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Article: Expression of trkA, trkB, and trkC in injured and regenerating retinal ganglion cells of adult rats
Title | Expression of trkA, trkB, and trkC in injured and regenerating retinal ganglion cells of adult rats |
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Authors | |
Issue Date | 2002 |
Publisher | Association for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org |
Citation | Investigative Ophthalmology And Visual Science, 2002, v. 43 n. 6, p. 1954-1964 How to Cite? |
Abstract | PURPOSE. To investigate changes in percentage of tyrosine kinase (trk)A-, trkB-, and trkC-immunopositive ( +) retinal ganglion cells (RGCs) at various times after optic nerve (ON) axotomy; the proportion of RGCs regenerating axons into peripheral nerve (PN) grafts that are trkA +, trkB +, and trkC +; whether intravitreal PN-ON implants affect trk immunoreactivity; and the levels of trk mRNAs in ON-injured retinas. METHODS. The ON was transected intraorbitally. Proportions of trkA +, trkB +, and trkC + RGCs and levels of trk mRNAs were studied by using immunocytochemistry and Northern blot methods, respectively, in injured and RGC-regenerating retinas. RESULTS. In normal retinas, only small numbers of trkB + and trkC +, but not trkA +, RGCs were seen. The optic fiber layer was intensively immunolabeled with trkB. After ON injury, the proportions of trkA +, trkB +, and trkC + RGCs rapidly increased and reached their peaks by 3 to 5 days. During the next 3 weeks, the proportion of trkA + or trkB + RGCs gradually decreased, but the proportion of trkC + RGCs remained high. Intravitreal implants of PN+ON segments transiently but significantly suppressed injury-induced increases in all these trk + RGC proportions for approximately 5 days. In contrast, 3 days after ON injury, quantitative retinal expression of trkA mRNA, and to a lesser extent trkC mRNA, was downregulated, whereas trkB mRNA expression remained unaffected. Higher proportions of trkA + and trkB + RGCs and higher levels of all trk mRNAs were seen in regenerating RGCs and retinas, respectively. CONCLUSIONS. This study provides a kinetic analysis of expression of trk in RGCs and retinas after ON injury and during regeneration. |
Persistent Identifier | http://hdl.handle.net/10722/67810 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.422 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cui, Q | en_HK |
dc.contributor.author | Tang, LS | en_HK |
dc.contributor.author | Hu, B | en_HK |
dc.contributor.author | So, KF | en_HK |
dc.contributor.author | Yip, HK | en_HK |
dc.date.accessioned | 2010-09-06T05:58:27Z | - |
dc.date.available | 2010-09-06T05:58:27Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | Investigative Ophthalmology And Visual Science, 2002, v. 43 n. 6, p. 1954-1964 | en_HK |
dc.identifier.issn | 0146-0404 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67810 | - |
dc.description.abstract | PURPOSE. To investigate changes in percentage of tyrosine kinase (trk)A-, trkB-, and trkC-immunopositive ( +) retinal ganglion cells (RGCs) at various times after optic nerve (ON) axotomy; the proportion of RGCs regenerating axons into peripheral nerve (PN) grafts that are trkA +, trkB +, and trkC +; whether intravitreal PN-ON implants affect trk immunoreactivity; and the levels of trk mRNAs in ON-injured retinas. METHODS. The ON was transected intraorbitally. Proportions of trkA +, trkB +, and trkC + RGCs and levels of trk mRNAs were studied by using immunocytochemistry and Northern blot methods, respectively, in injured and RGC-regenerating retinas. RESULTS. In normal retinas, only small numbers of trkB + and trkC +, but not trkA +, RGCs were seen. The optic fiber layer was intensively immunolabeled with trkB. After ON injury, the proportions of trkA +, trkB +, and trkC + RGCs rapidly increased and reached their peaks by 3 to 5 days. During the next 3 weeks, the proportion of trkA + or trkB + RGCs gradually decreased, but the proportion of trkC + RGCs remained high. Intravitreal implants of PN+ON segments transiently but significantly suppressed injury-induced increases in all these trk + RGC proportions for approximately 5 days. In contrast, 3 days after ON injury, quantitative retinal expression of trkA mRNA, and to a lesser extent trkC mRNA, was downregulated, whereas trkB mRNA expression remained unaffected. Higher proportions of trkA + and trkB + RGCs and higher levels of all trk mRNAs were seen in regenerating RGCs and retinas, respectively. CONCLUSIONS. This study provides a kinetic analysis of expression of trk in RGCs and retinas after ON injury and during regeneration. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Association for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org | en_HK |
dc.relation.ispartof | Investigative Ophthalmology and Visual Science | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Axotomy | en_HK |
dc.subject.mesh | Blotting, Northern | en_HK |
dc.subject.mesh | Cell Survival | en_HK |
dc.subject.mesh | DNA Probes | en_HK |
dc.subject.mesh | Down-Regulation | en_HK |
dc.subject.mesh | Fluorescent Antibody Technique, Indirect | en_HK |
dc.subject.mesh | Nerve Regeneration - physiology | en_HK |
dc.subject.mesh | Optic Nerve - physiology | en_HK |
dc.subject.mesh | Optic Nerve Injuries - enzymology | en_HK |
dc.subject.mesh | RNA, Messenger - metabolism | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.subject.mesh | Receptor, trkA - genetics - metabolism | en_HK |
dc.subject.mesh | Receptor, trkB - genetics - metabolism | en_HK |
dc.subject.mesh | Receptor, trkC - genetics - metabolism | en_HK |
dc.subject.mesh | Retina - metabolism | en_HK |
dc.subject.mesh | Retinal Ganglion Cells - enzymology | en_HK |
dc.subject.mesh | Time Factors | en_HK |
dc.title | Expression of trkA, trkB, and trkC in injured and regenerating retinal ganglion cells of adult rats | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | So, KF:hrmaskf@hkucc.hku.hk | en_HK |
dc.identifier.email | Yip, HK:hkfyip@hku.hk | en_HK |
dc.identifier.authority | So, KF=rp00329 | en_HK |
dc.identifier.authority | Yip, HK=rp00285 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.pmid | 12037005 | - |
dc.identifier.scopus | eid_2-s2.0-0036271504 | en_HK |
dc.identifier.hkuros | 67575 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036271504&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 43 | en_HK |
dc.identifier.issue | 6 | en_HK |
dc.identifier.spage | 1954 | en_HK |
dc.identifier.epage | 1964 | en_HK |
dc.identifier.isi | WOS:000175927800040 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Cui, Q=7103080164 | en_HK |
dc.identifier.scopusauthorid | Tang, LS=7402081562 | en_HK |
dc.identifier.scopusauthorid | Hu, B=35733928400 | en_HK |
dc.identifier.scopusauthorid | So, KF=34668391300 | en_HK |
dc.identifier.scopusauthorid | Yip, HK=7101980864 | en_HK |
dc.identifier.issnl | 0146-0404 | - |