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- Publisher Website: 10.1158/1538-7445.AM2019-696
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Conference Paper: High-throughput screening of therapeutic combinations against ovarian cancer
Title | High-throughput screening of therapeutic combinations against ovarian cancer |
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Authors | |
Issue Date | 2019 |
Publisher | American Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/ |
Citation | Proceedings of the American Association for Cancer Research (AACR) Annual Meeting 2019, Atlanta, USA, 29 March - 3 April 2019. In Cancer Research, 2019, v. 79 n. 13, Suppl., Abstract 696 How to Cite? |
Abstract | Applying synergistic drug combinations can increase efficacy of cancer treatment. However, conventional methods for identifying those combinations involve testing of every possible combination including different dosages for each agent, are difficult to scale due to the huge amount of efforts and costs needed. To tackle this problem, we have developed CombiGEM-CRISPR (combinatorial genetics en masse-CRISPR) to enable high-throughput assembly and screening of pair-wise gene combinations. By applying CombiGEM-CRISPR,phenotypes of combinatorial gene knockouts could be used to predict the effects of drug inhibitor combinations targeting the matching gene products. We designed a pair-wise sgRNA library comprising 25,281 combinations for interrogating 52 genes that are overexpressed in ovarian cancers. By pooled evaluation of their effects on cell proliferation, our screening identified new combinations that inhibit ovarian cancer cell growth. Therefore, our work accelerates the discovery of new therapeutic options against ovarian cancer through applying CombiGEM-CRISPR. |
Description | Session PO.BSB02.01 - Convergence Science for Therapeutics and Precision Medicine |
Persistent Identifier | http://hdl.handle.net/10722/274599 |
ISSN | 2023 Impact Factor: 12.5 2023 SCImago Journal Rankings: 3.468 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhou, P | - |
dc.contributor.author | Wong, SL | - |
dc.date.accessioned | 2019-08-18T15:05:02Z | - |
dc.date.available | 2019-08-18T15:05:02Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Proceedings of the American Association for Cancer Research (AACR) Annual Meeting 2019, Atlanta, USA, 29 March - 3 April 2019. In Cancer Research, 2019, v. 79 n. 13, Suppl., Abstract 696 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.uri | http://hdl.handle.net/10722/274599 | - |
dc.description | Session PO.BSB02.01 - Convergence Science for Therapeutics and Precision Medicine | - |
dc.description.abstract | Applying synergistic drug combinations can increase efficacy of cancer treatment. However, conventional methods for identifying those combinations involve testing of every possible combination including different dosages for each agent, are difficult to scale due to the huge amount of efforts and costs needed. To tackle this problem, we have developed CombiGEM-CRISPR (combinatorial genetics en masse-CRISPR) to enable high-throughput assembly and screening of pair-wise gene combinations. By applying CombiGEM-CRISPR,phenotypes of combinatorial gene knockouts could be used to predict the effects of drug inhibitor combinations targeting the matching gene products. We designed a pair-wise sgRNA library comprising 25,281 combinations for interrogating 52 genes that are overexpressed in ovarian cancers. By pooled evaluation of their effects on cell proliferation, our screening identified new combinations that inhibit ovarian cancer cell growth. Therefore, our work accelerates the discovery of new therapeutic options against ovarian cancer through applying CombiGEM-CRISPR. | - |
dc.language | eng | - |
dc.publisher | American Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/ | - |
dc.relation.ispartof | Cancer Research | - |
dc.relation.ispartof | Proceedings of the American Association for Cancer Research (AACR) Annual Meeting 2019 | - |
dc.title | High-throughput screening of therapeutic combinations against ovarian cancer | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Zhou, P: zhoupeng@hku.hk | - |
dc.identifier.email | Wong, SL: aslw@hku.hk | - |
dc.identifier.authority | Wong, SL=rp02139 | - |
dc.description.nature | abstract | - |
dc.identifier.doi | 10.1158/1538-7445.AM2019-696 | - |
dc.identifier.hkuros | 301306 | - |
dc.identifier.volume | 79 | - |
dc.identifier.issue | 13, Suppl. | - |
dc.identifier.spage | Abstract 696 | - |
dc.identifier.epage | Abstract 696 | - |
dc.identifier.isi | WOS:000488129902328 | - |
dc.publisher.place | United States | - |