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Article: Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury
Title | Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury |
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Authors | |
Keywords | Inflammation Lipid peroxidation Liver injury Nitric oxide Oxidative stress |
Issue Date | 2006 |
Publisher | Histology and Histopathology. The Journal's web site is located at http://www.hh.um.es |
Citation | Histology And Histopathology, 2006, v. 21 n. 10-12, p. 1157-1165 How to Cite? |
Abstract | The exact functional role of nitric oxide (NO) in liver injury is currently a source of controversy. NO is enzymatically synthesized by nitric oxide synthase (NOS). In this study, we assessed the role of inducible NOS (iNOS) in carbon tetrachloride (CCl 4)-induced acute liver injury using inhibitors of iNOS, and an NO donor. Adult ICR mice were injected with CCl 4 with or without the iNOS inhibitors (5-methylisothiourea hemisulfate [SMT] and 1-N 6-(1-iminoethyl)-lysine [L-NIL]) and an NO donor (Sodium Nitroprusside [SNP]). Blood and liver tissues were collected for analysis. Immunohistochemistry (IHC), serum alanine aminotransferase (ALT), serum total 8-isoprostane analysis, RT-PCR, Western Blotting (WB) and EMSA were done. Our results showed increased levels of ALT, necrosis, total 8-isoprostane and nitrotyrosine after CCl 4 administration. iNOS inhibitors and SNP abrogated these effects but the effect was more pronounced with SMT and L-NIL. RT-PCR, WB and IHC in CCl 4-treated mice demonstrated upregulation of TNF-α, iNOS, and COX-2. The administration of iNOS inhibitors with CCl 4 diminished the expression of these proinflammatory mediators. NF-κB was also upregulated in CCl 4-treated mice and was reversed in mice pretreated wil iNOS inhibitors. SNP pretreated mice also showed a lower expression of COX-2 when compared with CCl 4 treated mice but TNF-α, iNOS and NF-κB activity were unaffected. We propose that a high level of nitric oxide is associated with CCl 4-induced acute liver injury and the liver injury can be ameliorated by decreasing the NO level with iNOS inhibitors and an NO donor with the former more effective in reducing CCl 4-induced liver injury. |
Persistent Identifier | http://hdl.handle.net/10722/67881 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.571 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tipoe, GL | en_HK |
dc.contributor.author | Leung, TM | en_HK |
dc.contributor.author | Liong, E | en_HK |
dc.contributor.author | So, H | en_HK |
dc.contributor.author | Leung, KM | en_HK |
dc.contributor.author | Lau, TYH | en_HK |
dc.contributor.author | Tom, WM | en_HK |
dc.contributor.author | Fung, ML | en_HK |
dc.contributor.author | Fan, ST | en_HK |
dc.contributor.author | Nanji, AA | en_HK |
dc.date.accessioned | 2010-09-06T05:59:07Z | - |
dc.date.available | 2010-09-06T05:59:07Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Histology And Histopathology, 2006, v. 21 n. 10-12, p. 1157-1165 | en_HK |
dc.identifier.issn | 0213-3911 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67881 | - |
dc.description.abstract | The exact functional role of nitric oxide (NO) in liver injury is currently a source of controversy. NO is enzymatically synthesized by nitric oxide synthase (NOS). In this study, we assessed the role of inducible NOS (iNOS) in carbon tetrachloride (CCl 4)-induced acute liver injury using inhibitors of iNOS, and an NO donor. Adult ICR mice were injected with CCl 4 with or without the iNOS inhibitors (5-methylisothiourea hemisulfate [SMT] and 1-N 6-(1-iminoethyl)-lysine [L-NIL]) and an NO donor (Sodium Nitroprusside [SNP]). Blood and liver tissues were collected for analysis. Immunohistochemistry (IHC), serum alanine aminotransferase (ALT), serum total 8-isoprostane analysis, RT-PCR, Western Blotting (WB) and EMSA were done. Our results showed increased levels of ALT, necrosis, total 8-isoprostane and nitrotyrosine after CCl 4 administration. iNOS inhibitors and SNP abrogated these effects but the effect was more pronounced with SMT and L-NIL. RT-PCR, WB and IHC in CCl 4-treated mice demonstrated upregulation of TNF-α, iNOS, and COX-2. The administration of iNOS inhibitors with CCl 4 diminished the expression of these proinflammatory mediators. NF-κB was also upregulated in CCl 4-treated mice and was reversed in mice pretreated wil iNOS inhibitors. SNP pretreated mice also showed a lower expression of COX-2 when compared with CCl 4 treated mice but TNF-α, iNOS and NF-κB activity were unaffected. We propose that a high level of nitric oxide is associated with CCl 4-induced acute liver injury and the liver injury can be ameliorated by decreasing the NO level with iNOS inhibitors and an NO donor with the former more effective in reducing CCl 4-induced liver injury. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Histology and Histopathology. The Journal's web site is located at http://www.hh.um.es | en_HK |
dc.relation.ispartof | Histology and Histopathology | en_HK |
dc.subject | Inflammation | en_HK |
dc.subject | Lipid peroxidation | en_HK |
dc.subject | Liver injury | en_HK |
dc.subject | Nitric oxide | en_HK |
dc.subject | Oxidative stress | en_HK |
dc.title | Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0213-3911&volume=21&issue=11&spage=1157&epage=1165&date=2006&atitle=Inhibitors+of+inducible+nitric+oxide+(NO)+synthase+are+more+effective+than+an+NO+donor+in+reducing+carbon-tetrachloride+induced+acute+liver+injury | en_HK |
dc.identifier.email | Tipoe, GL: tgeorge@hkucc.hku.hk | en_HK |
dc.identifier.email | Tom, WM: wmtoma@hkucc.hku.hk | en_HK |
dc.identifier.email | Fung, ML: fungml@hkucc.hku.hk | en_HK |
dc.identifier.email | Fan, ST: stfan@hku.hk | en_HK |
dc.identifier.authority | Tipoe, GL=rp00371 | en_HK |
dc.identifier.authority | Tom, WM=rp00237 | en_HK |
dc.identifier.authority | Fung, ML=rp00433 | en_HK |
dc.identifier.authority | Fan, ST=rp00355 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.pmid | 16874658 | - |
dc.identifier.scopus | eid_2-s2.0-33750299548 | en_HK |
dc.identifier.hkuros | 117709 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33750299548&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 21 | en_HK |
dc.identifier.issue | 10-12 | en_HK |
dc.identifier.spage | 1157 | en_HK |
dc.identifier.epage | 1165 | en_HK |
dc.identifier.isi | WOS:000239239800003 | - |
dc.publisher.place | Spain | en_HK |
dc.identifier.scopusauthorid | Tipoe, GL=7003550610 | en_HK |
dc.identifier.scopusauthorid | Leung, TM=7202110149 | en_HK |
dc.identifier.scopusauthorid | Liong, E=6602732210 | en_HK |
dc.identifier.scopusauthorid | So, H=14068993500 | en_HK |
dc.identifier.scopusauthorid | Leung, KM=7401860685 | en_HK |
dc.identifier.scopusauthorid | Lau, TYH=26323763000 | en_HK |
dc.identifier.scopusauthorid | Tom, WM=7003709519 | en_HK |
dc.identifier.scopusauthorid | Fung, ML=7101955092 | en_HK |
dc.identifier.scopusauthorid | Fan, ST=7402678224 | en_HK |
dc.identifier.scopusauthorid | Nanji, AA=35885060300 | en_HK |
dc.identifier.issnl | 0213-3911 | - |