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- Publisher Website: 10.1038/ncb2988
- Scopus: eid_2-s2.0-84903701173
- PMID: 24952464
- WOS: WOS:000338187900007
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Article: The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment
Title | The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment |
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Authors | |
Issue Date | 2014 |
Citation | Nature Cell Biology, 2014, v. 16, n. 7, p. 663-672 How to Cite? |
Abstract | Mammalian Hedgehog (Hh) signal transduction requires a primary cilium, a microtubule-based organelle, and the Gli-Sufu complexes that mediate Hh signalling, which are enriched at cilia tips. Kif7, a kinesin-4 family protein, is a conserved regulator of the Hh signalling pathway and a human ciliopathy protein. Here we show that Kif7 localizes to the cilium tip, the site of microtubule plus ends, where it limits cilium length and controls cilium structure. Purified recombinant Kif7 binds the plus ends of growing microtubules in vitro, where it reduces the rate of microtubule growth and increases the frequency of microtubule catastrophe. Kif7 is not required for normal intraflagellar transport or for trafficking of Hh pathway proteins into cilia. Instead, a central function of Kif7 in the mammalian Hh pathway is to control cilium architecture and to create a single cilium tip compartment, where Gli-Sufu activity can be correctly regulated. © 2014 Macmillan Publishers Limited. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/298082 |
ISSN | 2023 Impact Factor: 17.3 2023 SCImago Journal Rankings: 8.913 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | He, Mu | - |
dc.contributor.author | Subramanian, Radhika | - |
dc.contributor.author | Bangs, Fiona | - |
dc.contributor.author | Omelchenko, Tatiana | - |
dc.contributor.author | Liem, Karel F. | - |
dc.contributor.author | Kapoor, Tarun M. | - |
dc.contributor.author | Anderson, Kathryn V. | - |
dc.date.accessioned | 2021-04-08T03:07:38Z | - |
dc.date.available | 2021-04-08T03:07:38Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Nature Cell Biology, 2014, v. 16, n. 7, p. 663-672 | - |
dc.identifier.issn | 1465-7392 | - |
dc.identifier.uri | http://hdl.handle.net/10722/298082 | - |
dc.description.abstract | Mammalian Hedgehog (Hh) signal transduction requires a primary cilium, a microtubule-based organelle, and the Gli-Sufu complexes that mediate Hh signalling, which are enriched at cilia tips. Kif7, a kinesin-4 family protein, is a conserved regulator of the Hh signalling pathway and a human ciliopathy protein. Here we show that Kif7 localizes to the cilium tip, the site of microtubule plus ends, where it limits cilium length and controls cilium structure. Purified recombinant Kif7 binds the plus ends of growing microtubules in vitro, where it reduces the rate of microtubule growth and increases the frequency of microtubule catastrophe. Kif7 is not required for normal intraflagellar transport or for trafficking of Hh pathway proteins into cilia. Instead, a central function of Kif7 in the mammalian Hh pathway is to control cilium architecture and to create a single cilium tip compartment, where Gli-Sufu activity can be correctly regulated. © 2014 Macmillan Publishers Limited. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | Nature Cell Biology | - |
dc.title | The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1038/ncb2988 | - |
dc.identifier.pmid | 24952464 | - |
dc.identifier.pmcid | PMC4085576 | - |
dc.identifier.scopus | eid_2-s2.0-84903701173 | - |
dc.identifier.volume | 16 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | 663 | - |
dc.identifier.epage | 672 | - |
dc.identifier.eissn | 1476-4679 | - |
dc.identifier.isi | WOS:000338187900007 | - |
dc.identifier.issnl | 1465-7392 | - |