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Article: Chicken Infectious Diseases Virus Recombinant DNA Constructs and its Efficacy in Special Pathogen Free Chickens

TitleChicken Infectious Diseases Virus Recombinant DNA Constructs and its Efficacy in Special Pathogen Free Chickens
Authors
Wu, HChen, JLeung, FCC
Issue Date2003
PublisherAsian Network for Scientific Information (A N S I N E T). The Journal's web site is located at http://www.medwellonline.net/java/fp.html
Citation
Journal of Animal and Veterinary Advances, 2003, v. 2 n. 8, p. 448-451 How to Cite?
AbstractIn order to determine the effective region of large segment genome of infectious bursal disease virus for the preparation of DNA vaccine, four-recombinant DNA constructs with various combination of VP2 and VP5 fragments have been inserted into plasmid pcDNA3.1 were constructed. They were designated as VP5-5.1+VP2-3.4 (A), VP5-5.2+VP2-3.4 (B), VP2-2.5+VP2-3.4(C) and VP2-5.3+VP2-3.3 (D) respectively. Immunization assays was conducted using 1-week-old SPF chickens and DNA constructs were delivered in two ways, intramuscularly injections or via the gene gun bombardments. Chickens were primed with 100ug DNA, then boosted were given at 7days interval for two times. The chickens were finally challenged with a IBDV HK46 strain. After analyzing the neutralizing antibody and bursa-body weight ratios, the recombinant construct VP5-5.2+VP2-3.4 was found to be the most effective when given through intramuscularly injections or via the gene gun bombardments.
Persistent Identifierhttp://hdl.handle.net/10722/84856
ISSN
1680-5593
2012 Impact Factor: 0.365

 

DC FieldValueLanguage
dc.contributor.authorWu, Hen_HK
dc.contributor.authorChen, Jen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T08:57:55Z-
dc.date.available2010-09-06T08:57:55Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal of Animal and Veterinary Advances, 2003, v. 2 n. 8, p. 448-451en_HK
dc.identifier.issn1680-5593en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84856-
dc.description.abstractIn order to determine the effective region of large segment genome of infectious bursal disease virus for the preparation of DNA vaccine, four-recombinant DNA constructs with various combination of VP2 and VP5 fragments have been inserted into plasmid pcDNA3.1 were constructed. They were designated as VP5-5.1+VP2-3.4 (A), VP5-5.2+VP2-3.4 (B), VP2-2.5+VP2-3.4(C) and VP2-5.3+VP2-3.3 (D) respectively. Immunization assays was conducted using 1-week-old SPF chickens and DNA constructs were delivered in two ways, intramuscularly injections or via the gene gun bombardments. Chickens were primed with 100ug DNA, then boosted were given at 7days interval for two times. The chickens were finally challenged with a IBDV HK46 strain. After analyzing the neutralizing antibody and bursa-body weight ratios, the recombinant construct VP5-5.2+VP2-3.4 was found to be the most effective when given through intramuscularly injections or via the gene gun bombardments.-
dc.languageengen_HK
dc.publisherAsian Network for Scientific Information (A N S I N E T). The Journal's web site is located at http://www.medwellonline.net/java/fp.htmlen_HK
dc.relation.ispartofJournal of Animal and Veterinary Advancesen_HK
dc.titleChicken Infectious Diseases Virus Recombinant DNA Constructs and its Efficacy in Special Pathogen Free Chickensen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1680-5593&volume=2&spage=448&epage=451&date=2003&atitle=Chicken+Infectious+Diseases+Virus+Recombinant+DNA+Constructs+and+its+Efficacy+in+Special+Pathogen+Free+Chickensen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.hkuros85848en_HK
dc.identifier.issnl1680-5593-

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