File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Effects of Riboflavin and Ultraviolet Illumination on the Biomechanical Properties of Conjunctiva

TitleEffects of Riboflavin and Ultraviolet Illumination on the Biomechanical Properties of Conjunctiva
Authors
KeywordsBiomechanical properties
Collagen crosslinking
Conjunctiva
Covalent bond
Riboflavin
Issue Date2018
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/ORE
Citation
Ophthalmic Research: journal for research in experimental and clinical ophthalmology, 2018, v. 60 n. 2, p. 87-93 How to Cite?
AbstractPurpose: Our experiment evaluated the biomechanical effects of riboflavin and ultraviolet A illumination, in terms of covalent bonding, permeability, and indentation rupture force, on excised cattle conjunctiva. Materials and Methods: Cattle conjunctiva was extracted and divided into the treatment group, which was soaked in riboflavin, followed by ultraviolet light illumination, and the control group. Samples were subsequently analysed on a Fourier transform infrared spectrum test to identify the presence of amide bonds, a permeability test to assess the diffusion of methylene blue across conjunctiva, and an indentation rupture force test. Results: The Fourier transform infrared spectrum test showed a significantly increased amide I band after treatment (p = 0.043), as well as in amide II, amide A, and amide B bands. The permeability test showed a reduced permeability to methylene blue after treatment (n = 25) compared to the control (n = 24; p = 0.03). The indentation rupture test showed a higher maximal rupture force in the treated samples (n = 10; p = 0.07). Conclusions: The study suggested that riboflavin and ultraviolet A illumination (commercially available as collagen crosslinking) strengthened cattle conjunctiva by increasing its amount of covalent bonds, reducing its permeability, and possibly improving its resistance to rupture. This is the first study in the collagen crosslinking literature on the biomechanical properties of conjunctival tissue. © 2017 S. Karger AG, Basel. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/248289
ISSN
2020 Impact Factor: 2.892
2020 SCImago Journal Rankings: 0.893
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, HMH-
dc.contributor.authorChoy, NKB-
dc.contributor.authorLai, JSM-
dc.date.accessioned2017-10-18T08:40:52Z-
dc.date.available2017-10-18T08:40:52Z-
dc.date.issued2018-
dc.identifier.citationOphthalmic Research: journal for research in experimental and clinical ophthalmology, 2018, v. 60 n. 2, p. 87-93-
dc.identifier.issn0030-3747-
dc.identifier.urihttp://hdl.handle.net/10722/248289-
dc.description.abstractPurpose: Our experiment evaluated the biomechanical effects of riboflavin and ultraviolet A illumination, in terms of covalent bonding, permeability, and indentation rupture force, on excised cattle conjunctiva. Materials and Methods: Cattle conjunctiva was extracted and divided into the treatment group, which was soaked in riboflavin, followed by ultraviolet light illumination, and the control group. Samples were subsequently analysed on a Fourier transform infrared spectrum test to identify the presence of amide bonds, a permeability test to assess the diffusion of methylene blue across conjunctiva, and an indentation rupture force test. Results: The Fourier transform infrared spectrum test showed a significantly increased amide I band after treatment (p = 0.043), as well as in amide II, amide A, and amide B bands. The permeability test showed a reduced permeability to methylene blue after treatment (n = 25) compared to the control (n = 24; p = 0.03). The indentation rupture test showed a higher maximal rupture force in the treated samples (n = 10; p = 0.07). Conclusions: The study suggested that riboflavin and ultraviolet A illumination (commercially available as collagen crosslinking) strengthened cattle conjunctiva by increasing its amount of covalent bonds, reducing its permeability, and possibly improving its resistance to rupture. This is the first study in the collagen crosslinking literature on the biomechanical properties of conjunctival tissue. © 2017 S. Karger AG, Basel. All rights reserved.-
dc.languageeng-
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/ORE-
dc.relation.ispartofOphthalmic Research: journal for research in experimental and clinical ophthalmology-
dc.rightsOphthalmic Research: journal for research in experimental and clinical ophthalmology. Copyright © S Karger AG.-
dc.rightsThis is the peer-reviewed but unedited manuscript version of the following article: [insert full citation, e.g., Cytogenet Genome Res 2014;142:227–238 (DOI: 10.1159/000361001)]. The final, published version is available at http://www.karger.com/?doi=[insert DOI number]. OR This is the un-reviewed and unedited manuscript version of the following article: [insert full citation, e.g., Cytogenet Genome Res 2014;142:227–238 (DOI: 10.1159/000361001)]. The final, published version is available at http://www.karger.com/?doi=[insert DOI number].-
dc.subjectBiomechanical properties-
dc.subjectCollagen crosslinking-
dc.subjectConjunctiva-
dc.subjectCovalent bond-
dc.subjectRiboflavin-
dc.titleEffects of Riboflavin and Ultraviolet Illumination on the Biomechanical Properties of Conjunctiva-
dc.typeArticle-
dc.identifier.emailChoy, NKB: bnkchoy@hku.hk-
dc.identifier.emailLai, JSM: laism@hku.hk-
dc.identifier.authorityChoy, NKB=rp01795-
dc.identifier.authorityLai, JSM=rp00295-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000478051-
dc.identifier.pmid28813714-
dc.identifier.scopuseid_2-s2.0-85027676209-
dc.identifier.hkuros279870-
dc.identifier.volume60-
dc.identifier.issue(2-
dc.identifier.spage87-
dc.identifier.epage93-
dc.identifier.isiWOS:000440839600004-
dc.publisher.placeSwitzerland-
dc.identifier.issnl0030-3747-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats