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Article: Overexpression of Lin28a induces a primary ovarian insufficiency phenotype via facilitation of primordial follicle activation in mice

TitleOverexpression of Lin28a induces a primary ovarian insufficiency phenotype via facilitation of primordial follicle activation in mice
Authors
KeywordsLin28a
Wnt
LRP5
mTOR
Primordial follicle activation
Issue Date2022
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce
Citation
Molecular and Cellular Endocrinology, 2022, v. 539, article no. 111460 How to Cite?
AbstractLin28a is an RNA binding protein and increasing evidence has indicated its role in regulating female fertility. Lin28a has been reported to be involved in ovarian follicle activation. However, its role and mechanisms in regulating primordial follicle activation have not yet been explored. To test whether overexpression of Lin28a activates ovarian primordial follicles, studies were conducted in wild type (WT) and Lin28a Tg mice. Female Lin28a Tg mice at 4-month old exhibited significantly smaller litter size and fewer ovulated oocytes when compared with the WT mice. By 6-month of age, these parameters in Lin28a Tg mice were less than 20% of the WT mice. At postnatal day (PD) 14, the number of primordial follicles was significantly decreased but the number of primary follicles was significantly increased in the transgenic mice. The number of primordial follicles, secondary and antral follicles in these mice were drastically reduced at PD21. In the ovary of Lin28a Tg mice, there were activation of Wnt/β-catenin signaling and its downstream mTOR pathway. Interestingly, overexpression of Lin28a, which can also act as transcriptional activator, activated Wnt signaling through enhancing the transcription of Wnt co-receptor LRP5. In conclusion, overexpression of Lin28a induced a primary ovarian insufficiency phenotype in long term via facilitating Wnt/β-catenin signaling leading to activation of primordial follicles.
Persistent Identifierhttp://hdl.handle.net/10722/307752
ISSN
2021 Impact Factor: 4.369
2020 SCImago Journal Rankings: 1.296
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, J-
dc.contributor.authorLiu, W-
dc.contributor.authorLee, KF-
dc.contributor.authorLiu, K-
dc.contributor.authorWong, BPC-
dc.contributor.authorYeung, WSB-
dc.date.accessioned2021-11-12T13:37:18Z-
dc.date.available2021-11-12T13:37:18Z-
dc.date.issued2022-
dc.identifier.citationMolecular and Cellular Endocrinology, 2022, v. 539, article no. 111460-
dc.identifier.issn0303-7207-
dc.identifier.urihttp://hdl.handle.net/10722/307752-
dc.description.abstractLin28a is an RNA binding protein and increasing evidence has indicated its role in regulating female fertility. Lin28a has been reported to be involved in ovarian follicle activation. However, its role and mechanisms in regulating primordial follicle activation have not yet been explored. To test whether overexpression of Lin28a activates ovarian primordial follicles, studies were conducted in wild type (WT) and Lin28a Tg mice. Female Lin28a Tg mice at 4-month old exhibited significantly smaller litter size and fewer ovulated oocytes when compared with the WT mice. By 6-month of age, these parameters in Lin28a Tg mice were less than 20% of the WT mice. At postnatal day (PD) 14, the number of primordial follicles was significantly decreased but the number of primary follicles was significantly increased in the transgenic mice. The number of primordial follicles, secondary and antral follicles in these mice were drastically reduced at PD21. In the ovary of Lin28a Tg mice, there were activation of Wnt/β-catenin signaling and its downstream mTOR pathway. Interestingly, overexpression of Lin28a, which can also act as transcriptional activator, activated Wnt signaling through enhancing the transcription of Wnt co-receptor LRP5. In conclusion, overexpression of Lin28a induced a primary ovarian insufficiency phenotype in long term via facilitating Wnt/β-catenin signaling leading to activation of primordial follicles.-
dc.languageeng-
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce-
dc.relation.ispartofMolecular and Cellular Endocrinology-
dc.subjectLin28a-
dc.subjectWnt-
dc.subjectLRP5-
dc.subjectmTOR-
dc.subjectPrimordial follicle activation-
dc.titleOverexpression of Lin28a induces a primary ovarian insufficiency phenotype via facilitation of primordial follicle activation in mice-
dc.typeArticle-
dc.identifier.emailLiu, W: liuwm@hkucc.hku.hk-
dc.identifier.emailLee, KF: ckflee@hku.hk-
dc.identifier.emailLiu, K: kliugc@hku.hk-
dc.identifier.emailYeung, WSB: wsbyeung@hku.hk-
dc.identifier.authorityLee, KF=rp00458-
dc.identifier.authorityLiu, K=rp02475-
dc.identifier.authorityYeung, WSB=rp00331-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mce.2021.111460-
dc.identifier.pmid34543700-
dc.identifier.scopuseid_2-s2.0-85116440754-
dc.identifier.hkuros329649-
dc.identifier.volume539-
dc.identifier.spagearticle no. 111460-
dc.identifier.epagearticle no. 111460-
dc.identifier.isiWOS:000708861200008-
dc.publisher.placeIreland-

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