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Article: Comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord, and placenta: Implication in the migration

TitleComparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord, and placenta: Implication in the migration
Authors
Li, GZhang, XAWang, HWang, XMeng, CLChan, CYYew, DTWTsang, KSLi, KTsai, SNNgai, SMHan, ZCLin, MCMHe, MLKung, HF
KeywordsBone marrow
Mesenchymal stem cells
Migration
Placenta
Umbilical cord
Issue Date2009
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2009, v. 9 n. 1, p. 20-30 How to Cite?
DOI: http://dx.doi.org/10.1002/pmic.200701195
AbstractUmbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) as sources of mesenchymal stem cells (MSC) for cell therapy, with both UC- and P-MSC possess immunophenotypic and functional characteristics similar to BM-MSC. However, their migration capacity, which is indispensable during tissue regeneration process, is unclear. Under defined conditions, the migration capacity of BM- and P-MSC was found 5.9- and 3.2-folds higher than that of UC-MSC, respectively. By the use of 2-DE and combined MS and MS/MS analysis, six differentially expressed proteins were identified among these MSC samples, with five of them known to be involved in cell migration as migration enhancing or inhibiting proteins. Consistent with their migration capacity, the levels ofmigration enhancing proteins including cathepsin B, cathepsin D and prohibitin,were significantly lower in UC-MSC when compared with those in BM- and P-MSC. For the migration inhibiting proteins such as plasminogen activator inhibitor-1 (PAI-1) and manganese superoxide dismutase, higher expression was found in the UC-MSC. We also showed that the overexpression of the PAI-1 impaired the migration capacity of BM- and P- MSC while silencing of PAI-1 enhanced the migration capacity of UC-MSC. Our study indicates that PAI-1 and other migration-related proteins are pivotal in governing the migration capacity of MSC. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/58446
ISSN
1615-9853
2021 Impact Factor: 5.393
2020 SCImago Journal Rankings: 1.260
ISI Accession Number ID
WOS:000262568500003
Funding AgencyGrant Number
Li Ka Shing Institute of Health SciencesCUHK 7422_03M
Funding Information:

The authors would like to thank Dr. Didier Tronofor kindly providing the lentiviral vector pLVTHM and its package plasmids. We also would like to thank the donors of bone marrow, umbilical cord, and placenta. This work was supported by the Li Ka Shing Institute of Health Sciences Grant and RGC project: CUHK 7422_03M.

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorLi, Gen_HK
dc.contributor.authorZhang, XAen_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorMeng, CLen_HK
dc.contributor.authorChan, CYen_HK
dc.contributor.authorYew, DTWen_HK
dc.contributor.authorTsang, KSen_HK
dc.contributor.authorLi, Ken_HK
dc.contributor.authorTsai, SNen_HK
dc.contributor.authorNgai, SMen_HK
dc.contributor.authorHan, ZCen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorHe, MLen_HK
dc.contributor.authorKung, HFen_HK
dc.date.accessioned2010-05-31T03:30:27Z-
dc.date.available2010-05-31T03:30:27Z-
dc.date.issued2009en_HK
dc.identifier.citationProteomics, 2009, v. 9 n. 1, p. 20-30en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58446-
dc.description.abstractUmbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) as sources of mesenchymal stem cells (MSC) for cell therapy, with both UC- and P-MSC possess immunophenotypic and functional characteristics similar to BM-MSC. However, their migration capacity, which is indispensable during tissue regeneration process, is unclear. Under defined conditions, the migration capacity of BM- and P-MSC was found 5.9- and 3.2-folds higher than that of UC-MSC, respectively. By the use of 2-DE and combined MS and MS/MS analysis, six differentially expressed proteins were identified among these MSC samples, with five of them known to be involved in cell migration as migration enhancing or inhibiting proteins. Consistent with their migration capacity, the levels ofmigration enhancing proteins including cathepsin B, cathepsin D and prohibitin,were significantly lower in UC-MSC when compared with those in BM- and P-MSC. For the migration inhibiting proteins such as plasminogen activator inhibitor-1 (PAI-1) and manganese superoxide dismutase, higher expression was found in the UC-MSC. We also showed that the overexpression of the PAI-1 impaired the migration capacity of BM- and P- MSC while silencing of PAI-1 enhanced the migration capacity of UC-MSC. Our study indicates that PAI-1 and other migration-related proteins are pivotal in governing the migration capacity of MSC. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subjectBone marrowen_HK
dc.subjectMesenchymal stem cellsen_HK
dc.subjectMigrationen_HK
dc.subjectPlacentaen_HK
dc.subjectUmbilical corden_HK
dc.titleComparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord, and placenta: Implication in the migrationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9853&volume=9&issue=1&spage=20&epage=30&date=2008&atitle=Comparative+Proteomic+Analysis+of+Mesenchymal+Stem+Cells+Derived+from+Human+Bone+Marrow,+Umbilical+Cord,+and+Placenta:+Implication+in+the+Migrationen_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200701195en_HK
dc.identifier.pmid19116983en_HK
dc.identifier.scopuseid_2-s2.0-59449100237en_HK
dc.identifier.hkuros154166en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-59449100237&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue1en_HK
dc.identifier.spage20en_HK
dc.identifier.epage30en_HK
dc.identifier.isiWOS:000262568500003-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLi, G=7407055832en_HK
dc.identifier.scopusauthoridZhang, XA=22996102400en_HK
dc.identifier.scopusauthoridWang, H=7501747965en_HK
dc.identifier.scopusauthoridWang, X=7501851697en_HK
dc.identifier.scopusauthoridMeng, CL=36927269600en_HK
dc.identifier.scopusauthoridChan, CY=22033276600en_HK
dc.identifier.scopusauthoridYew, DTW=7007034694en_HK
dc.identifier.scopusauthoridTsang, KS=7201555004en_HK
dc.identifier.scopusauthoridLi, K=7404990071en_HK
dc.identifier.scopusauthoridTsai, SN=8707094300en_HK
dc.identifier.scopusauthoridNgai, SM=7006074219en_HK
dc.identifier.scopusauthoridHan, ZC=7402859036en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridHe, ML=35080389700en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.citeulike5791453-
dc.identifier.issnl1615-9853-

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