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Conference Paper: Systematic screening of three-way combinatorial druggable targets for cancer therapy

TitleSystematic screening of three-way combinatorial druggable targets for cancer therapy
Authors
Zhou, PChan, BKCWan, YKYUEN, CTLChoi, GCGLi, XTong, CSWWong, SL
KeywordsCRISPR screen
druggable targets
multiplexed genome editing
precision therapy
Issue Date2021
PublisherNational Cancer Institute.
Citation
Cell-NCI Symposium: Beyond Cancer Genomics Toward Precision Oncology (CBCG2021) (Online), October 4-6, 2021 How to Cite?
AbstractWe present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying three-way synergistic therapeutic target combinations and successfully validate triple-combination regimens for suppression of cancer cell growth. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets for precision oncology.
DescriptionPoster Session 2, Wednesday, 6th October, 2021, P2.31
Persistent Identifierhttp://hdl.handle.net/10722/314764

 

DC FieldValueLanguage
dc.contributor.authorZhou, P-
dc.contributor.authorChan, BKC-
dc.contributor.authorWan, YK-
dc.contributor.authorYUEN, CTL-
dc.contributor.authorChoi, GCG-
dc.contributor.authorLi, X-
dc.contributor.authorTong, CSW-
dc.contributor.authorWong, SL-
dc.date.accessioned2022-08-05T09:34:09Z-
dc.date.available2022-08-05T09:34:09Z-
dc.date.issued2021-
dc.identifier.citationCell-NCI Symposium: Beyond Cancer Genomics Toward Precision Oncology (CBCG2021) (Online), October 4-6, 2021-
dc.identifier.urihttp://hdl.handle.net/10722/314764-
dc.descriptionPoster Session 2, Wednesday, 6th October, 2021, P2.31-
dc.description.abstractWe present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying three-way synergistic therapeutic target combinations and successfully validate triple-combination regimens for suppression of cancer cell growth. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets for precision oncology.-
dc.languageeng-
dc.publisherNational Cancer Institute. -
dc.subjectCRISPR screen-
dc.subjectdruggable targets-
dc.subjectmultiplexed genome editing-
dc.subjectprecision therapy-
dc.titleSystematic screening of three-way combinatorial druggable targets for cancer therapy-
dc.typeConference_Paper-
dc.identifier.emailZhou, P: zhoupeng@hku.hk-
dc.identifier.emailChan, BKC: beckyckc@hku.hk-
dc.identifier.emailChoi, GCG: gigichoi@hku.hk-
dc.identifier.emailTong, CSW: cswtong@hku.hk-
dc.identifier.emailWong, SL: aslw@hku.hk-
dc.identifier.authorityWong, SL=rp02139-
dc.identifier.hkuros334844-
dc.publisher.placeUnited States-

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