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Article: Separation and purification of phycocyanins from Spirulina platensis in mixotrophic culture
| Title | Separation and purification of phycocyanins from Spirulina platensis in mixotrophic culture 混合异养螺旋藻中藻蓝蛋白的分离和纯化 |
|---|---|
| Authors | |
| Keywords | Separation Spirulina platensis Purification Phycocyanin |
| Issue Date | 1999 |
| Publisher | South China University of Technology. The Journal's web site is located at http://www.cnki.com.cn/Journal/C-C8-HNLG.htm |
| Citation | 華南理工大學學報(自然科學版), 1999, v. 27 n. 4, p. 32-36 How to Cite? Journal of South China University of Technology (Natural Science Edition), 1999, v. 27 n. 4, p. 32-36 How to Cite? |
| Abstract | For phycocyanin separation and purification of Spirulina platensis, a method using fraction precipitation of ammonium sulphate, ion exchange and gel filtration chromatography was investigated. In fraction precipitation of ammonium sulphate, it was found that by adsorption spectra scan of precipitates, 300 g/ L ammonium sulphate solution could remove other proteins well while 500 g/ L and 650 g/ L ammonium sulphate solutions could separate c_phycocy anin and allophycocy anin well. After ion exchange chromatography and gel filtration chromatography, finally , the absorbance ratios, reflecting the purities of c_phy cocyanin and allophy cocyanin, reached 5.06 and 5 .34 respectively . |
| Persistent Identifier | http://hdl.handle.net/10722/68576 |
| ISSN | 2020 SCImago Journal Rankings: 0.154 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Zhang, YM | en_HK |
| dc.contributor.author | Guo, SY | en_HK |
| dc.contributor.author | Chen, SF | en_HK |
| dc.date.accessioned | 2010-09-06T06:05:49Z | - |
| dc.date.available | 2010-09-06T06:05:49Z | - |
| dc.date.issued | 1999 | en_HK |
| dc.identifier.citation | 華南理工大學學報(自然科學版), 1999, v. 27 n. 4, p. 32-36 | en_HK |
| dc.identifier.citation | Journal of South China University of Technology (Natural Science Edition), 1999, v. 27 n. 4, p. 32-36 | - |
| dc.identifier.issn | 1000-565X | en_HK |
| dc.identifier.uri | http://hdl.handle.net/10722/68576 | - |
| dc.description.abstract | For phycocyanin separation and purification of Spirulina platensis, a method using fraction precipitation of ammonium sulphate, ion exchange and gel filtration chromatography was investigated. In fraction precipitation of ammonium sulphate, it was found that by adsorption spectra scan of precipitates, 300 g/ L ammonium sulphate solution could remove other proteins well while 500 g/ L and 650 g/ L ammonium sulphate solutions could separate c_phycocy anin and allophycocy anin well. After ion exchange chromatography and gel filtration chromatography, finally , the absorbance ratios, reflecting the purities of c_phy cocyanin and allophy cocyanin, reached 5.06 and 5 .34 respectively . | - |
| dc.language | eng | en_HK |
| dc.publisher | South China University of Technology. The Journal's web site is located at http://www.cnki.com.cn/Journal/C-C8-HNLG.htm | en_HK |
| dc.relation.ispartof | 華南理工大學學報(自然科學版) | en_HK |
| dc.relation.ispartof | Journal of South China University of Technology (Natural Science Edition) | - |
| dc.subject | Separation | - |
| dc.subject | Spirulina platensis | - |
| dc.subject | Purification | - |
| dc.subject | Phycocyanin | - |
| dc.title | Separation and purification of phycocyanins from Spirulina platensis in mixotrophic culture | en_HK |
| dc.title | 混合异养螺旋藻中藻蓝蛋白的分离和纯化 | - |
| dc.type | Article | en_HK |
| dc.identifier.email | Chen, SF: sfchen@hkusua.hku.hk | en_HK |
| dc.identifier.authority | Chen, SF=rp00672 | en_HK |
| dc.identifier.hkuros | 45474 | en_HK |
| dc.identifier.volume | 27 | - |
| dc.identifier.issue | 4 | - |
| dc.identifier.spage | 32 | - |
| dc.identifier.epage | 36 | - |
| dc.publisher.place | China | - |
| dc.identifier.issnl | 1000-565X | - |