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- Publisher Website: 10.1074/jbc.C109.023937
- Scopus: eid_2-s2.0-69249088889
- PMID: 19553677
- WOS: WOS:000268564400005
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Article: MRG15 is a novel PALB2-interacting factor involved in homologous recombination
Title | MRG15 is a novel PALB2-interacting factor involved in homologous recombination | ||||
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Authors | |||||
Keywords | Chemicals And Cas Registry Numbers | ||||
Issue Date | 2009 | ||||
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | ||||
Citation | Journal Of Biological Chemistry, 2009, v. 284 n. 32, p. 21127-21131 How to Cite? | ||||
Abstract | PALB2 is an integral component of the BRCA complex important for recombinational DNA repair. However, exactly how this activity is regulated in vivo remains unexplored. Here we provide evidefice to show that MRG15 is a novel PALB2-associated protein that ensures regulated recombination events. We found that the direct interaction between MRG15 and PALB2 is mediated by an evolutionarily conserved region on PALB2. Intriguingly, although damage-induced RAD51 foci formation and mitomycin C sensitivity appeared normal in MRG15-binding defective PALB2 mutants, these cells exhibited a significant increase in gene conversion rates. Consistently, we found that abrogation of the PALB2-MRG15 interaction resulted in elevated sister chromatid exchange frequencies. Our results suggest that loss of the PALB2-ARG15 interaction relieved the cells with the suppression of sister chromatid exchange and therefore led to a hyper-recombination phenotype in the gene conversion assay. Together, our study indicated that although PALB2 is required for proficient homologous recombination, it could also govern the choice of templates used in homologous recombination repair. © 2009 by The American Society for Biochemistry and Molecular Biology, Inc. | ||||
Persistent Identifier | http://hdl.handle.net/10722/90930 | ||||
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 | ||||
PubMed Central ID | |||||
ISI Accession Number ID |
Funding Information: This work was supported, in whole or in part, by National Institutes of Health Grants CA089239, CA092312 and CA100109 (to J.C.). | ||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sy, SMH | en_HK |
dc.contributor.author | Huen, MSY | en_HK |
dc.contributor.author | Chen, J | en_HK |
dc.date.accessioned | 2010-09-17T10:10:31Z | - |
dc.date.available | 2010-09-17T10:10:31Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Journal Of Biological Chemistry, 2009, v. 284 n. 32, p. 21127-21131 | en_HK |
dc.identifier.issn | 0021-9258 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/90930 | - |
dc.description.abstract | PALB2 is an integral component of the BRCA complex important for recombinational DNA repair. However, exactly how this activity is regulated in vivo remains unexplored. Here we provide evidefice to show that MRG15 is a novel PALB2-associated protein that ensures regulated recombination events. We found that the direct interaction between MRG15 and PALB2 is mediated by an evolutionarily conserved region on PALB2. Intriguingly, although damage-induced RAD51 foci formation and mitomycin C sensitivity appeared normal in MRG15-binding defective PALB2 mutants, these cells exhibited a significant increase in gene conversion rates. Consistently, we found that abrogation of the PALB2-MRG15 interaction resulted in elevated sister chromatid exchange frequencies. Our results suggest that loss of the PALB2-ARG15 interaction relieved the cells with the suppression of sister chromatid exchange and therefore led to a hyper-recombination phenotype in the gene conversion assay. Together, our study indicated that although PALB2 is required for proficient homologous recombination, it could also govern the choice of templates used in homologous recombination repair. © 2009 by The American Society for Biochemistry and Molecular Biology, Inc. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_HK |
dc.relation.ispartof | Journal of Biological Chemistry | en_HK |
dc.subject | Chemicals And Cas Registry Numbers | en_HK |
dc.title | MRG15 is a novel PALB2-interacting factor involved in homologous recombination | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Huen, MSY:huen.michael@hku.hk | en_HK |
dc.identifier.authority | Huen, MSY=rp01336 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1074/jbc.C109.023937 | en_HK |
dc.identifier.pmid | 19553677 | - |
dc.identifier.pmcid | PMC2755835 | - |
dc.identifier.scopus | eid_2-s2.0-69249088889 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-69249088889&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 284 | en_HK |
dc.identifier.issue | 32 | en_HK |
dc.identifier.spage | 21127 | en_HK |
dc.identifier.epage | 21131 | en_HK |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.isi | WOS:000268564400005 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Sy, SMH=6602984466 | en_HK |
dc.identifier.scopusauthorid | Huen, MSY=23004751500 | en_HK |
dc.identifier.scopusauthorid | Chen, J=35261693300 | en_HK |
dc.identifier.issnl | 0021-9258 | - |