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Article: Differential actions of glycodelin-A on Th-1 and Th-2 cells: A paracrine mechanism that could produce the Th-2 dominant environment during pregnancy
Title | Differential actions of glycodelin-A on Th-1 and Th-2 cells: A paracrine mechanism that could produce the Th-2 dominant environment during pregnancy | ||||
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Authors | |||||
Keywords | cell death Fas glycodelin T-helper cells Th-1/Th-2 | ||||
Issue Date | 2011 | ||||
Publisher | Oxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/ | ||||
Citation | Human Reproduction, 2011, v. 26 n. 3, p. 517-526 How to Cite? | ||||
Abstract | Background: The maternalfetal interface has a unique immunological response towards the implanting placenta. It is generally accepted that a T-helper type-2 (Th-2) cytokine prevailing environment is important in pregnancy. The proportion of Th-2 cells in the peripheral blood and decidua is significantly higher in pregnant women in the first trimester than in non-pregnant women. Glycodelin-A (GdA) is a major endocrine-regulated decidual glycoprotein thought to be related to fetomaternal defence. Yet the relationship between its immunoregulatory activities and the shift towards Th-2 cytokine profile during pregnancy is unclear. Methods GdA was immunoaffinity purified from human amniotic fluid. T-helper, T-helper type-1 (Th-1) and Th-2 cells were isolated from the peripheral blood. The viability of these cells was studied by XTT assay. Immunophenotyping of CD4/CD294, cell death and GdA-binding were determined by flow cytometry. The mRNA expression, surface expression and secretion of Fas/Fas ligand (FasL) were determined by quantitative polymerase chain reaction, flow cytometry and ELISA, respectively. The activities of caspase-3, -8 and -9 were measured. The phosphorylation of extracellular signal-regulated kinases (ERK), p38 and, c-Jun N-terminal kinase was determined by western blotting. Results Although GdA bound to both Th-1 and Th-2 cells, it had differential actions on the two cell-types. GdA induced cell death of the Th-1 cells but not the Th-2 cells. The cell death was mediated through activation of caspase -3, -8 and -9 activities. GdA up-regulated the expression of Fas and inhibited ERK activation in the Th-1 cells, which might enhance the vulnerability of the cells to cell death caused by a trophoblast-derived FasL. Conclusions The data suggest that GdA could be an endometrial factor that contributes to the Th-2/Th-1 shift during pregnancy. © 2011 The Author. | ||||
Persistent Identifier | http://hdl.handle.net/10722/135025 | ||||
ISSN | 2023 Impact Factor: 6.0 2023 SCImago Journal Rankings: 1.852 | ||||
ISI Accession Number ID |
Funding Information: This study is supported in part by the Helsinki University Central Hospital Research Fund. | ||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, CL | en_HK |
dc.contributor.author | Chiu, PCN | en_HK |
dc.contributor.author | Lam, KKW | en_HK |
dc.contributor.author | Siu, SO | en_HK |
dc.contributor.author | Chu, IK | en_HK |
dc.contributor.author | Koistinen, R | en_HK |
dc.contributor.author | Koistinen, H | en_HK |
dc.contributor.author | Seppl, M | en_HK |
dc.contributor.author | Lee, KF | en_HK |
dc.contributor.author | Yeung, WSB | en_HK |
dc.date.accessioned | 2011-07-27T01:26:14Z | - |
dc.date.available | 2011-07-27T01:26:14Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Human Reproduction, 2011, v. 26 n. 3, p. 517-526 | en_HK |
dc.identifier.issn | 0268-1161 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/135025 | - |
dc.description.abstract | Background: The maternalfetal interface has a unique immunological response towards the implanting placenta. It is generally accepted that a T-helper type-2 (Th-2) cytokine prevailing environment is important in pregnancy. The proportion of Th-2 cells in the peripheral blood and decidua is significantly higher in pregnant women in the first trimester than in non-pregnant women. Glycodelin-A (GdA) is a major endocrine-regulated decidual glycoprotein thought to be related to fetomaternal defence. Yet the relationship between its immunoregulatory activities and the shift towards Th-2 cytokine profile during pregnancy is unclear. Methods GdA was immunoaffinity purified from human amniotic fluid. T-helper, T-helper type-1 (Th-1) and Th-2 cells were isolated from the peripheral blood. The viability of these cells was studied by XTT assay. Immunophenotyping of CD4/CD294, cell death and GdA-binding were determined by flow cytometry. The mRNA expression, surface expression and secretion of Fas/Fas ligand (FasL) were determined by quantitative polymerase chain reaction, flow cytometry and ELISA, respectively. The activities of caspase-3, -8 and -9 were measured. The phosphorylation of extracellular signal-regulated kinases (ERK), p38 and, c-Jun N-terminal kinase was determined by western blotting. Results Although GdA bound to both Th-1 and Th-2 cells, it had differential actions on the two cell-types. GdA induced cell death of the Th-1 cells but not the Th-2 cells. The cell death was mediated through activation of caspase -3, -8 and -9 activities. GdA up-regulated the expression of Fas and inhibited ERK activation in the Th-1 cells, which might enhance the vulnerability of the cells to cell death caused by a trophoblast-derived FasL. Conclusions The data suggest that GdA could be an endometrial factor that contributes to the Th-2/Th-1 shift during pregnancy. © 2011 The Author. | en_HK |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/ | en_HK |
dc.relation.ispartof | Human Reproduction | en_HK |
dc.rights | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 2011, v. 26 n. 3, p. 517-526 is available online at: http://humrep.oxfordjournals.org/content/26/3/517 | - |
dc.subject | cell death | en_HK |
dc.subject | Fas | en_HK |
dc.subject | glycodelin | en_HK |
dc.subject | T-helper cells | en_HK |
dc.subject | Th-1/Th-2 | en_HK |
dc.subject.mesh | Amniotic Fluid - chemistry | - |
dc.subject.mesh | Glycoproteins - chemistry - isolation and purification - metabolism | - |
dc.subject.mesh | Pregnancy Proteins - chemistry - isolation and purification - metabolism | - |
dc.subject.mesh | Th1 Cells - immunology - metabolism - secretion | - |
dc.subject.mesh | Th2 Cells - immunology - metabolism - secretion | - |
dc.title | Differential actions of glycodelin-A on Th-1 and Th-2 cells: A paracrine mechanism that could produce the Th-2 dominant environment during pregnancy | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=26&issue=3&spage=517&epage=526&date=2011&atitle=Differential+actions+of+glycodelin-A+on+Th-1+and+Th-2+cells:+a+paracrine+mechanism+that+could+produce+The+Th-2+dominant+environment+during+pregnancy | - |
dc.identifier.email | Chiu, PCN:pchiucn@hku.hk | en_HK |
dc.identifier.email | Chu, IK:ivankchu@hku.hk | en_HK |
dc.identifier.email | Lee, KF:ckflee@hku.hk | en_HK |
dc.identifier.email | Yeung, WSB:wsbyeung@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chiu, PCN=rp00424 | en_HK |
dc.identifier.authority | Chu, IK=rp00683 | en_HK |
dc.identifier.authority | Lee, KF=rp00458 | en_HK |
dc.identifier.authority | Yeung, WSB=rp00331 | en_HK |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1093/humrep/deq381 | en_HK |
dc.identifier.pmid | 21227941 | - |
dc.identifier.scopus | eid_2-s2.0-79951564852 | en_HK |
dc.identifier.hkuros | 186183 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79951564852&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 26 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 517 | en_HK |
dc.identifier.epage | 526 | en_HK |
dc.identifier.isi | WOS:000287252800003 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Lee, CL=9277221100 | en_HK |
dc.identifier.scopusauthorid | Chiu, PCN=25959969200 | en_HK |
dc.identifier.scopusauthorid | Lam, KKW=25637362300 | en_HK |
dc.identifier.scopusauthorid | Siu, SO=55041035300 | en_HK |
dc.identifier.scopusauthorid | Chu, IK=7103327484 | en_HK |
dc.identifier.scopusauthorid | Koistinen, R=7006574669 | en_HK |
dc.identifier.scopusauthorid | Koistinen, H=7003612125 | en_HK |
dc.identifier.scopusauthorid | Seppl, M=36970056200 | en_HK |
dc.identifier.scopusauthorid | Lee, KF=26643097500 | en_HK |
dc.identifier.scopusauthorid | Yeung, WSB=7102370745 | en_HK |
dc.identifier.citeulike | 8656772 | - |
dc.identifier.issnl | 0268-1161 | - |