Comparative genomic hybridization for the profile of chromosomal imbalances in cardia adenocarcinoma and metastatic lymph node lesions

Abstract

AIM: To profile the chromosomal imbalances in gastric cardia adenocarcinoma (GCA) and the metastatic lymph node (LN) lesions from the high incidence area of esophageal squamous cell carcinoma/GCA in Henan province, and to provide the molecular basis for identifying the related genes for GCA and GCA lymph nodes metastasis. METHODS: Chromosomal imbalances were studied in 30 GCA resection specimens and 7 metastatic LN lesions by comparative genomic hybridization (CGH). RESULTS: In 30 GCA specimens, a total of 126 gains and 117 losses were found, and the average gain and loss was 4.2 and 3.9, respectively. The chromosomal gain most frequently identified in GCA was on 20q (43%), and others were on 6q (40%), 8q (37%), 6p (33%), 7q (33%), 1q (30%), 7p (27%), 13q (23%), 11q (20%) and 5p (20%) respectively. Losses were observed predominantly on 17p (57%), 19q (50%), 1p (47%), 3p (30%) and 4p (20%). In 7 LN lesions, a total of 75 gains and 58 losses were found, and the average gain and loss were 10.7 and 8.3, respectively. Gains were detected on chromosome 8q (5/ 7), 20 (4/7) and 1q, 2p, 3q, 6, 7, 11p11-q13, 11q14-q23, 13 (3/7 each), and deletions on chromosome 9p13-qter (4/7), 4 (3/7) and 1pter-1q33, 7p12-qter, 16p, 17p, 18 (2/7 each). CONCLUSION: The gains of 20q, 6q, 8q, 7p, 6p, 7q, 1q, 13q, 11q, 5p and losses of 17p, 19q, 1p, 3p, 4p are specifically implicated in GCA, and gains of 8q, 20q and losses of 9p, 4pq may be associated with lymph node metastasis.