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Conference Paper: Elucidating the role of PHD-finger protein PHF5A in neural crest specification

TitleElucidating the role of PHD-finger protein PHF5A in neural crest specification
Authors
Issue Date2021
Citation
The 2021 Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences, Virtual Meeting. Hong Kong, 12 June 2021 How to Cite?
AbstractIn vertebrates, the neural crest (NC) belongs to a migratory population of multipotent stem cells which arise at the interface between the neural and non-neural ectoderm. Our previous studies showed that a RhoGAP Deleted in Liver Cancer 1 (DLC1) plays an important role in governing NC specification through regulating the expression of NC specifier genes (SOX9, FOXD3 and SNAIL2) but the underlying mechanisms remain unknown. Here, by mass spectrometry, we identified a DLC1 interacting factor, PHD Finger Protein 5A (PHF5A) which has been implicated in regulating gene transcription[1] and splicing[2]. By in ovo electroporation, we found that CRISPR/Cas9-targeted PHF5A downregulated SOX9 expression while neural marker SOX2 was upregulated, indicating a cell fate switch from NC to neural. In addition to the in vivo interaction between DLC1-PHF5A, we further showed the association of DLC1 and PHF5A with a splicing factor 3B subunit 1 (SF3B1), suggesting a splicing complex to regulate gene expression at the post-transcriptional level[2]. Indeed, both DLC1 knockdown and PHF5A knockout resulted in intron retention of SOX9 and SNAIL2. Altogether, our findings reveal that a non-transcription factor DLC1 recruits PHF5A-SF3B1 complex to regulate pre-mRNA splicing of NC specifier genes, unravelling new molecular mechanism of NCC fate specification.
DescriptionPoster Presentation II- section 6
Jointly organized by the University of Hong Kong (HKU), the Chinese University of Hong Kong (CUHK) and the Hong Kong University of Science and Technology (HKUST).
Persistent Identifierhttp://hdl.handle.net/10722/300582

 

DC FieldValueLanguage
dc.contributor.authorZheng, Z-
dc.contributor.authorRao, Y-
dc.contributor.authorHui, MN-
dc.contributor.authorCheung, MPL-
dc.contributor.authorWong, KWK-
dc.contributor.authorSharma, R-
dc.contributor.authorCheung, MCH-
dc.date.accessioned2021-06-18T14:54:04Z-
dc.date.available2021-06-18T14:54:04Z-
dc.date.issued2021-
dc.identifier.citationThe 2021 Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences, Virtual Meeting. Hong Kong, 12 June 2021-
dc.identifier.urihttp://hdl.handle.net/10722/300582-
dc.descriptionPoster Presentation II- section 6-
dc.descriptionJointly organized by the University of Hong Kong (HKU), the Chinese University of Hong Kong (CUHK) and the Hong Kong University of Science and Technology (HKUST).-
dc.description.abstractIn vertebrates, the neural crest (NC) belongs to a migratory population of multipotent stem cells which arise at the interface between the neural and non-neural ectoderm. Our previous studies showed that a RhoGAP Deleted in Liver Cancer 1 (DLC1) plays an important role in governing NC specification through regulating the expression of NC specifier genes (SOX9, FOXD3 and SNAIL2) but the underlying mechanisms remain unknown. Here, by mass spectrometry, we identified a DLC1 interacting factor, PHD Finger Protein 5A (PHF5A) which has been implicated in regulating gene transcription[1] and splicing[2]. By in ovo electroporation, we found that CRISPR/Cas9-targeted PHF5A downregulated SOX9 expression while neural marker SOX2 was upregulated, indicating a cell fate switch from NC to neural. In addition to the in vivo interaction between DLC1-PHF5A, we further showed the association of DLC1 and PHF5A with a splicing factor 3B subunit 1 (SF3B1), suggesting a splicing complex to regulate gene expression at the post-transcriptional level[2]. Indeed, both DLC1 knockdown and PHF5A knockout resulted in intron retention of SOX9 and SNAIL2. Altogether, our findings reveal that a non-transcription factor DLC1 recruits PHF5A-SF3B1 complex to regulate pre-mRNA splicing of NC specifier genes, unravelling new molecular mechanism of NCC fate specification.-
dc.languageeng-
dc.relation.ispartof2021 Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences-
dc.titleElucidating the role of PHD-finger protein PHF5A in neural crest specification-
dc.typeConference_Paper-
dc.identifier.emailHui, MN: mnhui@hku.hk-
dc.identifier.emailCheung, MPL: mplcheun@hku.hk-
dc.identifier.emailWong, KWK: kwkw1017@hku.hk-
dc.identifier.emailSharma, R: rasharma@hku.hk-
dc.identifier.emailCheung, MCH: mcheung9@hku.hk-
dc.identifier.authorityCheung, MCH=rp00245-
dc.identifier.hkuros323010-

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