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Article: Surface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS

TitleSurface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS
Authors
KeywordsAntigen detections
Cervical cancers
Early diagnosis
HeLa cell lysate
Marker proteins
Issue Date2020
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/analyst
Citation
The Analyst, 2020, v. 145 n. 19, p. 6237-6242 How to Cite?
AbstractHPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.
Persistent Identifierhttp://hdl.handle.net/10722/289650
ISSN
2023 Impact Factor: 3.6
2023 SCImago Journal Rankings: 0.693
ISI Accession Number ID
Grants

 

DC FieldValueLanguage
dc.contributor.authorTAM, TSC-
dc.contributor.authorCHENG, YH-
dc.contributor.authorLok, CN-
dc.contributor.authorAu Yeung, HY-
dc.contributor.authorNi, WX-
dc.contributor.authorWei, XL-
dc.contributor.authorNg, KM-
dc.date.accessioned2020-10-22T08:15:32Z-
dc.date.available2020-10-22T08:15:32Z-
dc.date.issued2020-
dc.identifier.citationThe Analyst, 2020, v. 145 n. 19, p. 6237-6242-
dc.identifier.issn0003-2654-
dc.identifier.urihttp://hdl.handle.net/10722/289650-
dc.description.abstractHPV-induced cervical cancer is one of the most lethal cancers. Therefore, the development of a reliable and accurate method for the early diagnosis of HPV infections is highly important. Here, gold nanoparticles (AuNPs) were utilized as mass tags in an immuno-capture LI-MS assay for the detection of HPV marker proteins. Through the optimization of the amount of antibodies and surface charges on AuNPs, high antigen detection efficiency with minimal non-specific binding was achieved. With optimized antibody-conjugated AuNPs, low attomole amount of HPV proteins in HeLa cell lysate was quantified.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/analyst-
dc.relation.ispartofThe Analyst-
dc.subjectAntigen detections-
dc.subjectCervical cancers-
dc.subjectEarly diagnosis-
dc.subjectHeLa cell lysate-
dc.subjectMarker proteins-
dc.titleSurface optimization of gold nanoparticle mass tags for the sensitive detection of protein biomarkers via immuno-capture LI-MS-
dc.typeArticle-
dc.identifier.emailLok, CN: cnlok@hkucc.hku.hk-
dc.identifier.emailAu Yeung, HY: hoyuay@hku.hk-
dc.identifier.emailNg, KM: kwanmng@hku.hk-
dc.identifier.authorityLok, CN=rp00752-
dc.identifier.authorityAu Yeung, HY=rp01819-
dc.identifier.authorityNg, KM=rp00766-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/D0AN01121E-
dc.identifier.pmid32839801-
dc.identifier.scopuseid_2-s2.0-85091808523-
dc.identifier.hkuros315955-
dc.identifier.volume145-
dc.identifier.issue19-
dc.identifier.spage6237-
dc.identifier.epage6242-
dc.identifier.isiWOS:000573175200024-
dc.publisher.placeUnited Kingdom-
dc.relation.projectGold@Metal Sulfide (Noble Metal@Semiconductor) Nanoparticles: Enhancement of Laser Desorption/Ionization Efficiency via Plasmon-Exciton Coupling and Its Analytical Application in Mass Spectrometry-
dc.identifier.issnl0003-2654-

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