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- Publisher Website: 10.1007/978-1-62703-125-7_21
- Scopus: eid_2-s2.0-84870537180
- PMID: 23097117
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Article: Human female reproductive tract epithelial cell culture
Title | Human female reproductive tract epithelial cell culture |
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Authors | |
Keywords | Endometrium In vitro culture Ovary Oviduct Reproduction |
Issue Date | 2013 |
Publisher | Humana Press, Inc. |
Citation | Methods in Molecular Biology, 2013, v. 945, p. 347-363 How to Cite? |
Abstract | The female reproductive system is a complex system. Epithelia of the female reproductive system including the ovaries, the oviduct, and the uterus are important sites for follicular development, ovulation, fertilization, implantation, and embryo development. They are also able to synthesize and secrete various hormones, growth factors, and cytokines, which are essential to women's health, sexuality, and reproduction. Conversely, their dysfunction has been implicated in disorders such as infertility, endometriosis, and many other gynecological diseases, as well as cancer. In this chapter, we describe detailed procedures for establishing and maintaining primary cultures of human ovarian surface epithelium, oviductal epithelium, and endometrium. We also provide protocols for cell immortalization, clonal isolation, and in coculture with stromal cells. These cultures can be useful models for investigating the molecular and cellular functions of these epithelia in both normal and pathological states. |
Persistent Identifier | http://hdl.handle.net/10722/136662 |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Chan, RWS | en_US |
dc.contributor.author | Mak, ASC | - |
dc.contributor.author | Yeung, WSB | - |
dc.contributor.author | Lee, CKF | - |
dc.contributor.author | Cheung, ANY | - |
dc.contributor.author | Ngan, HYS | - |
dc.contributor.author | Wong, AST | - |
dc.date.accessioned | 2011-07-27T02:27:17Z | - |
dc.date.available | 2011-07-27T02:27:17Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Methods in Molecular Biology, 2013, v. 945, p. 347-363 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | http://hdl.handle.net/10722/136662 | - |
dc.description.abstract | The female reproductive system is a complex system. Epithelia of the female reproductive system including the ovaries, the oviduct, and the uterus are important sites for follicular development, ovulation, fertilization, implantation, and embryo development. They are also able to synthesize and secrete various hormones, growth factors, and cytokines, which are essential to women's health, sexuality, and reproduction. Conversely, their dysfunction has been implicated in disorders such as infertility, endometriosis, and many other gynecological diseases, as well as cancer. In this chapter, we describe detailed procedures for establishing and maintaining primary cultures of human ovarian surface epithelium, oviductal epithelium, and endometrium. We also provide protocols for cell immortalization, clonal isolation, and in coculture with stromal cells. These cultures can be useful models for investigating the molecular and cellular functions of these epithelia in both normal and pathological states. | - |
dc.language | eng | en_US |
dc.publisher | Humana Press, Inc. | en_US |
dc.relation.ispartof | Methods in Molecular Biology | - |
dc.rights | The original publication is available at www.springerlink.com | - |
dc.subject | Endometrium | - |
dc.subject | In vitro culture | - |
dc.subject | Ovary | - |
dc.subject | Oviduct | - |
dc.subject | Reproduction | - |
dc.subject.mesh | Antigens, Polyomavirus Transforming - genetics | - |
dc.subject.mesh | Cell Culture Techniques - methods | - |
dc.subject.mesh | Cell Proliferation | - |
dc.subject.mesh | Epithelial Cells - cytology - metabolism | - |
dc.subject.mesh | Genitalia, Female - cytology | - |
dc.title | Human female reproductive tract epithelial cell culture | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chan, RWS: rwschan@hku.hk | en_US |
dc.identifier.email | Mak, ASC: ascmak@hkucc.hku.hk | - |
dc.identifier.email | Yeung, WSB: wsbyeung@hkucc.hku.hk | - |
dc.identifier.email | Lee, CKF: ckflee@hkucc.hku.hk | - |
dc.identifier.email | Cheung, ANY: anycheun@hkucc.hku.hk | - |
dc.identifier.email | Ngan, HYS: hysngan@hkucc.hku.hk | - |
dc.identifier.email | Wong, AST: awong1@hku.hk | - |
dc.identifier.authority | Yeung, WSB=rp00331 | en_US |
dc.identifier.doi | 10.1007/978-1-62703-125-7_21 | - |
dc.identifier.pmid | 23097117 | - |
dc.identifier.scopus | eid_2-s2.0-84870537180 | - |
dc.identifier.hkuros | 188972 | en_US |
dc.identifier.hkuros | 205904 | - |
dc.identifier.hkuros | 221091 | - |
dc.identifier.hkuros | 238628 | - |
dc.identifier.volume | 945 | - |
dc.identifier.spage | 347 | - |
dc.identifier.epage | 363 | en_US |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1064-3745 | - |