Knocking down interferon induced transmembrane protein 1 (IFITM1) inhibited cell proliferation and invasion in glioma cells

Abstract

Here we investigated the role of Interferon Induced Transmembrane Protein 1 (IFITM1) in glioma carcinogenesis. We determined the expression levels of IFITM1 in different glioma cell lines, examined its role in cell proliferation/cell migration & invasion, and the corresponding molecular mechanism in glioma cells. Our results showed that IFITM1 mRNA level was elevated in four of five widely used glioma cell lines and positively correlated with their tumorigenic abilities. Suppression of IFITM1 by siIFITM1 inhibited the proliferation of U-373 MG and U-87 MG glioma cells lines in a time dependent manner. The growth inhibition effect of siIFITM1 was mediated not by apoptosis but G1 to S phase cell cycle arrest, which are mediated via decreased G1/S-specific cylin-D1, cyclin-dependent kinase 2 (CDK2) and increased cyclin-dependent kinase inhibitor 1B (p27kip1). Furthermore, IFITM1 knockdown significantly inhibit the migration and invasion of U-373 MG and U-87 MG cells, which was mediated by decreased MMP9 expression and activity. Taken together, these results suggested IFITM1 might be a potential candidate oncogene in glioma.