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- Publisher Website: 10.1021/jp807990g
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- PMID: 18956900
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Article: Hierarchical and helical self-assembly of ADP-ribosyl cyclase into large-scale protein microtubes
Title | Hierarchical and helical self-assembly of ADP-ribosyl cyclase into large-scale protein microtubes | ||||||||
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Authors | |||||||||
Issue Date | 2008 | ||||||||
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jpcbfk | ||||||||
Citation | Journal Of Physical Chemistry B, 2008, v. 112 n. 47, p. 14682-14686 How to Cite? | ||||||||
Abstract | Proteins are macromolecules with characteristic structures and biological functions. It is extremely challenging to obtain protein microtube structures through self-assembly as proteins are very complex and flexible. Here we present a strategy showing how a specific protein, ADP-ribosyl cyclase, helically self-assembles from monomers into hexagonal nanochains and further to highly ordered crystalline microtubes. The structures of protein nanochains and consequently self-assembled superlattice were determined by X-ray crystallography at 4.5 Å resolution and imaged by scanning electron microscopy. The protein initially forms into dimers that have a fixed size of 5.6 nm, and then, helically self-assembles into 35.6 nm long hexagonal nanochains. One such nanochain consists of six dimers (12 monomers) that stack in order by a pseudo P6 1 screw axis. Seven nanochains produce a series of large-scale assemblies, nanorods, forming the building blocks for microrods. A proposed aging process of microrods results in the formation of hollow microstructures. Synthesis and characterization of large scale self-assembled protein microtubes may pave a new pathway, capable of not only understanding the self-assembly dynamics of biological materials, but also directing design and fabrication of multifunctional nanobuilding blocks with particular applications in biomedical engineering. © 2008 American Chemical Society. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/91908 | ||||||||
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.760 | ||||||||
ISI Accession Number ID |
Funding Information: We are grateful to Dr. Marian Szebenyi for help in the indexing of diffraction data and Professor Dan Luo and Professor Younan Xia for critical reading of the manuscript. SEM images were collected at the Cornell Integrated Microscopy Center (CIMC) with help from Drs. Mandayarn Parthasarathy and Carole Daugherty. This work was supported by grants from the NIH to MacCHESS (RR01646) and H.C.L./ Q.H. (GM061568). The crystallographic data were collected at the Cornell High Energy Synchrotron Source (CHESS), which is supported by the NSF and NIH National Institute of General Medical Sciences under award DMR-0225180. | ||||||||
References |
DC Field | Value | Language |
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dc.contributor.author | Liu, Q | en_HK |
dc.contributor.author | Kriksunov, IA | en_HK |
dc.contributor.author | Wang, Z | en_HK |
dc.contributor.author | Graeff, R | en_HK |
dc.contributor.author | Lee, HC | en_HK |
dc.contributor.author | Hao, Q | en_HK |
dc.date.accessioned | 2010-09-17T10:30:12Z | - |
dc.date.available | 2010-09-17T10:30:12Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Journal Of Physical Chemistry B, 2008, v. 112 n. 47, p. 14682-14686 | en_HK |
dc.identifier.issn | 1520-6106 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/91908 | - |
dc.description.abstract | Proteins are macromolecules with characteristic structures and biological functions. It is extremely challenging to obtain protein microtube structures through self-assembly as proteins are very complex and flexible. Here we present a strategy showing how a specific protein, ADP-ribosyl cyclase, helically self-assembles from monomers into hexagonal nanochains and further to highly ordered crystalline microtubes. The structures of protein nanochains and consequently self-assembled superlattice were determined by X-ray crystallography at 4.5 Å resolution and imaged by scanning electron microscopy. The protein initially forms into dimers that have a fixed size of 5.6 nm, and then, helically self-assembles into 35.6 nm long hexagonal nanochains. One such nanochain consists of six dimers (12 monomers) that stack in order by a pseudo P6 1 screw axis. Seven nanochains produce a series of large-scale assemblies, nanorods, forming the building blocks for microrods. A proposed aging process of microrods results in the formation of hollow microstructures. Synthesis and characterization of large scale self-assembled protein microtubes may pave a new pathway, capable of not only understanding the self-assembly dynamics of biological materials, but also directing design and fabrication of multifunctional nanobuilding blocks with particular applications in biomedical engineering. © 2008 American Chemical Society. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jpcbfk | en_HK |
dc.relation.ispartof | Journal of Physical Chemistry B | en_HK |
dc.title | Hierarchical and helical self-assembly of ADP-ribosyl cyclase into large-scale protein microtubes | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Graeff, R: graeffr@hku.hk | en_HK |
dc.identifier.email | Lee, HC: leehc@hku.hk | en_HK |
dc.identifier.email | Hao, Q: qhao@hku.hk | en_HK |
dc.identifier.authority | Graeff, R=rp01464 | en_HK |
dc.identifier.authority | Lee, HC=rp00545 | en_HK |
dc.identifier.authority | Hao, Q=rp01332 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1021/jp807990g | en_HK |
dc.identifier.pmid | 18956900 | - |
dc.identifier.scopus | eid_2-s2.0-57449083063 | en_HK |
dc.identifier.hkuros | 154345 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-57449083063&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 112 | en_HK |
dc.identifier.issue | 47 | en_HK |
dc.identifier.spage | 14682 | en_HK |
dc.identifier.epage | 14686 | en_HK |
dc.identifier.isi | WOS:000261056400003 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Liu, Q=35215401600 | en_HK |
dc.identifier.scopusauthorid | Kriksunov, IA=6507909504 | en_HK |
dc.identifier.scopusauthorid | Wang, Z=36543473300 | en_HK |
dc.identifier.scopusauthorid | Graeff, R=7003614053 | en_HK |
dc.identifier.scopusauthorid | Lee, HC=26642959100 | en_HK |
dc.identifier.scopusauthorid | Hao, Q=7102508868 | en_HK |
dc.identifier.issnl | 1520-5207 | - |