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- Scopus: eid_2-s2.0-0031857928
- PMID: 9655690
- WOS: WOS:000074725200014
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Article: Secretory effects of ATP on nontransformed dog pancreatic duct epithelial cells
Title | Secretory effects of ATP on nontransformed dog pancreatic duct epithelial cells |
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Authors | |
Keywords | Adenosine 5'-triphosphate Chloride channels Cystic fibrosis Mucin Potassium channels Short-circuit current |
Issue Date | 1998 |
Publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/ |
Citation | American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1998, v. 275 n. 1 38-1, p. G104-G11 How to Cite? |
Abstract | Extracellular triphosphate nucleotides, such as ATP, may regulate various cellular functions through specific cell surface receptors. We examine in this report the different secretory effects of ATP and analogs on nontransformed dog pancreatic duct epithelial cells (PDEC). We observed that 1) ATP, UTP, adenosine 5'-O-(3-thiotriphosphate), and, to a lesser extent, β,γ-methylene-ATP, but not adenosine, stimulated 125I- efflux from PDEC, suggesting a primary role for P(2Y2) receptors, 2) ATP-stimulated 125I- efflux was inhibited by 5-nitro-2-(3-phenylpropylamino)benzoic acid, diphenylamine-2-carboxylate, and DIDS, suggesting mediation through Ca 2+- activated Cl - channels, 3) ATP stimulated an 86Rb + efflux sensitive to BaCl 2 and charybdotoxin, thus likely occurring through Ca 2+-activated K + channels, 4) serosal or luminal addition of UTP activated apical Cl - conductance and basolateral K + conductance when nystatin-permeabilized PDEC were studied in an Ussing chamber, suggesting the expression of P(2Y2) receptors on both sides of the cell, 5) ATP stimulated mucin secretion, and 6) ATP increases intracellular Ca 2+ concentration ([Ca 2+](i)). In conclusion, ATP and UTP interact with P(2Y2) receptors on nontransformed PDEC to increase [Ca 2+](i), stimulate mucin secretion, and activate ion conductances; these findings have implications for pancreatic exocrine function in both health and disease, such as cystic fibrosis. |
Persistent Identifier | http://hdl.handle.net/10722/92484 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 1.460 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nguyen, TD | en_HK |
dc.contributor.author | Moody, MW | en_HK |
dc.contributor.author | Savard, CE | en_HK |
dc.contributor.author | Lee, SP | en_HK |
dc.date.accessioned | 2010-09-17T10:47:40Z | - |
dc.date.available | 2010-09-17T10:47:40Z | - |
dc.date.issued | 1998 | en_HK |
dc.identifier.citation | American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1998, v. 275 n. 1 38-1, p. G104-G11 | en_HK |
dc.identifier.issn | 0193-1857 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/92484 | - |
dc.description.abstract | Extracellular triphosphate nucleotides, such as ATP, may regulate various cellular functions through specific cell surface receptors. We examine in this report the different secretory effects of ATP and analogs on nontransformed dog pancreatic duct epithelial cells (PDEC). We observed that 1) ATP, UTP, adenosine 5'-O-(3-thiotriphosphate), and, to a lesser extent, β,γ-methylene-ATP, but not adenosine, stimulated 125I- efflux from PDEC, suggesting a primary role for P(2Y2) receptors, 2) ATP-stimulated 125I- efflux was inhibited by 5-nitro-2-(3-phenylpropylamino)benzoic acid, diphenylamine-2-carboxylate, and DIDS, suggesting mediation through Ca 2+- activated Cl - channels, 3) ATP stimulated an 86Rb + efflux sensitive to BaCl 2 and charybdotoxin, thus likely occurring through Ca 2+-activated K + channels, 4) serosal or luminal addition of UTP activated apical Cl - conductance and basolateral K + conductance when nystatin-permeabilized PDEC were studied in an Ussing chamber, suggesting the expression of P(2Y2) receptors on both sides of the cell, 5) ATP stimulated mucin secretion, and 6) ATP increases intracellular Ca 2+ concentration ([Ca 2+](i)). In conclusion, ATP and UTP interact with P(2Y2) receptors on nontransformed PDEC to increase [Ca 2+](i), stimulate mucin secretion, and activate ion conductances; these findings have implications for pancreatic exocrine function in both health and disease, such as cystic fibrosis. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/ | en_HK |
dc.relation.ispartof | American Journal of Physiology - Gastrointestinal and Liver Physiology | en_HK |
dc.subject | Adenosine 5'-triphosphate | en_HK |
dc.subject | Chloride channels | en_HK |
dc.subject | Cystic fibrosis | en_HK |
dc.subject | Mucin | en_HK |
dc.subject | Potassium channels | en_HK |
dc.subject | Short-circuit current | en_HK |
dc.title | Secretory effects of ATP on nontransformed dog pancreatic duct epithelial cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lee, SP: sumlee@hku.hk | en_HK |
dc.identifier.authority | Lee, SP=rp01351 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.pmid | 9655690 | - |
dc.identifier.scopus | eid_2-s2.0-0031857928 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0031857928&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 275 | en_HK |
dc.identifier.issue | 1 38-1 | en_HK |
dc.identifier.spage | G104 | en_HK |
dc.identifier.epage | G11 | en_HK |
dc.identifier.isi | WOS:000074725200014 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Nguyen, TD=35546959700 | en_HK |
dc.identifier.scopusauthorid | Moody, MW=7102854884 | en_HK |
dc.identifier.scopusauthorid | Savard, CE=6701738621 | en_HK |
dc.identifier.scopusauthorid | Lee, SP=7601417497 | en_HK |
dc.identifier.issnl | 0193-1857 | - |