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- Publisher Website: 10.1038/emboj.2013.66
- Scopus: eid_2-s2.0-84876419201
- PMID: 23531880
- WOS: WOS:000317748400011
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Article: YY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locus
Title | YY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locus |
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Authors | |
Keywords | B-Cell Development Immunoglobulin Polycomb Rearrangement Yy1 |
Issue Date | 2013 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.html |
Citation | Embo Journal, 2013, v. 32 n. 8, p. 1168-1182 How to Cite? |
Abstract | Conditional knock-out (KO) of Polycomb Group (PcG) protein YY1 results in pro-B cell arrest and reduced immunoglobulin locus contraction needed for distal variable gene rearrangement. The mechanisms that control these crucial functions are unknown. We deleted the 25 amino-acid YY1 REPO domain necessary for YY1 PcG function, and used this mutant (YY1ΔREPO), to transduce bone marrow from YY1 conditional KO mice. While wild-type YY1 rescued B-cell development, YY1ΔREPO failed to rescue the B-cell lineage yielding reduced numbers of B lineage cells. Although the IgH rearrangement pattern was normal, there was a selective impact at the Igκ locus that showed a dramatic skewing of the expressed Igκ repertoire. We found that the REPO domain interacts with proteins from the condensin and cohesin complexes, and that YY1, EZH2 and condensin proteins co-localize at numerous sites across the Ig kappa locus. Knock-down of a condensin subunit protein or YY1 reduced rearrangement of Igκ Vκ genes suggesting a direct role for YY1-condensin complexes in Igκ locus structure and rearrangement. © 2013 European Molecular Biology Organization. |
Persistent Identifier | http://hdl.handle.net/10722/184314 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 5.489 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Pan, X | en_US |
dc.contributor.author | Papasani, M | en_US |
dc.contributor.author | Hao, Y | en_US |
dc.contributor.author | Calamito, M | en_US |
dc.contributor.author | Wei, F | en_US |
dc.contributor.author | Quinn, WJ | en_US |
dc.contributor.author | Basu, A | en_US |
dc.contributor.author | Wang, J | en_US |
dc.contributor.author | Hodawadekar, S | en_US |
dc.contributor.author | Zaprazna, K | en_US |
dc.contributor.author | Liu, H | en_US |
dc.contributor.author | Shi, Y | en_US |
dc.contributor.author | Allman, D | en_US |
dc.contributor.author | Cancro, M | en_US |
dc.contributor.author | Atchison, ML | en_US |
dc.date.accessioned | 2013-07-10T06:23:39Z | - |
dc.date.available | 2013-07-10T06:23:39Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Embo Journal, 2013, v. 32 n. 8, p. 1168-1182 | en_US |
dc.identifier.issn | 0261-4189 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/184314 | - |
dc.description.abstract | Conditional knock-out (KO) of Polycomb Group (PcG) protein YY1 results in pro-B cell arrest and reduced immunoglobulin locus contraction needed for distal variable gene rearrangement. The mechanisms that control these crucial functions are unknown. We deleted the 25 amino-acid YY1 REPO domain necessary for YY1 PcG function, and used this mutant (YY1ΔREPO), to transduce bone marrow from YY1 conditional KO mice. While wild-type YY1 rescued B-cell development, YY1ΔREPO failed to rescue the B-cell lineage yielding reduced numbers of B lineage cells. Although the IgH rearrangement pattern was normal, there was a selective impact at the Igκ locus that showed a dramatic skewing of the expressed Igκ repertoire. We found that the REPO domain interacts with proteins from the condensin and cohesin complexes, and that YY1, EZH2 and condensin proteins co-localize at numerous sites across the Ig kappa locus. Knock-down of a condensin subunit protein or YY1 reduced rearrangement of Igκ Vκ genes suggesting a direct role for YY1-condensin complexes in Igκ locus structure and rearrangement. © 2013 European Molecular Biology Organization. | en_US |
dc.language | eng | en_US |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.html | en_US |
dc.relation.ispartof | EMBO Journal | en_US |
dc.subject | B-Cell Development | en_US |
dc.subject | Immunoglobulin | en_US |
dc.subject | Polycomb | en_US |
dc.subject | Rearrangement | en_US |
dc.subject | Yy1 | en_US |
dc.title | YY1 controls Igκ repertoire and B-cell development, and localizes with condensin on the Igκ locus | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wang, J: junwen@hkucc.hku.hk | en_US |
dc.identifier.authority | Wang, J=rp00280 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1038/emboj.2013.66 | en_US |
dc.identifier.pmid | 23531880 | - |
dc.identifier.scopus | eid_2-s2.0-84876419201 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-84876419201&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 32 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.spage | 1168 | en_US |
dc.identifier.epage | 1182 | en_US |
dc.identifier.isi | WOS:000317748400011 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Pan, X=15036577200 | en_US |
dc.identifier.scopusauthorid | Papasani, M=6507375487 | en_US |
dc.identifier.scopusauthorid | Hao, Y=55629671200 | en_US |
dc.identifier.scopusauthorid | Calamito, M=16201821700 | en_US |
dc.identifier.scopusauthorid | Wei, F=55186434600 | en_US |
dc.identifier.scopusauthorid | Quinn, WJ=9232819800 | en_US |
dc.identifier.scopusauthorid | Basu, A=36672497400 | en_US |
dc.identifier.scopusauthorid | Wang, J=8950599500 | en_US |
dc.identifier.scopusauthorid | Hodawadekar, S=15044548300 | en_US |
dc.identifier.scopusauthorid | Zaprazna, K=32868288900 | en_US |
dc.identifier.scopusauthorid | Liu, H=55631183200 | en_US |
dc.identifier.scopusauthorid | Shi, Y=55630650100 | en_US |
dc.identifier.scopusauthorid | Allman, D=7006980158 | en_US |
dc.identifier.scopusauthorid | Cancro, M=7004335899 | en_US |
dc.identifier.scopusauthorid | Atchison, ML=7006495633 | en_US |
dc.identifier.issnl | 0261-4189 | - |