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- Publisher Website: 10.1016/j.yexcr.2016.09.010
- Scopus: eid_2-s2.0-84994877140
- PMID: 27658568
- WOS: WOS:000386866100004
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Article: Overexpression of microRNA-1288 in oesophageal squamous cell carcinoma
Title | Overexpression of microRNA-1288 in oesophageal squamous cell carcinoma |
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Authors | |
Keywords | Squamous cell carcinoma FOXO1 miR-1288 Oesophageal |
Issue Date | 2016 |
Citation | Experimental Cell Research, 2016, v. 348, n. 2, p. 146-154 How to Cite? |
Abstract | © 2016 Elsevier Inc. Purpose This study aims to examine the expression profiles miR-1288 in oesophageal squamous cell carcinoma (ESCC). The cellular implications and target interactions of ESCC cells following miR-1288 overexpression was also examined. Methods In total, 120 oesophageal tissues (90 primary ESCCs and 30 non-neoplastic tissues) were recruited for miR-1288 expression analysis using qRT-PCR. An exogenous miR-1288 mimic and its inhibitor were used to explore the in-vitro effects of miR-1288 on ESCC cells by performing cell proliferation, colony formation, cell invasion and migration assays. Localisation and modulatory changes of various miR-1288 regulated proteins such as FOXO1, p53, TAB3, BCL2 and kRAS was examined using immunofluorescence and western blot. Results Overexpression of miR-1288 was more often noted in ESCC tissues when compared to non-neoplastic oesophageal tissues. High expression was often noted in high grade carcinomas and with metastases. Patients with high levels of miR-1288 expression showed a slightly better survival compared to patients with low miR-1288 levels. Furthermore, overexpression of miR-1288 showed increased cell proliferation and colony formation, improved cell migration and enhanced cell invasion properties in ESCC cells. In addition, miR-1288 overexpression in ESCC cells showed repression of cytoplasmic tumour suppressor FOXO1 protein expression. Inversely, inhibition of miR-1288 expression exhibited remarkable upregulation of FOXO1 protein, while expressions of other tested proteins remain unchanged. Conclusions Up regulation of miR-1288 expression in ESCC tissues and miR-1288 induced oncogenic features of ESCC cells in-vitro indicates the oncogenic roles of miR-1288 in ESCCs. Overexpression of miR-1288 play a key role in the pathogenesis of ESCCs and its modulation may have potential therapeutic value in patients with ESCC. |
Persistent Identifier | http://hdl.handle.net/10722/244060 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 0.947 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Gopalan, Vinod | - |
dc.contributor.author | Islam, Farhadul | - |
dc.contributor.author | Pillai, Suja | - |
dc.contributor.author | Tang, Johnny Cheuk On | - |
dc.contributor.author | Tong, Daniel King Hung | - |
dc.contributor.author | Law, Simon | - |
dc.contributor.author | Chan, Kwok Wah | - |
dc.contributor.author | Lam, Alfred King Yin | - |
dc.date.accessioned | 2017-08-31T08:55:56Z | - |
dc.date.available | 2017-08-31T08:55:56Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Experimental Cell Research, 2016, v. 348, n. 2, p. 146-154 | - |
dc.identifier.issn | 0014-4827 | - |
dc.identifier.uri | http://hdl.handle.net/10722/244060 | - |
dc.description.abstract | © 2016 Elsevier Inc. Purpose This study aims to examine the expression profiles miR-1288 in oesophageal squamous cell carcinoma (ESCC). The cellular implications and target interactions of ESCC cells following miR-1288 overexpression was also examined. Methods In total, 120 oesophageal tissues (90 primary ESCCs and 30 non-neoplastic tissues) were recruited for miR-1288 expression analysis using qRT-PCR. An exogenous miR-1288 mimic and its inhibitor were used to explore the in-vitro effects of miR-1288 on ESCC cells by performing cell proliferation, colony formation, cell invasion and migration assays. Localisation and modulatory changes of various miR-1288 regulated proteins such as FOXO1, p53, TAB3, BCL2 and kRAS was examined using immunofluorescence and western blot. Results Overexpression of miR-1288 was more often noted in ESCC tissues when compared to non-neoplastic oesophageal tissues. High expression was often noted in high grade carcinomas and with metastases. Patients with high levels of miR-1288 expression showed a slightly better survival compared to patients with low miR-1288 levels. Furthermore, overexpression of miR-1288 showed increased cell proliferation and colony formation, improved cell migration and enhanced cell invasion properties in ESCC cells. In addition, miR-1288 overexpression in ESCC cells showed repression of cytoplasmic tumour suppressor FOXO1 protein expression. Inversely, inhibition of miR-1288 expression exhibited remarkable upregulation of FOXO1 protein, while expressions of other tested proteins remain unchanged. Conclusions Up regulation of miR-1288 expression in ESCC tissues and miR-1288 induced oncogenic features of ESCC cells in-vitro indicates the oncogenic roles of miR-1288 in ESCCs. Overexpression of miR-1288 play a key role in the pathogenesis of ESCCs and its modulation may have potential therapeutic value in patients with ESCC. | - |
dc.language | eng | - |
dc.relation.ispartof | Experimental Cell Research | - |
dc.subject | Squamous cell carcinoma | - |
dc.subject | FOXO1 | - |
dc.subject | miR-1288 | - |
dc.subject | Oesophageal | - |
dc.title | Overexpression of microRNA-1288 in oesophageal squamous cell carcinoma | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.yexcr.2016.09.010 | - |
dc.identifier.pmid | 27658568 | - |
dc.identifier.scopus | eid_2-s2.0-84994877140 | - |
dc.identifier.hkuros | 272945 | - |
dc.identifier.volume | 348 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 146 | - |
dc.identifier.epage | 154 | - |
dc.identifier.eissn | 1090-2422 | - |
dc.identifier.isi | WOS:000386866100004 | - |
dc.identifier.issnl | 0014-4827 | - |