composed of 175 ARG subtypes; however, only 7 ARG subtypes were shared by all the samples. Principal component analysis and canonical correspondence analysis clustered the samples into three groups (samples from manure-based mesophilic reactors, manure-based thermophilic reactors, and sludge-based mesophilic reactors), and substrate, temperature, and hydraulic retention time (HRT) as well as volatile fatty acids (VFAs) were identified as crucial environmental variables affecting the ARGs compositions. Procrustes analysis revealed microbial community composition was the determinant of ARGs composition in biogas reactors, and there was also a significant correlation between ARGs composition and MRGs composition. Network analysis further revealed the co-occurrence of ARGs with specific microorganisms and MRGs.
Persistent Identifierhttp://hdl.handle.net/10722/293297
ISSN
2019 Impact Factor: 7.864
2015 SCImago Journal Rankings: 2.664

 

DC FieldValueLanguage
dc.contributor.authorLuo, G-
dc.contributor.authorLi, B-
dc.contributor.authorLi, LG-
dc.contributor.authorZhang, T-
dc.contributor.authorAngelidaki, I-
dc.date.accessioned2020-11-23T08:14:43Z-
dc.date.available2020-11-23T08:14:43Z-
dc.date.issued2017-
dc.identifier.citationEnvironmental Science & Technology, 2017, v. 51 n. 7, p. 4069-4080-
dc.identifier.issn0013-936X-
dc.identifier.urihttp://hdl.handle.net/10722/293297-
dc.description.abstractDigested residues from biogas plants are often used as biofertilizers for agricultural crops cultivation. The antibiotic resistance genes (ARGs) in digested residues pose a high risk to public health due to their potential spread to the disease-causing microorganisms and thus reduce the susceptibility of disease-causing microorganisms to antibiotics in medical treatment. A high-throughput sequencing (HTS)-based metagenomic approach was used in the present study to investigate the variations of ARGs in full-scale biogas reactors and the correlations of ARGs with microbial communities and metal resistance genes (MRGs). The total abundance of ARGs in all the samples varied from 7 × 10–3 to 1.08 × 10–1 copy of ARG/copy of 16S-rRNA gene, and the samples obtained from thermophilic biogas reactors had a lower total abundance of ARGs, indicating the superiority of thermophilic anaerobic digestion for ARGs removal. ARGs in all the samples were composed of 175 ARG subtypes; however, only 7 ARG subtypes were shared by all the samples. Principal component analysis and canonical correspondence analysis clustered the samples into three groups (samples from manure-based mesophilic reactors, manure-based thermophilic reactors, and sludge-based mesophilic reactors), and substrate, temperature, and hydraulic retention time (HRT) as well as volatile fatty acids (VFAs) were identified as crucial environmental variables affecting the ARGs compositions. Procrustes analysis revealed microbial community composition was the determinant of ARGs composition in biogas reactors, and there was also a significant correlation between ARGs composition and MRGs composition. Network analysis further revealed the co-occurrence of ARGs with specific microorganisms and MRGs.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/esthag-
dc.relation.ispartofEnvironmental Science & Technology-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.subjectAntibiotic resistance genes-
dc.subjectCanonical Correspondence Analysis-
dc.subjectEnvironmental variables-
dc.subjectHigh-throughput sequencing-
dc.subjectHydraulic retention time-
dc.titleAntibiotic Resistance Genes and Correlations with Microbial Community and Metal Resistance Genes in Full-Scale Biogas Reactors As Revealed by Metagenomic Analysis-
dc.typeArticle-
dc.identifier.emailLi, LG: liliguan@hku.hk-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.authorityZhang, T=rp00211-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acs.est.6b05100-
dc.identifier.pmid28272884-
dc.identifier.scopuseid_2-s2.0-85019656562-
dc.identifier.hkuros319377-
dc.identifier.volume51-
dc.identifier.issue7-
dc.identifier.spage4069-
dc.identifier.epage4080-
dc.publisher.placeUnited States-

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