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Conference Paper: Enhanced Osteogenic Differentiation by cAMP-loaded Layer-by-layer Self-assembly on Gelatin-Based Hydroxyapatite
Title | Enhanced Osteogenic Differentiation by cAMP-loaded Layer-by-layer Self-assembly on Gelatin-Based Hydroxyapatite |
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Authors | |
Issue Date | 2019 |
Publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ |
Citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, article no. 1553 How to Cite? |
Abstract | Objectives: To investigate osteogenic differentiation of cyclic adenosine monophosphate (cAMP) encapsulated stem cells from apical papilla (SCAPs) by layer-by-layer (LBL) self-assembly on gelatin based hydroxyapatite (HA) scaffold.
Methods: Gelatin gel were alternately soaked in solution of CaCl2 and Na2HPO4 to prepare the Gel-HA scaffold. The physico-chemical characteristics of Gel-HA were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). SCAPs were encapsulated with cAMP using LBL self-assembled gelatin and alginate polyelectrolytes (LBL-cAMP-SCAPs). SCAPs loaded LBL self-assembled without cAMP was used as negative control (LBL-SCAPs). The cellular morphology of LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA was analyzed by SEM for 21 days. Cell proliferation and viability was assessed by Cell Counting Kit-8 and live/dead staining, respectively after 1 and 7 days. The osteogenic differentiation of SCAPs was evaluated by alkaline phosphatase activity (ALP) and the expression of osteogenic related genes after 21 days.
Results: SEM revealed that interconnected pore architecture with HA crystals embedded within the gelatin surface. EDX confirmed the prominent element on the surface of Gel-HA was Calcium and Phosphorous. SEM revealed that both LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA exhibited spheroid morphology after 1 day, and filopodial structure after 7 days. LBL-cAMP-SCAPs formed extra cellular matrix on the surface of Gel-HA after 21 days. No significant difference in cell proliferation and viability was found between LBL-cAMP-SCAPs and LBL-SCAPs. LBL-cAMP-SCAPs seeded on the Gel-HA showed higher level of ALP activity than LBL-SCAPs.
Conclusions: cAMP encapsulated by LBL self-assembly enhanced osteogenic differentiation of SCAPs on gelatin based hydroxyapatite scaffold. |
Description | Poster Session: Dental Pulp Cells, Inflammatory and Regenerative Aspects - Presentation ID: 1553 |
Persistent Identifier | http://hdl.handle.net/10722/278683 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, JJ | - |
dc.contributor.author | Zhang, C | - |
dc.contributor.author | Chu, CH | - |
dc.date.accessioned | 2019-10-21T02:12:04Z | - |
dc.date.available | 2019-10-21T02:12:04Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, article no. 1553 | - |
dc.identifier.uri | http://hdl.handle.net/10722/278683 | - |
dc.description | Poster Session: Dental Pulp Cells, Inflammatory and Regenerative Aspects - Presentation ID: 1553 | - |
dc.description.abstract | Objectives: To investigate osteogenic differentiation of cyclic adenosine monophosphate (cAMP) encapsulated stem cells from apical papilla (SCAPs) by layer-by-layer (LBL) self-assembly on gelatin based hydroxyapatite (HA) scaffold. Methods: Gelatin gel were alternately soaked in solution of CaCl2 and Na2HPO4 to prepare the Gel-HA scaffold. The physico-chemical characteristics of Gel-HA were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). SCAPs were encapsulated with cAMP using LBL self-assembled gelatin and alginate polyelectrolytes (LBL-cAMP-SCAPs). SCAPs loaded LBL self-assembled without cAMP was used as negative control (LBL-SCAPs). The cellular morphology of LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA was analyzed by SEM for 21 days. Cell proliferation and viability was assessed by Cell Counting Kit-8 and live/dead staining, respectively after 1 and 7 days. The osteogenic differentiation of SCAPs was evaluated by alkaline phosphatase activity (ALP) and the expression of osteogenic related genes after 21 days. Results: SEM revealed that interconnected pore architecture with HA crystals embedded within the gelatin surface. EDX confirmed the prominent element on the surface of Gel-HA was Calcium and Phosphorous. SEM revealed that both LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA exhibited spheroid morphology after 1 day, and filopodial structure after 7 days. LBL-cAMP-SCAPs formed extra cellular matrix on the surface of Gel-HA after 21 days. No significant difference in cell proliferation and viability was found between LBL-cAMP-SCAPs and LBL-SCAPs. LBL-cAMP-SCAPs seeded on the Gel-HA showed higher level of ALP activity than LBL-SCAPs. Conclusions: cAMP encapsulated by LBL self-assembly enhanced osteogenic differentiation of SCAPs on gelatin based hydroxyapatite scaffold. | - |
dc.language | eng | - |
dc.publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ | - |
dc.relation.ispartof | Journal of Dental Research (Spec Issue) | - |
dc.relation.ispartof | IADR/AADR/CADR 2019 General Session & Exhibition | - |
dc.title | Enhanced Osteogenic Differentiation by cAMP-loaded Layer-by-layer Self-assembly on Gelatin-Based Hydroxyapatite | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.email | Chu, CH: chchu@hku.hk | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.identifier.authority | Chu, CH=rp00022 | - |
dc.identifier.hkuros | 307281 | - |
dc.identifier.hkuros | 329475 | - |
dc.identifier.volume | 98 | - |
dc.identifier.issue | Spec ISS A | - |
dc.identifier.spage | article no. 1553 | - |
dc.identifier.epage | article no. 1553 | - |
dc.publisher.place | United States | - |