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Article: A novel dual-action antimicrobial peptide for caries management

TitleA novel dual-action antimicrobial peptide for caries management
Authors
KeywordsCaries
Prevention
Mineralisation
Peptides
Antimicrobial
Issue Date2021
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal of Dentistry, 2021, v. 111, article no. 103729 How to Cite?
AbstractObjectives: To develop a novel dual-action peptide with antimicrobial and mineralising properties. Methods: A novel peptide, namely GA-KR12, was synthesised through grafting gallic acid to KR12. The secondary structure of GA-KR12 was evaluated by circular dichroism spectroscopy. The stability was evaluated by high-performance liquid chromatography. The cytotoxicity was evaluated by a mitochondrial dehydrogenase activity assay. The antimicrobial properties against common cariogenic species were evaluated by minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). The morphology of cariogenic species was analysed by transmission electron microscope (TEM). To assess the mineralising effect of GA-KR12 on enamel, the lesion depths, mineral loss, surface morphology, calcium-to-phosphorus ratio and crystal characteristics were determined using micro-computed tomography, scanning electron microscopy (SEM) and energy dispersive spectroscopy X-ray diffraction, respectively. Results: GA-KR12 did not exhibit cytotoxicity against HGF. Around 82% of the GA-KR12 remained in human saliva at 37°C for 1 h. The MIC and MBC/MFC against the tested species were 10-320 μM and 20-1,280 μM, respectively. GA-KR12 induced remarkable morphological defects in the tested species. The enamel treated with GA-KR12 had smaller lesion depths (p < 0.001), less mineral loss (p < 0.001) and higher calcium-to-phosphorus molar ratios (p < 0.001) than those in the enamel treated with water. SEM showed a well-organised prism pattern in enamel treated with GA-KR12. X-ray diffraction revealed that the hydroxyapatite on the enamel treated with GA-KR12 was better crystalised. Conclusions: This study developed a biocompatible and stable peptide which inhibited the growth of cariogenic species and mineralised the enamel caries. Clinical significance The novel dual-action peptide, GA-KR12, is potential applicable in the management of caries.
Persistent Identifierhttp://hdl.handle.net/10722/300860
ISSN
2023 Impact Factor: 4.8
2023 SCImago Journal Rankings: 1.313
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNiu, JY-
dc.contributor.authorYin, IX-
dc.contributor.authorWu, WKK-
dc.contributor.authorLi, QL-
dc.contributor.authorMei, ML-
dc.contributor.authorChu, CH-
dc.date.accessioned2021-07-06T03:11:13Z-
dc.date.available2021-07-06T03:11:13Z-
dc.date.issued2021-
dc.identifier.citationJournal of Dentistry, 2021, v. 111, article no. 103729-
dc.identifier.issn0300-5712-
dc.identifier.urihttp://hdl.handle.net/10722/300860-
dc.description.abstractObjectives: To develop a novel dual-action peptide with antimicrobial and mineralising properties. Methods: A novel peptide, namely GA-KR12, was synthesised through grafting gallic acid to KR12. The secondary structure of GA-KR12 was evaluated by circular dichroism spectroscopy. The stability was evaluated by high-performance liquid chromatography. The cytotoxicity was evaluated by a mitochondrial dehydrogenase activity assay. The antimicrobial properties against common cariogenic species were evaluated by minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). The morphology of cariogenic species was analysed by transmission electron microscope (TEM). To assess the mineralising effect of GA-KR12 on enamel, the lesion depths, mineral loss, surface morphology, calcium-to-phosphorus ratio and crystal characteristics were determined using micro-computed tomography, scanning electron microscopy (SEM) and energy dispersive spectroscopy X-ray diffraction, respectively. Results: GA-KR12 did not exhibit cytotoxicity against HGF. Around 82% of the GA-KR12 remained in human saliva at 37°C for 1 h. The MIC and MBC/MFC against the tested species were 10-320 μM and 20-1,280 μM, respectively. GA-KR12 induced remarkable morphological defects in the tested species. The enamel treated with GA-KR12 had smaller lesion depths (p < 0.001), less mineral loss (p < 0.001) and higher calcium-to-phosphorus molar ratios (p < 0.001) than those in the enamel treated with water. SEM showed a well-organised prism pattern in enamel treated with GA-KR12. X-ray diffraction revealed that the hydroxyapatite on the enamel treated with GA-KR12 was better crystalised. Conclusions: This study developed a biocompatible and stable peptide which inhibited the growth of cariogenic species and mineralised the enamel caries. Clinical significance The novel dual-action peptide, GA-KR12, is potential applicable in the management of caries.-
dc.languageeng-
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent-
dc.relation.ispartofJournal of Dentistry-
dc.subjectCaries-
dc.subjectPrevention-
dc.subjectMineralisation-
dc.subjectPeptides-
dc.subjectAntimicrobial-
dc.titleA novel dual-action antimicrobial peptide for caries management-
dc.typeArticle-
dc.identifier.emailYin, IX: irisxyin@hku.hk-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.authorityChu, CH=rp00022-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jdent.2021.103729-
dc.identifier.pmid34146653-
dc.identifier.scopuseid_2-s2.0-85108601573-
dc.identifier.hkuros323114-
dc.identifier.volume111-
dc.identifier.spagearticle no. 103729-
dc.identifier.epagearticle no. 103729-
dc.identifier.isiWOS:000684505000001-
dc.publisher.placeUnited Kingdom-

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