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- Publisher Website: 10.1016/S1872-2040(09)60017-7
- Scopus: eid_2-s2.0-77954605617
- WOS: WOS:000274371500017
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Article: Peptide Labeling with Improved 18O Incorporation Method
| Title | Peptide Labeling with Improved <sup>18</sup>O Incorporation Method |
|---|---|
| Authors | |
| Keywords | 18 O stable isotope labeling Peptide Proteome |
| Issue Date | 2010 |
| Citation | Chinese Journal of Analytical Chemistry, 2010, v. 38, n. 1, p. 91-94 How to Cite? |
| Abstract | To optimize the 18O-labeling method, two key aspects, peptide dispersion and trypsin deactivation, were discussed. The addition of RapiGest™ SF in H218O and microwave heating enhanced labeling efficiency of α-casein digested peptides (18O/16O ratio > 99%). Chemical modification with tris (2-carboxyethyl) phosphine and iodoacetamide resulted in trypsin deactivation completely. No significant back-exchange from 18O to 16O was observed after labeled in 6 days. The experiment result with peptide mixture from thyroglobulin showed that the improved method could be effectively used to label protein and peptide. |
| Persistent Identifier | http://hdl.handle.net/10722/342376 |
| ISI Accession Number ID |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | ZHAO, Yan | - |
| dc.contributor.author | LU, Zhuang | - |
| dc.contributor.author | JIA, Wei | - |
| dc.contributor.author | YING, Wan Tao | - |
| dc.contributor.author | QIAN, Xiao Hong | - |
| dc.date.accessioned | 2024-04-17T07:03:23Z | - |
| dc.date.available | 2024-04-17T07:03:23Z | - |
| dc.date.issued | 2010 | - |
| dc.identifier.citation | Chinese Journal of Analytical Chemistry, 2010, v. 38, n. 1, p. 91-94 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/342376 | - |
| dc.description.abstract | To optimize the 18O-labeling method, two key aspects, peptide dispersion and trypsin deactivation, were discussed. The addition of RapiGest™ SF in H218O and microwave heating enhanced labeling efficiency of α-casein digested peptides (18O/16O ratio > 99%). Chemical modification with tris (2-carboxyethyl) phosphine and iodoacetamide resulted in trypsin deactivation completely. No significant back-exchange from 18O to 16O was observed after labeled in 6 days. The experiment result with peptide mixture from thyroglobulin showed that the improved method could be effectively used to label protein and peptide. | - |
| dc.language | eng | - |
| dc.relation.ispartof | Chinese Journal of Analytical Chemistry | - |
| dc.subject | 18 O stable isotope labeling | - |
| dc.subject | Peptide | - |
| dc.subject | Proteome | - |
| dc.title | Peptide Labeling with Improved <sup>18</sup>O Incorporation Method | - |
| dc.type | Article | - |
| dc.description.nature | link_to_subscribed_fulltext | - |
| dc.identifier.doi | 10.1016/S1872-2040(09)60017-7 | - |
| dc.identifier.scopus | eid_2-s2.0-77954605617 | - |
| dc.identifier.volume | 38 | - |
| dc.identifier.issue | 1 | - |
| dc.identifier.spage | 91 | - |
| dc.identifier.epage | 94 | - |
| dc.identifier.eissn | 1872-2040 | - |
| dc.identifier.isi | WOS:000274371500017 | - |