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- Scopus: eid_2-s2.0-0242390523
- PMID: 14627487
- WOS: WOS:000186505500002
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Article: Low molecular weight G-proteins of rho-family mediate relaxations to bradykinin in porcine coronary arteries
Title | Low molecular weight G-proteins of rho-family mediate relaxations to bradykinin in porcine coronary arteries |
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Authors | |
Keywords | Bradykinin Endothelium GTP-binding proteins Nitric oxide |
Issue Date | 2003 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html |
Citation | Acta Pharmacologica Sinica, 2003, v. 24 n. 11, p. 1070-1076 How to Cite? |
Abstract | AIM: To determine whether or not low molecular G-proteins are involved in the endothelium-dependent relaxations to bradykinin. METHODS: The effects of botulinum ADP-ribosyltranferase C3 were studied in porcine coronary arteries and endothelial cells. RESULTS: Incubation of membrane fractions isolated from endothelial cells with the enzyme and 32P-NAD resulted in the ribosylation of the proteins with molecular weight of 24-25 kDa. Radio labelling of these proteins was suppressed in the presence of guanosine 5′-O-(3-thiotriphosphate) (GTP-γS), a hydrolysis-resistant analog of GTP. In the isolated arteries, ADP-ribosyltransferase C3 attenuated the relaxations to bradykinin during contractions with prostaglandin F 2α in the presence of tween 80 (non ionic detergent), but not in the absence of tween 80. CONCLUSION: Low molecular weight G-proteins of the Rho family contribute to the mechanism of relaxation induced by bradykinin. |
Persistent Identifier | http://hdl.handle.net/10722/80207 |
ISSN | 2023 Impact Factor: 6.9 2023 SCImago Journal Rankings: 1.882 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Shibano, T | en_HK |
dc.contributor.author | Vanhoutte, PM | en_HK |
dc.date.accessioned | 2010-09-06T08:03:43Z | - |
dc.date.available | 2010-09-06T08:03:43Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Acta Pharmacologica Sinica, 2003, v. 24 n. 11, p. 1070-1076 | en_HK |
dc.identifier.issn | 1671-4083 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/80207 | - |
dc.description.abstract | AIM: To determine whether or not low molecular G-proteins are involved in the endothelium-dependent relaxations to bradykinin. METHODS: The effects of botulinum ADP-ribosyltranferase C3 were studied in porcine coronary arteries and endothelial cells. RESULTS: Incubation of membrane fractions isolated from endothelial cells with the enzyme and 32P-NAD resulted in the ribosylation of the proteins with molecular weight of 24-25 kDa. Radio labelling of these proteins was suppressed in the presence of guanosine 5′-O-(3-thiotriphosphate) (GTP-γS), a hydrolysis-resistant analog of GTP. In the isolated arteries, ADP-ribosyltransferase C3 attenuated the relaxations to bradykinin during contractions with prostaglandin F 2α in the presence of tween 80 (non ionic detergent), but not in the absence of tween 80. CONCLUSION: Low molecular weight G-proteins of the Rho family contribute to the mechanism of relaxation induced by bradykinin. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html | en_HK |
dc.relation.ispartof | Acta Pharmacologica Sinica | en_HK |
dc.subject | Bradykinin | en_HK |
dc.subject | Endothelium | en_HK |
dc.subject | GTP-binding proteins | en_HK |
dc.subject | Nitric oxide | en_HK |
dc.title | Low molecular weight G-proteins of rho-family mediate relaxations to bradykinin in porcine coronary arteries | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1671-4083&volume=24&issue=11&spage=1070&epage=1076&date=2003&atitle=Low+molecular+weight+G-proteins+of+rho-family+mediate+relaxations+to+bradykinin+in+porcine+coronary+arteries | en_HK |
dc.identifier.email | Vanhoutte, PM: vanhoutt@hku.hk | en_HK |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.pmid | 14627487 | - |
dc.identifier.scopus | eid_2-s2.0-0242390523 | en_HK |
dc.identifier.hkuros | 95755 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0242390523&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 24 | en_HK |
dc.identifier.issue | 11 | en_HK |
dc.identifier.spage | 1070 | en_HK |
dc.identifier.epage | 1076 | en_HK |
dc.identifier.isi | WOS:000186505500002 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Shibano, T=7006946465 | en_HK |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_HK |
dc.identifier.issnl | 1671-4083 | - |