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Article: Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9

TitleExpression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
Authors
Wang, WWei, LYang, AHe, TYuen, KYChen, CRao, Z
KeywordsCoronaviruses
Human coronavirus HKU1
Nonstructural proteins
Issue Date2009
PublisherInternational Union of Crystallography. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291744-3091
Citation
Acta Crystallographica Section F: Structural Biology and Crystallization Communications Online, 2009, v. 65 n. 5, p. 526-528 How to Cite?
DOI: http://dx.doi.org/10.1107/S1744309109014055
AbstractHuman coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit.
Persistent Identifierhttp://hdl.handle.net/10722/59400
ISSN
1744-3091
2014 Impact Factor: 0.524
PubMed Central ID
PMC2675602
ISI Accession Number ID
WOS:000265639600025

 

DC FieldValueLanguage
dc.contributor.authorWang, Wen_HK
dc.contributor.authorWei, Len_HK
dc.contributor.authorYang, Aen_HK
dc.contributor.authorHe, Ten_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorChen, Cen_HK
dc.contributor.authorRao, Zen_HK
dc.date.accessioned2010-05-31T03:49:20Z-
dc.date.available2010-05-31T03:49:20Z-
dc.date.issued2009en_HK
dc.identifier.citationActa Crystallographica Section F: Structural Biology and Crystallization Communications Online, 2009, v. 65 n. 5, p. 526-528en_HK
dc.identifier.issn1744-3091-
dc.identifier.urihttp://hdl.handle.net/10722/59400-
dc.description.abstractHuman coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit.-
dc.languageengen_HK
dc.publisherInternational Union of Crystallography. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291744-3091-
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications Onlineen_HK
dc.subjectCoronaviruses-
dc.subjectHuman coronavirus HKU1-
dc.subjectNonstructural proteins-
dc.subject.meshCrystallography, X-Ray-
dc.subject.meshGene Expression-
dc.subject.meshRNA-Binding Proteins - chemistry - genetics - metabolism-
dc.subject.meshSARS Virus - chemistry - genetics - metabolism-
dc.subject.meshViral Proteins - chemistry - genetics - metabolism-
dc.titleExpression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9en_HK
dc.typeArticleen_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.emailChen, C: chench@mail.xtal.tsinghua.edu.cn-
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1107/S1744309109014055-
dc.identifier.pmid19407394-
dc.identifier.pmcidPMC2675602-
dc.identifier.scopuseid_2-s2.0-67249102368-
dc.identifier.hkuros167084en_HK
dc.identifier.volume65-
dc.identifier.issue5-
dc.identifier.spage526-
dc.identifier.epage528-
dc.identifier.isiWOS:000265639600025-
dc.publisher.placeUnited States-
dc.identifier.citeulike4441790-
dc.identifier.issnl1744-3091-

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