File Download
There are no files associated with this item.
Supplementary
-
Citations:
- PubMed Central: 0
- Appears in Collections:
Conference Paper: Immunohistochemical markers, are we having too many choices?
Title | Immunohistochemical markers, are we having too many choices? |
---|---|
Authors | |
Issue Date | 2007 |
Publisher | Malaysian Society of Pathologists |
Citation | The 24th World Congress of Pathology and Laboratory Medicine, Kuala Lumpur, Malaysia, 20-24 August 2007. In The Malaysian Journal of Pathology, 2007, v. 29 n. Supplement A, p. 182 How to Cite? |
Abstract | As more and more antibodies are made available for immunohistochemical studies, pathologists are
increasing being challenged to make a rational choice from multiple antibodies all of which are reputably
suited for the same or similar diagnostic application. A recent review was conducted into the practice
of a group of histopathologists working in a general teaching hospital in how they had used the three
antibodies, namely smooth muscle actin, calponin and p63, each of which could be used as a marker for
myoepithelial differentiation. The findings are presented and discussed. Immunohistochemical studies
recently carried out in the histopathology laboratory of the Queen Mary Hospital, Hong Kong were
recorded in a computerized database. The database was searched for all stains performed for actin,
calponin, and p63 from Jan 2005 to the end of April 2007. Parameters selected were the accession
number complete with the tissue block number, antibodies stained and the pathologist ordering the
stains. A total of 1148 blocks were stained for actin (n=803), calponin (n=127), and p63 (n=574).
Pathologists varied widely in their choice of applying these antibodies. While it is uncommon to use
all 3 antibodies on a single block (n=38), one pathologist alone was responsible for 76% (n=29) of this
usage. Slightly more blocks were stained for p63 alone (n=291) then those stained in combinations
with actin and/or calponin (n=283). Significant variation in the use of the 3 antibodies was observed
among a group of 24 pathologists working in a single public institution. Stereotypic patterns of
applying immunohistochemical stains may result in excessive procedures. A system to monitor and
provide timely feedback on the usage of immunohistochemical markers with the adoption of common
protocols would effect cost cuts and more efficient running of laboratory services. |
Persistent Identifier | http://hdl.handle.net/10722/104435 |
ISSN | 2023 Impact Factor: 0.6 2023 SCImago Journal Rankings: 0.271 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, KW | en_HK |
dc.contributor.author | Chan, GSW | en_HK |
dc.date.accessioned | 2010-09-25T21:52:59Z | - |
dc.date.available | 2010-09-25T21:52:59Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | The 24th World Congress of Pathology and Laboratory Medicine, Kuala Lumpur, Malaysia, 20-24 August 2007. In The Malaysian Journal of Pathology, 2007, v. 29 n. Supplement A, p. 182 | - |
dc.identifier.issn | 0126-8635 | - |
dc.identifier.uri | http://hdl.handle.net/10722/104435 | - |
dc.description.abstract | As more and more antibodies are made available for immunohistochemical studies, pathologists are increasing being challenged to make a rational choice from multiple antibodies all of which are reputably suited for the same or similar diagnostic application. A recent review was conducted into the practice of a group of histopathologists working in a general teaching hospital in how they had used the three antibodies, namely smooth muscle actin, calponin and p63, each of which could be used as a marker for myoepithelial differentiation. The findings are presented and discussed. Immunohistochemical studies recently carried out in the histopathology laboratory of the Queen Mary Hospital, Hong Kong were recorded in a computerized database. The database was searched for all stains performed for actin, calponin, and p63 from Jan 2005 to the end of April 2007. Parameters selected were the accession number complete with the tissue block number, antibodies stained and the pathologist ordering the stains. A total of 1148 blocks were stained for actin (n=803), calponin (n=127), and p63 (n=574). Pathologists varied widely in their choice of applying these antibodies. While it is uncommon to use all 3 antibodies on a single block (n=38), one pathologist alone was responsible for 76% (n=29) of this usage. Slightly more blocks were stained for p63 alone (n=291) then those stained in combinations with actin and/or calponin (n=283). Significant variation in the use of the 3 antibodies was observed among a group of 24 pathologists working in a single public institution. Stereotypic patterns of applying immunohistochemical stains may result in excessive procedures. A system to monitor and provide timely feedback on the usage of immunohistochemical markers with the adoption of common protocols would effect cost cuts and more efficient running of laboratory services. | - |
dc.language | eng | en_HK |
dc.publisher | Malaysian Society of Pathologists | - |
dc.relation.ispartof | The Malaysian Journal of Pathology | en_HK |
dc.title | Immunohistochemical markers, are we having too many choices? | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.email | Chan, KW: hrmtckw@hku.hk | en_HK |
dc.identifier.email | Chan, GSW: chanswg@HKUCC.hku.hk | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.pmid | 18426038 | - |
dc.identifier.hkuros | 150614 | en_HK |
dc.identifier.issnl | 0126-8635 | - |