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Article: Synthesis and Characterization of Fluoridated Silver Nanoparticles and Their Potential as a Non-Staining Anti-Caries Agent
Title | Synthesis and Characterization of Fluoridated Silver Nanoparticles and Their Potential as a Non-Staining Anti-Caries Agent |
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Authors | |
Keywords | silver nanoparticles fluoride dentin caries |
Issue Date | 2020 |
Publisher | Dove Medical Press Ltd. The Journal's web site is located at http://www.dovepress.com/articles.php?journal_id=5 |
Citation | International Journal of Nanomedicine, 2020, v. 15, p. 3207-3215 How to Cite? |
Abstract | Objectives: The first objective of this study was to prepare sodium fluoride (NaF) solution with various concentrations of polyethylene glycol-coated silver nanoparticles (PEG-AgNPs). The second objective was to study the antibacterial activity against Streptococcus mutans and the tooth-staining effect of the solution.
Methods: PEG-AgNPs were prepared via the one-step chemical reduction of silver acetate with thiolated polyethylene glycol. The PEG-AgNPs were characterized with ultraviolet-visible spectrometry and transmission electron microscopy. The half maximal inhibitory concentration (IC50) for the PEG-AgNPs against Streptococcus mutans and human gingival fibroblasts (HGF-1) were determined. The staining effect on dentin and enamel for the 2.5% NaF solutions with PEG-AgNPs at 12,800, 6400, 1600, and 400 ppm was investigated using digital spectrophotometry. The IC50 of the fluoridated silver nanoparticles against Streptococcus mutans were measured.
Results: The PEG-AgNPs have an average diameter of 2.56± 0.43 nm and showed excellent stability at high ionic strength (2.5% NaF) for 18 months. The IC50 of PEG-AgNPs against Streptococcus mutans was found to be 21.16± 1.08 ppm silver, which was half of IC50 against HGF-1 cells (42.36± 1.12 ppm), providing a working range to kill bacteria with no harm to human cells. The formulations with different concentrations of PEG-AgNPs showed no significant staining of teeth. Combining PEG-AgNPs with NaF significantly expanded the therapeutic window against Streptococcus mutans by reducing its IC50.
Conclusion: A biocompatible solution of NaF with PEG-AgNPs was developed. Because it has antibacterial activity against Streptococcus mutans and no tooth-staining effect, it can be used as an anti-caries agent. |
Persistent Identifier | http://hdl.handle.net/10722/286145 |
ISSN | 2010 Impact Factor: 4.976 2020 SCImago Journal Rankings: 1.245 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Yin, IX | - |
dc.contributor.author | Zhao, IS | - |
dc.contributor.author | Mei, ML | - |
dc.contributor.author | Lo, ECM | - |
dc.contributor.author | Tang, J | - |
dc.contributor.author | Li, Q | - |
dc.contributor.author | So, LY | - |
dc.contributor.author | Chu, CH | - |
dc.date.accessioned | 2020-08-31T06:59:46Z | - |
dc.date.available | 2020-08-31T06:59:46Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | International Journal of Nanomedicine, 2020, v. 15, p. 3207-3215 | - |
dc.identifier.issn | 1176-9114 | - |
dc.identifier.uri | http://hdl.handle.net/10722/286145 | - |
dc.description.abstract | Objectives: The first objective of this study was to prepare sodium fluoride (NaF) solution with various concentrations of polyethylene glycol-coated silver nanoparticles (PEG-AgNPs). The second objective was to study the antibacterial activity against Streptococcus mutans and the tooth-staining effect of the solution. Methods: PEG-AgNPs were prepared via the one-step chemical reduction of silver acetate with thiolated polyethylene glycol. The PEG-AgNPs were characterized with ultraviolet-visible spectrometry and transmission electron microscopy. The half maximal inhibitory concentration (IC50) for the PEG-AgNPs against Streptococcus mutans and human gingival fibroblasts (HGF-1) were determined. The staining effect on dentin and enamel for the 2.5% NaF solutions with PEG-AgNPs at 12,800, 6400, 1600, and 400 ppm was investigated using digital spectrophotometry. The IC50 of the fluoridated silver nanoparticles against Streptococcus mutans were measured. Results: The PEG-AgNPs have an average diameter of 2.56± 0.43 nm and showed excellent stability at high ionic strength (2.5% NaF) for 18 months. The IC50 of PEG-AgNPs against Streptococcus mutans was found to be 21.16± 1.08 ppm silver, which was half of IC50 against HGF-1 cells (42.36± 1.12 ppm), providing a working range to kill bacteria with no harm to human cells. The formulations with different concentrations of PEG-AgNPs showed no significant staining of teeth. Combining PEG-AgNPs with NaF significantly expanded the therapeutic window against Streptococcus mutans by reducing its IC50. Conclusion: A biocompatible solution of NaF with PEG-AgNPs was developed. Because it has antibacterial activity against Streptococcus mutans and no tooth-staining effect, it can be used as an anti-caries agent. | - |
dc.language | eng | - |
dc.publisher | Dove Medical Press Ltd. The Journal's web site is located at http://www.dovepress.com/articles.php?journal_id=5 | - |
dc.relation.ispartof | International Journal of Nanomedicine | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | silver | - |
dc.subject | nanoparticles | - |
dc.subject | fluoride | - |
dc.subject | dentin | - |
dc.subject | caries | - |
dc.title | Synthesis and Characterization of Fluoridated Silver Nanoparticles and Their Potential as a Non-Staining Anti-Caries Agent | - |
dc.type | Article | - |
dc.identifier.email | Lo, ECM: edward-lo@hku.hk | - |
dc.identifier.email | Tang, J: jinyao@hku.hk | - |
dc.identifier.email | Chu, CH: chchu@hku.hk | - |
dc.identifier.authority | Lo, ECM=rp00015 | - |
dc.identifier.authority | Tang, J=rp01677 | - |
dc.identifier.authority | Chu, CH=rp00022 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.2147/IJN.S243202 | - |
dc.identifier.pmid | 32440119 | - |
dc.identifier.pmcid | PMC7212993 | - |
dc.identifier.scopus | eid_2-s2.0-85085049833 | - |
dc.identifier.hkuros | 313558 | - |
dc.identifier.volume | 15 | - |
dc.identifier.spage | 3207 | - |
dc.identifier.epage | 3215 | - |
dc.identifier.isi | WOS:000530160700001 | - |
dc.publisher.place | New Zealand | - |
dc.identifier.issnl | 1176-9114 | - |