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Article: In vivo MRI of endogenous stem/progenitor cell migration from subventricular zone in normal and injured developing brains
Title | In vivo MRI of endogenous stem/progenitor cell migration from subventricular zone in normal and injured developing brains | ||||||
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Authors | |||||||
Keywords | Developmental brain Hypoxia Iron oxide particles Ischemia Migration MRI Neural stem/progenitor cells | ||||||
Issue Date | 2009 | ||||||
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ynimg | ||||||
Citation | Neuroimage, 2009, v. 48 n. 2, p. 319-328 How to Cite? | ||||||
Abstract | Understanding the alterations of migratory activities of the endogenous neural stem/progenitor cells (NSPs) in injured developing brains is becoming increasingly imperative for curative reasons. In this study, 10-day-old neonatal rats with and without hypoxic-ischemic (HI) insult at postnatal day 7 were injected intraventricularly with micron-sized iron oxide particles (MPIOs), followed by serial high-resolution MRI at 7 T for 2 weeks. MRI findings were correlated to the histological analysis using iron staining and several immunohistochemical double staining. The results indicated that in normal and HI-injured brains the NSPs from the subventricular zone (SVZ) were labeled by MPIOs, and migrated as newly created cells (iron+/BrdU+), neuroblasts (iron+/nestin+), astrocytes or astrocytes-like progenitor cells (iron+/GFAP+), and mature neurons (iron+/NeuN+). In normal brains, the endogenous NSPs mainly exhibited a tangential pattern in both rostral and caudal directions. The NSP radial migratory pattern could be observed in some rats. In the HI-injured brains during the same developmental period, the NSPs mainly migrated towards the HI lesion sites. The tangential, rostrocaudal migrations could be observed but impaired. These findings suggest that the NSP migratory pathways in SVZ change in response to the HI insult, likely due to the self-repairing efforts known in the neonatal brains. The MRI approach demonstrated here is potentially applicable to the in vivo and longitudinal study of NSP cell activities in developing brains under normal and pathological conditions and in therapeutic interventions. © 2009 Elsevier Inc. All rights reserved. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/129231 | ||||||
ISSN | 2023 Impact Factor: 4.7 2023 SCImago Journal Rankings: 2.436 | ||||||
ISI Accession Number ID |
Funding Information: This work was supported in part by research grants from the Hong Kong Research Grant Council (HKU 7793/08M) and the Chinese National Science Foundation Council (30770673). | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, J | en_HK |
dc.contributor.author | Liu, J | en_HK |
dc.contributor.author | Niu, G | en_HK |
dc.contributor.author | Chan, KC | en_HK |
dc.contributor.author | Wang, R | en_HK |
dc.contributor.author | Liu, Y | en_HK |
dc.contributor.author | Wu, EX | en_HK |
dc.date.accessioned | 2010-12-23T08:33:47Z | - |
dc.date.available | 2010-12-23T08:33:47Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Neuroimage, 2009, v. 48 n. 2, p. 319-328 | en_HK |
dc.identifier.issn | 1053-8119 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/129231 | - |
dc.description.abstract | Understanding the alterations of migratory activities of the endogenous neural stem/progenitor cells (NSPs) in injured developing brains is becoming increasingly imperative for curative reasons. In this study, 10-day-old neonatal rats with and without hypoxic-ischemic (HI) insult at postnatal day 7 were injected intraventricularly with micron-sized iron oxide particles (MPIOs), followed by serial high-resolution MRI at 7 T for 2 weeks. MRI findings were correlated to the histological analysis using iron staining and several immunohistochemical double staining. The results indicated that in normal and HI-injured brains the NSPs from the subventricular zone (SVZ) were labeled by MPIOs, and migrated as newly created cells (iron+/BrdU+), neuroblasts (iron+/nestin+), astrocytes or astrocytes-like progenitor cells (iron+/GFAP+), and mature neurons (iron+/NeuN+). In normal brains, the endogenous NSPs mainly exhibited a tangential pattern in both rostral and caudal directions. The NSP radial migratory pattern could be observed in some rats. In the HI-injured brains during the same developmental period, the NSPs mainly migrated towards the HI lesion sites. The tangential, rostrocaudal migrations could be observed but impaired. These findings suggest that the NSP migratory pathways in SVZ change in response to the HI insult, likely due to the self-repairing efforts known in the neonatal brains. The MRI approach demonstrated here is potentially applicable to the in vivo and longitudinal study of NSP cell activities in developing brains under normal and pathological conditions and in therapeutic interventions. © 2009 Elsevier Inc. All rights reserved. | en_HK |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ynimg | en_HK |
dc.relation.ispartof | NeuroImage | en_HK |
dc.subject | Developmental brain | - |
dc.subject | Hypoxia | - |
dc.subject | Iron oxide particles | - |
dc.subject | Ischemia | - |
dc.subject | Migration | - |
dc.subject | MRI | - |
dc.subject | Neural stem/progenitor cells | - |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Astrocytes - physiology | en_HK |
dc.subject.mesh | Brain - growth & development - physiology - physiopathology | en_HK |
dc.subject.mesh | Bromodeoxyuridine | en_HK |
dc.subject.mesh | Cell Movement - physiology | en_HK |
dc.subject.mesh | Ferric Compounds | en_HK |
dc.subject.mesh | Glial Fibrillary Acidic Protein - metabolism | en_HK |
dc.subject.mesh | Hypoxia-Ischemia, Brain - physiopathology | en_HK |
dc.subject.mesh | Immunohistochemistry | en_HK |
dc.subject.mesh | Intermediate Filament Proteins - metabolism | en_HK |
dc.subject.mesh | Iron - metabolism | en_HK |
dc.subject.mesh | Magnetic Resonance Imaging | en_HK |
dc.subject.mesh | Nerve Tissue Proteins - metabolism | en_HK |
dc.subject.mesh | Neurons - physiology | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.subject.mesh | Stem Cell Niche - physiology | en_HK |
dc.subject.mesh | Stem Cells - physiology | en_HK |
dc.subject.mesh | Time Factors | en_HK |
dc.title | In vivo MRI of endogenous stem/progenitor cell migration from subventricular zone in normal and injured developing brains | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Wu, EX:ewu1@hkucc.hku.hk | en_HK |
dc.identifier.authority | Wu, EX=rp00193 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.neuroimage.2009.06.075 | en_HK |
dc.identifier.pmid | 19591946 | - |
dc.identifier.scopus | eid_2-s2.0-68949184821 | en_HK |
dc.identifier.hkuros | 177186 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-68949184821&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 48 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 319 | en_HK |
dc.identifier.epage | 328 | en_HK |
dc.identifier.isi | WOS:000274723900002 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Yang, J=10041733800 | en_HK |
dc.identifier.scopusauthorid | Liu, J=8345637700 | en_HK |
dc.identifier.scopusauthorid | Niu, G=35273273000 | en_HK |
dc.identifier.scopusauthorid | Chan, KC=34968940300 | en_HK |
dc.identifier.scopusauthorid | Wang, R=36071507000 | en_HK |
dc.identifier.scopusauthorid | Liu, Y=35196202900 | en_HK |
dc.identifier.scopusauthorid | Wu, EX=7202128034 | en_HK |
dc.identifier.citeulike | 5220206 | - |
dc.identifier.issnl | 1053-8119 | - |