File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: RHCG suppresses tumorigenicity and metastasis in esophageal squamous cell carcinoma via inhibiting NF-κB signaling and MMP1 expression

TitleRHCG suppresses tumorigenicity and metastasis in esophageal squamous cell carcinoma via inhibiting NF-κB signaling and MMP1 expression
Authors
KeywordsESCC
Metastasis
MMP1
NF-κB
RHCG
Issue Date2018
PublisherIvyspring International Publisher. The Journal's web site is located at http://www.thno.org/
Citation
Theranostics, 2018, v. 8 n. 1, p. 185-198 How to Cite?
AbstractBackground and Aims: Esophageal squamous cell carcinoma (ESCC), a major histologic subtype of esophageal cancer, is increasing in incidence, but the genetic underpinnings of this disease remain unexplored. The aim of this study is to identify the recurrent genetic changes, elucidate their roles and discover new biomarkers for improving clinical management of ESCC. Methods: Western blotting and immunohistochemistry were performed to detect the expression level of RHCG. Bisulfite genomic sequencing (BGS) and methylation-specific PCR (MSP) were used to study the methylation status in the promoter region of RHCG. The tumor-suppressive effect of RHCG was determined by both in-vitro and in-vivo assays. Affymetrix cDNA microarray was used to identify the underlying molecular mechanism. Results:RHCG was frequently downregulated in ESCCs, which was significantly correlated with poor differentiation (P = 0.001), invasion (P = 0.003), lymph node metastasis (P = 0.038) and poorer prognosis (P < 0.001). Demethylation treatment and bisulfite genomic sequencing analyses revealed that the downregulation of RHCG in both ESCC cell lines and clinical samples was associated with its promoter hypermethylation. Functional assays demonstrated that RHCG could inhibit clonogenicity, cell motility, tumor formation and metastasis in mice. Further study revealed that RHCG could stabilize IκB by decreasing its phosphorylation, and subsequently inhibit NF-κB/p65 activation by blocking the nuclear translocation of p65, where it acted as a transcription regulator for the upregulation of MMP1 expression. Conclusions: Our results support the notion that RHCG is a novel tumor suppressor gene that plays an important role in the development and progression of ESCC.
Persistent Identifierhttp://hdl.handle.net/10722/256194
ISSN
2023 Impact Factor: 12.4
2023 SCImago Journal Rankings: 2.912
PubMed Central ID
ISI Accession Number ID
Grants

 

DC FieldValueLanguage
dc.contributor.authorMing, X-
dc.contributor.authorZhang, X-
dc.contributor.authorCao, T-
dc.contributor.authorZhang, L-
dc.contributor.authorQi, J-
dc.contributor.authorKam, NW-
dc.contributor.authorTang, X-
dc.contributor.authorCui, Y-
dc.contributor.authorZhang, B-
dc.contributor.authorLi, Y-
dc.contributor.authorQin, Y-
dc.contributor.authorGuan, X-
dc.date.accessioned2018-07-20T06:30:47Z-
dc.date.available2018-07-20T06:30:47Z-
dc.date.issued2018-
dc.identifier.citationTheranostics, 2018, v. 8 n. 1, p. 185-198-
dc.identifier.issn1838-7640-
dc.identifier.urihttp://hdl.handle.net/10722/256194-
dc.description.abstractBackground and Aims: Esophageal squamous cell carcinoma (ESCC), a major histologic subtype of esophageal cancer, is increasing in incidence, but the genetic underpinnings of this disease remain unexplored. The aim of this study is to identify the recurrent genetic changes, elucidate their roles and discover new biomarkers for improving clinical management of ESCC. Methods: Western blotting and immunohistochemistry were performed to detect the expression level of RHCG. Bisulfite genomic sequencing (BGS) and methylation-specific PCR (MSP) were used to study the methylation status in the promoter region of RHCG. The tumor-suppressive effect of RHCG was determined by both in-vitro and in-vivo assays. Affymetrix cDNA microarray was used to identify the underlying molecular mechanism. Results:RHCG was frequently downregulated in ESCCs, which was significantly correlated with poor differentiation (P = 0.001), invasion (P = 0.003), lymph node metastasis (P = 0.038) and poorer prognosis (P < 0.001). Demethylation treatment and bisulfite genomic sequencing analyses revealed that the downregulation of RHCG in both ESCC cell lines and clinical samples was associated with its promoter hypermethylation. Functional assays demonstrated that RHCG could inhibit clonogenicity, cell motility, tumor formation and metastasis in mice. Further study revealed that RHCG could stabilize IκB by decreasing its phosphorylation, and subsequently inhibit NF-κB/p65 activation by blocking the nuclear translocation of p65, where it acted as a transcription regulator for the upregulation of MMP1 expression. Conclusions: Our results support the notion that RHCG is a novel tumor suppressor gene that plays an important role in the development and progression of ESCC.-
dc.languageeng-
dc.publisherIvyspring International Publisher. The Journal's web site is located at http://www.thno.org/-
dc.relation.ispartofTheranostics-
dc.rightsTheranostics. Copyright © Ivyspring International Publisher.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectESCC-
dc.subjectMetastasis-
dc.subjectMMP1-
dc.subjectNF-κB-
dc.subjectRHCG-
dc.titleRHCG suppresses tumorigenicity and metastasis in esophageal squamous cell carcinoma via inhibiting NF-κB signaling and MMP1 expression-
dc.typeArticle-
dc.identifier.emailKam, NW: nwkam@hku.hk-
dc.identifier.emailGuan, X: xyguan@hku.hk-
dc.identifier.authorityGuan, X=rp00454-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.7150/thno.21383-
dc.identifier.pmcidPMC5743468-
dc.identifier.scopuseid_2-s2.0-85035087713-
dc.identifier.hkuros286046-
dc.identifier.hkuros290325-
dc.identifier.volume8-
dc.identifier.issue1-
dc.identifier.spage185-
dc.identifier.epage198-
dc.identifier.isiWOS:000417132000005-
dc.publisher.placeAustralia-
dc.relation.projectInvestigation of effects and molecular mechanisms of FGFR2-positive cancer-associate fibroblasts on tumor microenvironment in esophageal squamous cell carcinoma-
dc.identifier.issnl1838-7640-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats