File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1158/0008-5472.CAN-06-1227
- Scopus: eid_2-s2.0-33846260583
- PMID: 17178893
- WOS: WOS:000242915600053
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Therapeutic expression of an anti-death receptor 5 single-chain fixed-variable region prevents tumor growth in mice
Title | Therapeutic expression of an anti-death receptor 5 single-chain fixed-variable region prevents tumor growth in mice |
---|---|
Authors | |
Issue Date | 2006 |
Publisher | American Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/ |
Citation | Cancer Research, 2006, v. 66 n. 24, p. 11946-11953 How to Cite? |
Abstract | The clinical use of the single-chain fixed-variable (scFv) fragments of recombinant monoclonal antibodies as credible alternatives for classic therapeutic antibodies has two limitations: rapid blood clearance and inefficient local expression of functional molecules. In attempt to address these issues, we have developed a novel gene therapy protocol in which the anti-death receptor 5 (DR5) scFv fragments were either in vitro expressed in several tumor cell lines, or in vivo expressed in mice, using recombinant adeno-associated virus (rAAV)-mediated gene transfer. Viral transduction using the rAAV-S3C construct, which encodes a scFv molecule (S3C scFv) specific to DR5, led to stable expression in tumor cell lines and showed apoptosis-inducing activity in vitro, which could be inhibited by recombinant DR5 but not by DR4. A single i.m. injection of rAAV-S3C virus in nude mice resulted in stable expression of DR5-binding S3C scFv proteins in mouse sera for at least 240 days. Moreover, the expression of S3C scFv was associated with significant suppression of tumor growth and the increase of tumor cell apoptosis in previously established s.c. human lung LTEP-sml and liver Hep3B tumor xenografts. ©2006 American Association for Cancer Research. |
Persistent Identifier | http://hdl.handle.net/10722/80110 |
ISSN | 2023 Impact Factor: 12.5 2023 SCImago Journal Rankings: 3.468 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shi, J | en_HK |
dc.contributor.author | Liu, Y | en_HK |
dc.contributor.author | Zheng, Y | en_HK |
dc.contributor.author | Guo, Y | en_HK |
dc.contributor.author | Zhang, J | en_HK |
dc.contributor.author | Cheung, PT | en_HK |
dc.contributor.author | Xu, R | en_HK |
dc.contributor.author | Zheng, D | en_HK |
dc.date.accessioned | 2010-09-06T08:02:32Z | - |
dc.date.available | 2010-09-06T08:02:32Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Cancer Research, 2006, v. 66 n. 24, p. 11946-11953 | en_HK |
dc.identifier.issn | 0008-5472 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/80110 | - |
dc.description.abstract | The clinical use of the single-chain fixed-variable (scFv) fragments of recombinant monoclonal antibodies as credible alternatives for classic therapeutic antibodies has two limitations: rapid blood clearance and inefficient local expression of functional molecules. In attempt to address these issues, we have developed a novel gene therapy protocol in which the anti-death receptor 5 (DR5) scFv fragments were either in vitro expressed in several tumor cell lines, or in vivo expressed in mice, using recombinant adeno-associated virus (rAAV)-mediated gene transfer. Viral transduction using the rAAV-S3C construct, which encodes a scFv molecule (S3C scFv) specific to DR5, led to stable expression in tumor cell lines and showed apoptosis-inducing activity in vitro, which could be inhibited by recombinant DR5 but not by DR4. A single i.m. injection of rAAV-S3C virus in nude mice resulted in stable expression of DR5-binding S3C scFv proteins in mouse sera for at least 240 days. Moreover, the expression of S3C scFv was associated with significant suppression of tumor growth and the increase of tumor cell apoptosis in previously established s.c. human lung LTEP-sml and liver Hep3B tumor xenografts. ©2006 American Association for Cancer Research. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/ | en_HK |
dc.relation.ispartof | Cancer Research | en_HK |
dc.title | Therapeutic expression of an anti-death receptor 5 single-chain fixed-variable region prevents tumor growth in mice | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=66&issue=24&spage=11946&epage=11953&date=2006&atitle=Therapeutic+Expression+of+an+Anti-Death+Receptor+5+Single-Chain+Fixed-Variable+Region+Prevents+Tumor+Growth+in+Mice | en_HK |
dc.identifier.email | Cheung, PT:ptcheung@hkucc.hku.hk | en_HK |
dc.identifier.authority | Cheung, PT=rp00351 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-06-1227 | en_HK |
dc.identifier.pmid | 17178893 | en_HK |
dc.identifier.scopus | eid_2-s2.0-33846260583 | en_HK |
dc.identifier.hkuros | 127715 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33846260583&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 66 | en_HK |
dc.identifier.issue | 24 | en_HK |
dc.identifier.spage | 11946 | en_HK |
dc.identifier.epage | 11953 | en_HK |
dc.identifier.isi | WOS:000242915600053 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Shi, J=7404495444 | en_HK |
dc.identifier.scopusauthorid | Liu, Y=8232975800 | en_HK |
dc.identifier.scopusauthorid | Zheng, Y=7404837714 | en_HK |
dc.identifier.scopusauthorid | Guo, Y=10540367500 | en_HK |
dc.identifier.scopusauthorid | Zhang, J=15766639800 | en_HK |
dc.identifier.scopusauthorid | Cheung, PT=7202595465 | en_HK |
dc.identifier.scopusauthorid | Xu, R=16426308500 | en_HK |
dc.identifier.scopusauthorid | Zheng, D=7202567084 | en_HK |
dc.identifier.issnl | 0008-5472 | - |