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- Publisher Website: 10.1016/j.ultrasmedbio.2013.08.003
- Scopus: eid_2-s2.0-84887816807
- PMID: 24063956
- WOS: WOS:000327133400020
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Article: Membrane perforation and recovery dynamics in microbubble-mediated sonoporation
Title | Membrane perforation and recovery dynamics in microbubble-mediated sonoporation |
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Authors | |
Keywords | Membrane perforation Membrane recovery Real-time confocal imaging Sonoporation Spatiotemporal dynamics |
Issue Date | 2013 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ultrasmedbio |
Citation | Ultrasound in Medicine and Biology, 2013, v. 39 n. 12, p. 2393-2405 How to Cite? |
Abstract | Transient sonoporation can essentially be epitomized by two fundamental processes: acoustically induced membrane perforation and its subsequent resealing. To provide insight into these processes, this article presents a new series of direct evidence on the membrane-level dynamics during and after an episode of sonoporation. Our direct observations were obtained from anchored fetal fibroblasts whose membrane topography was imaged in situ using real-time confocal microscopy. To facilitate controlled sonoporation at the single-cell level, microbubbles that can passively adhere to the cell membrane were first introduced at a 1:1 cell-to-bubble ratio. Single-pulse ultrasound exposure (1-MHz frequency, 10-cycle pulse duration, 0.85-MPa peak negative pressure in situ) was then applied to trigger microbubble pulsation/collapse, which, in turn, instigated membrane perforation. With this protocol, five membrane-level phenomena were observed: (i) localized perforation of the cell membrane was synchronized with the instant of ultrasound pulsing; (ii) perforation sites with temporal peak area <30 μm(2) were resealed successfully; (iii) during recovery, a thickened pore rim emerged, and its temporal progression corresponded with the pore closure action; (iv) membrane resealing, if successful, would generally be completed within 1 min of the onset of sonoporation, and the resealing time constant was estimated to be below 20 s; (v) membrane resealing would fail for overly large pores (>100 μm(2)) or in the absence of extracellular calcium ions. These findings serve to underscore the spatiotemporal complexity of membrane-level dynamics in sonoporation. |
Persistent Identifier | http://hdl.handle.net/10722/189047 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.716 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Hu, Y | - |
dc.contributor.author | Wan, JMF | - |
dc.contributor.author | Yu, ACH | - |
dc.date.accessioned | 2013-09-17T14:24:47Z | - |
dc.date.available | 2013-09-17T14:24:47Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Ultrasound in Medicine and Biology, 2013, v. 39 n. 12, p. 2393-2405 | - |
dc.identifier.issn | 0301-5629 | - |
dc.identifier.uri | http://hdl.handle.net/10722/189047 | - |
dc.description.abstract | Transient sonoporation can essentially be epitomized by two fundamental processes: acoustically induced membrane perforation and its subsequent resealing. To provide insight into these processes, this article presents a new series of direct evidence on the membrane-level dynamics during and after an episode of sonoporation. Our direct observations were obtained from anchored fetal fibroblasts whose membrane topography was imaged in situ using real-time confocal microscopy. To facilitate controlled sonoporation at the single-cell level, microbubbles that can passively adhere to the cell membrane were first introduced at a 1:1 cell-to-bubble ratio. Single-pulse ultrasound exposure (1-MHz frequency, 10-cycle pulse duration, 0.85-MPa peak negative pressure in situ) was then applied to trigger microbubble pulsation/collapse, which, in turn, instigated membrane perforation. With this protocol, five membrane-level phenomena were observed: (i) localized perforation of the cell membrane was synchronized with the instant of ultrasound pulsing; (ii) perforation sites with temporal peak area <30 μm(2) were resealed successfully; (iii) during recovery, a thickened pore rim emerged, and its temporal progression corresponded with the pore closure action; (iv) membrane resealing, if successful, would generally be completed within 1 min of the onset of sonoporation, and the resealing time constant was estimated to be below 20 s; (v) membrane resealing would fail for overly large pores (>100 μm(2)) or in the absence of extracellular calcium ions. These findings serve to underscore the spatiotemporal complexity of membrane-level dynamics in sonoporation. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ultrasmedbio | - |
dc.relation.ispartof | Ultrasound in Medicine and Biology | - |
dc.subject | Membrane perforation | - |
dc.subject | Membrane recovery | - |
dc.subject | Real-time confocal imaging | - |
dc.subject | Sonoporation | - |
dc.subject | Spatiotemporal dynamics | - |
dc.title | Membrane perforation and recovery dynamics in microbubble-mediated sonoporation | - |
dc.type | Article | - |
dc.identifier.email | Wan, JMF: jmfwan@hku.hk | - |
dc.identifier.email | Yu, ACH: alfred.yu@hku.hk | - |
dc.identifier.authority | Wan, JMF=rp00798 | - |
dc.identifier.authority | Yu, ACH=rp00657 | - |
dc.identifier.doi | 10.1016/j.ultrasmedbio.2013.08.003 | - |
dc.identifier.pmid | 24063956 | - |
dc.identifier.scopus | eid_2-s2.0-84887816807 | - |
dc.identifier.hkuros | 222132 | - |
dc.identifier.volume | 39 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 2393 | - |
dc.identifier.epage | 2405 | - |
dc.identifier.isi | WOS:000327133400020 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0301-5629 | - |