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Conference Paper: Timeless Interacts with PARP1 to Promote DNA DSB Repair
| Title | Timeless Interacts with PARP1 to Promote DNA DSB Repair |
|---|---|
| Authors | |
| Issue Date | 2019 |
| Citation | The 42nd Annual Meeting of the Molecular Biology Society of Japan, Fukuoka, Japan, 3-6 : December 2019 How to Cite? |
| Abstract | PARP1 is a major poly(ADP-ribose) transferase that plays an important role in DNA damage repair. PARP1 has been shown to be a DNA single-strand and double-strand break sensor protein that helps rapidly recruit many downstream DNA repair proteins to damaged DNA sites in a poly(ADP-ribose) dependent manner. In this presentation I will talk about a new mechanism of PARP1-mediated DNA damage response. We identified and provided the biochemical and structural evidence that PARP1 interacts with a critical protein called Timeless. We demonstrated that rapid and transient accumulation of Timeless at laser-induced DNA damage sites requires PARP1 but not poly(ADP-ribosyl)ation and that Timeless is co-trapped with PARP1 at DNA lesions upon the treatment of PARP1 inhibitor. Furthermore, we show that Timeless and PARP1 interaction is required for efficient DSB repair. |
| Persistent Identifier | http://hdl.handle.net/10722/298995 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Qian, C | - |
| dc.date.accessioned | 2021-04-22T04:50:32Z | - |
| dc.date.available | 2021-04-22T04:50:32Z | - |
| dc.date.issued | 2019 | - |
| dc.identifier.citation | The 42nd Annual Meeting of the Molecular Biology Society of Japan, Fukuoka, Japan, 3-6 : December 2019 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/298995 | - |
| dc.description.abstract | PARP1 is a major poly(ADP-ribose) transferase that plays an important role in DNA damage repair. PARP1 has been shown to be a DNA single-strand and double-strand break sensor protein that helps rapidly recruit many downstream DNA repair proteins to damaged DNA sites in a poly(ADP-ribose) dependent manner. In this presentation I will talk about a new mechanism of PARP1-mediated DNA damage response. We identified and provided the biochemical and structural evidence that PARP1 interacts with a critical protein called Timeless. We demonstrated that rapid and transient accumulation of Timeless at laser-induced DNA damage sites requires PARP1 but not poly(ADP-ribosyl)ation and that Timeless is co-trapped with PARP1 at DNA lesions upon the treatment of PARP1 inhibitor. Furthermore, we show that Timeless and PARP1 interaction is required for efficient DSB repair. | - |
| dc.language | eng | - |
| dc.relation.ispartof | The 42nd Annual Meeting of the Molecular Biology Society of Japan | - |
| dc.title | Timeless Interacts with PARP1 to Promote DNA DSB Repair | - |
| dc.type | Conference_Paper | - |
| dc.identifier.email | Qian, C: cmqian@hku.hk | - |
| dc.identifier.authority | Qian, C=rp01371 | - |
| dc.identifier.hkuros | 317297 | - |