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Conference Paper: Remineralization of Dentin in hydrogel microenvirment aided by Electrophoresis
Title | Remineralization of Dentin in hydrogel microenvirment aided by Electrophoresis |
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Authors | |
Keywords | Biomimetic mineralization Dental materials and Dentin |
Issue Date | 2013 |
Publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 |
Citation | The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 736 How to Cite? |
Abstract | Objective: Remineralization of dentin collagen matrix is a challenge work, and the velocity of remineralization is too slow for its clinic application. Herein, we developed a system to quickly remineralize dentin collagen matrix with calcium and phosphate loaded hydrogel microenvirment aiding with electrophoresis.
Method: The hydrogel mineralization system was constructed by 1% agarose gel containing 0.13 M CaCl2 ,1% pure gel, and 1% agarose gel containing 0.26 M Na2HPO4 in proper sequence, and the acid-etched dentin (37% H3PO4 for 2 min)was standing vertically in the junction of pure gel and Na2HPO4 loaded gel, which was connected to a electrophoresis apparatus (U=50V, I=20mA).electrophoresis for 2 hours was used as a mineralization cycle, and the all the gel systems were refreshed for the following mineralization cycles.
Result: After 6 cycles of mineralization, FTIR and XRD confirmed the mineral precipitates in the dentin slices were hydroxyapatite (HAp). SEM results showed that demineralized dentin collagen matrix were remineralized with intrafibrillar and interfibrillar HAp formation to duplicate the calcified dentin collagen matrix, the precipitates occluded the dentinal tubules, and the needle HA crystals densely packing with each other similar to enamel precipitated on remineralized dentin surface. Nanoindentation and microhardness results showed that the remineralization improved mechanical property of demineralized dentin.
Conclusion: A novel system of quick mineralization of dentin, which contained calcium and phosphate loaded agarose hydrogel microenvirment aiding with electrophoresis, was developed. The system may be a good candidate to treat dentin hypersensitivity, and other dentin defects. |
Description | Conference Theme: We are the Future Oral Presentation Session 29: O20 |
Persistent Identifier | http://hdl.handle.net/10722/192516 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.909 |
DC Field | Value | Language |
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dc.contributor.author | Wu, X | en_US |
dc.contributor.author | Xu, Q | en_US |
dc.contributor.author | Li, Q | en_US |
dc.contributor.author | Cao, Y | en_US |
dc.contributor.author | Chu, CH | en_US |
dc.date.accessioned | 2013-11-15T02:20:46Z | - |
dc.date.available | 2013-11-15T02:20:46Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 736 | en_US |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | http://hdl.handle.net/10722/192516 | - |
dc.description | Conference Theme: We are the Future | - |
dc.description | Oral Presentation | - |
dc.description | Session 29: O20 | - |
dc.description.abstract | Objective: Remineralization of dentin collagen matrix is a challenge work, and the velocity of remineralization is too slow for its clinic application. Herein, we developed a system to quickly remineralize dentin collagen matrix with calcium and phosphate loaded hydrogel microenvirment aiding with electrophoresis. Method: The hydrogel mineralization system was constructed by 1% agarose gel containing 0.13 M CaCl2 ,1% pure gel, and 1% agarose gel containing 0.26 M Na2HPO4 in proper sequence, and the acid-etched dentin (37% H3PO4 for 2 min)was standing vertically in the junction of pure gel and Na2HPO4 loaded gel, which was connected to a electrophoresis apparatus (U=50V, I=20mA).electrophoresis for 2 hours was used as a mineralization cycle, and the all the gel systems were refreshed for the following mineralization cycles. Result: After 6 cycles of mineralization, FTIR and XRD confirmed the mineral precipitates in the dentin slices were hydroxyapatite (HAp). SEM results showed that demineralized dentin collagen matrix were remineralized with intrafibrillar and interfibrillar HAp formation to duplicate the calcified dentin collagen matrix, the precipitates occluded the dentinal tubules, and the needle HA crystals densely packing with each other similar to enamel precipitated on remineralized dentin surface. Nanoindentation and microhardness results showed that the remineralization improved mechanical property of demineralized dentin. Conclusion: A novel system of quick mineralization of dentin, which contained calcium and phosphate loaded agarose hydrogel microenvirment aiding with electrophoresis, was developed. The system may be a good candidate to treat dentin hypersensitivity, and other dentin defects. | - |
dc.language | eng | en_US |
dc.publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 | - |
dc.relation.ispartof | Journal of Dental Research | en_US |
dc.rights | Journal of Dental Research. Copyright © Sage Publications, Inc. | - |
dc.subject | Biomimetic mineralization | - |
dc.subject | Dental materials and Dentin | - |
dc.title | Remineralization of Dentin in hydrogel microenvirment aided by Electrophoresis | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Li, Q: ql-li@126.com | en_US |
dc.identifier.email | Chu, CH: chchu@hku.hk | en_US |
dc.identifier.authority | Chu, CH=rp00022 | en_US |
dc.identifier.hkuros | 226771 | en_US |
dc.identifier.volume | 92 | en_US |
dc.identifier.issue | Special Issue B: abstract no. 736 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-0345 | - |