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Article: Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection

TitleComparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection
Authors
Issue Date2015
Citation
Eurosurveillance, 2015, v. 20 n. 41, pii=30042 How to Cite?
AbstractPlaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous.
Persistent Identifierhttp://hdl.handle.net/10722/222548
ISSN
2023 Impact Factor: 9.9
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ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPark, SW-
dc.contributor.authorPerera, RAPM-
dc.contributor.authorChoe, PG-
dc.contributor.authorLau, EHY-
dc.contributor.authorChoi, SJ-
dc.contributor.authorChun, JY-
dc.contributor.authorOh, HS-
dc.contributor.authorSong, K-
dc.contributor.authorBang, JH-
dc.contributor.authorKim, ES-
dc.contributor.authorKim, HB-
dc.contributor.authorPark, WB-
dc.contributor.authorKim, NJ-
dc.contributor.authorPoon, LLM-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorOh, MD-
dc.date.accessioned2016-01-18T07:42:26Z-
dc.date.available2016-01-18T07:42:26Z-
dc.date.issued2015-
dc.identifier.citationEurosurveillance, 2015, v. 20 n. 41, pii=30042-
dc.identifier.issn1025-496X-
dc.identifier.urihttp://hdl.handle.net/10722/222548-
dc.description.abstractPlaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous.-
dc.languageeng-
dc.relation.ispartofEuro Surveillance-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleComparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection-
dc.typeArticle-
dc.identifier.emailPerera, RAPM: mahenp@hku.hk-
dc.identifier.emailLau, EHY: ehylau@hku.hk-
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.authorityLau, EHY=rp01349-
dc.identifier.authorityPoon, LLM=rp00484-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.2807/1560-7917.ES.2015.20.41.30042-
dc.identifier.pmid26538277-
dc.identifier.scopuseid_2-s2.0-84965091767-
dc.identifier.hkuros256616-
dc.identifier.volume20-
dc.identifier.issue41-
dc.identifier.spagepii=30042-
dc.identifier.epagepii=30042-
dc.identifier.eissn1560-7917-
dc.identifier.isiWOS:000363410900003-
dc.identifier.issnl1025-496X-

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