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Article: Extensible combinatorial CRISPR screening in mammalian cells

TitleExtensible combinatorial CRISPR screening in mammalian cells
Authors
Zhou, PWan, YKChan, BKCChoi, GCGWong, ASL
KeywordsHigh Throughput Screening
CRISPR
Issue Date2021
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home
Citation
STAR Protocols, 2021, v. 2 n. 1, article no. 100255 How to Cite?
DOI: http://dx.doi.org/10.1016/j.xpro.2020.100255
AbstractThe CRISPR-Cas system coupled with Combinatorial Genetics En Masse (CombiGEM) enables systematic analysis of high-order genetic perturbations that are important for understanding biological processes and discovering therapeutic target combinations. Here, we present detailed steps and technical considerations for building multiplexed guide RNA libraries and carrying out a combinatorial CRISPR screen in mammalian cells. We also present an analytical pipeline, CombiPIPE, for mapping two- and three-way genetic interactions. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2020).
Persistent Identifierhttp://hdl.handle.net/10722/305727
ISSN
2666-1667
PubMed Central ID
PMC7809193

 

DC FieldValueLanguage
dc.contributor.authorZhou, P-
dc.contributor.authorWan, YK-
dc.contributor.authorChan, BKC-
dc.contributor.authorChoi, GCG-
dc.contributor.authorWong, ASL-
dc.date.accessioned2021-10-20T10:13:29Z-
dc.date.available2021-10-20T10:13:29Z-
dc.date.issued2021-
dc.identifier.citationSTAR Protocols, 2021, v. 2 n. 1, article no. 100255-
dc.identifier.issn2666-1667-
dc.identifier.urihttp://hdl.handle.net/10722/305727-
dc.description.abstractThe CRISPR-Cas system coupled with Combinatorial Genetics En Masse (CombiGEM) enables systematic analysis of high-order genetic perturbations that are important for understanding biological processes and discovering therapeutic target combinations. Here, we present detailed steps and technical considerations for building multiplexed guide RNA libraries and carrying out a combinatorial CRISPR screen in mammalian cells. We also present an analytical pipeline, CombiPIPE, for mapping two- and three-way genetic interactions. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2020).-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home-
dc.relation.ispartofSTAR Protocols-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectHigh Throughput Screening-
dc.subjectCRISPR-
dc.titleExtensible combinatorial CRISPR screening in mammalian cells-
dc.typeArticle-
dc.identifier.emailZhou, P: zhoupeng@hku.hk-
dc.identifier.emailChoi, GCG: gigichoi@hku.hk-
dc.identifier.emailWong, ASL: aslw@hku.hk-
dc.identifier.authorityWong, ASL=rp02139-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.xpro.2020.100255-
dc.identifier.pmid33490975-
dc.identifier.pmcidPMC7809193-
dc.identifier.scopuseid_2-s2.0-85122815055-
dc.identifier.hkuros327182-
dc.identifier.volume2-
dc.identifier.issue1-
dc.identifier.spagearticle no. 100255-
dc.identifier.epagearticle no. 100255-
dc.publisher.placeUnited States-

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