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Conference Paper: Identification and characterization of stilbene derivatives in infected sorghum seedlings

TitleIdentification and characterization of stilbene derivatives in infected sorghum seedlings
Authors
KeywordsBiology
Botany
Issue Date2007
PublisherAmerican Phytopathological Society. The Journal's web site is located at http://www.aspnet.org/phyto
Citation
2007 APS SON Joint Meeting, San Diego, California, USA, 28 July - 1 August 2007. In Phytopathology, 2007, v. 97 n. 7 Suppl, p. S67 How to Cite?
AbstractWe have previously identified a pathogen-inducible stilbene synthase gene (SbSTS1) in sorghum. In vitro enzyme assays of recombinant SbSTS1 demonstrated that trans-resveratrol was a reaction product when p-coumaroylCoA was used as a substrate. In transgenic Arabidopsis plants over-expressing SbSTS1, cis-piceid was found as the major stilbene metabolite. Here we use a combination of mass spectrometric methods (precursor ion scan, product ion scan, multiple reaction monitoring) to identify stilbene derivatives in sorghum seedlings following inoculation with the anthracnose pathogen Colletotrichum graminicola. Results indicated that trans-piceid accumulates predominantly in infected seedlings, together with a resveratrol acetyl-hexoside. Interestingly trans-piceid accumulation was considerably higher in a sorghum cultivar that does not produce the 3-deoxyanthocyanidin phytoalexins. In vitro bioassays showed that trans-piceid is less toxic than trans-resveratrol toward C. graminicola. The role of trans-piceid accumulation in sorghum disease resistance will be discussed.
Persistent Identifierhttp://hdl.handle.net/10722/57138
ISSN
2023 Impact Factor: 2.6
2023 SCImago Journal Rankings: 0.783

 

DC FieldValueLanguage
dc.contributor.authorLo, Cen_HK
dc.contributor.authorYu, Ken_HK
dc.date.accessioned2010-04-12T01:26:55Z-
dc.date.available2010-04-12T01:26:55Z-
dc.date.issued2007en_HK
dc.identifier.citation2007 APS SON Joint Meeting, San Diego, California, USA, 28 July - 1 August 2007. In Phytopathology, 2007, v. 97 n. 7 Suppl, p. S67en_HK
dc.identifier.issn0031-949Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/57138-
dc.description.abstractWe have previously identified a pathogen-inducible stilbene synthase gene (SbSTS1) in sorghum. In vitro enzyme assays of recombinant SbSTS1 demonstrated that trans-resveratrol was a reaction product when p-coumaroylCoA was used as a substrate. In transgenic Arabidopsis plants over-expressing SbSTS1, cis-piceid was found as the major stilbene metabolite. Here we use a combination of mass spectrometric methods (precursor ion scan, product ion scan, multiple reaction monitoring) to identify stilbene derivatives in sorghum seedlings following inoculation with the anthracnose pathogen Colletotrichum graminicola. Results indicated that trans-piceid accumulates predominantly in infected seedlings, together with a resveratrol acetyl-hexoside. Interestingly trans-piceid accumulation was considerably higher in a sorghum cultivar that does not produce the 3-deoxyanthocyanidin phytoalexins. In vitro bioassays showed that trans-piceid is less toxic than trans-resveratrol toward C. graminicola. The role of trans-piceid accumulation in sorghum disease resistance will be discussed.-
dc.languageengen_HK
dc.publisherAmerican Phytopathological Society. The Journal's web site is located at http://www.aspnet.org/phytoen_HK
dc.relation.ispartofPhytopathology-
dc.subjectBiologyen_HK
dc.subjectBotanyen_HK
dc.titleIdentification and characterization of stilbene derivatives in infected sorghum seedlingsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.emailYu, K: christinekyyu@hotmail.comen_HK
dc.description.natureabstracten_HK
dc.identifier.pmid18943563en_HK
dc.identifier.hkuros139137-
dc.identifier.volume97-
dc.identifier.issue7 Suppl-
dc.identifier.spageS67-
dc.identifier.epageS67-
dc.identifier.partofdoi10.1094/PHYTO.2007.97.7.S1-
dc.identifier.issnl0031-949X-

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