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- Publisher Website: 10.1016/j.jdent.2021.103736
- Scopus: eid_2-s2.0-85114386498
- PMID: 34175452
- WOS: WOS:000684936100008
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Article: Remineralising dentine caries using an artificial antimicrobial peptide: An in vitro study
Title | Remineralising dentine caries using an artificial antimicrobial peptide: An in vitro study |
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Authors | |
Keywords | Caries Prevention Remineralization Demineralization Peptides |
Issue Date | 2021 |
Publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent |
Citation | Journal of Dentistry, 2021, v. 111, p. article no. 103736 How to Cite? |
Abstract | Objective To investigate the antibacterial and remineralising effects of a novel dual-action antimicrobial peptide, GA-KR12, on artificial dentine caries. Methods Human dentine blocks with artificial carious lesions were allocated to two groups – Group 1: dentine blocks treated with the novel antimicrobial peptide GA-KR12 twice daily; Group 2: dentine blocks received water as the negative control. Two groups underwent Streptococcus mutan biofilm–remineralisation cycles at 37 °C for 7 days. The morphology, viability and growth kinetics of the S. mutans biofilm were evaluated by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and colony-forming unit (CFU) counting, respectively. The dentine blocks’ lesion depths and mineral loss, changes in chemical structure, dentine surface morphology and crystal characteristics were determined using micro-computed tomography, Fourier transform infrared (FTIR), SEM and X-ray diffraction (XRD), respectively. Results The surface of the dentine blocks in Group 1 was partially covered by S. mutans with damaged cell structure. Group 2 showed affluent growth of S. mutans covering the dentine surface when compared to Group 1. The dead-to-live ratio of Group 1 and Group 2 were 0.78 ± 0.01 and 0.47 ± 0.08, respectively (p < 0.001). The Log CFUs of Group 1 and Group 2 were 7.14 ± 0.30 and 8.24 ± 0.20, respectively (p < 0.001). The lesion depths of Group 1 and Group 2 were 109 ± 1µm and 135 ± 3 µm, respectively (p < 0.001). The mineral loss of Group 1 and Group 2 were 0.59 ± 0.08 gHApcm-3 and 0.81 ± 0.07 gHApcm-3, respectively (p < 0.001). FTIR showed the amide I-to-hydrogen phosphate (HPO42−) ratios of Group 1 and Group 2 were 0.25 ± 0.05 and 0.39 ± 0.05 (p < 0.001), respectively. SEM images showed Group 1 had less exposed dentine collagen fibres than Group 2. The XRD revealed that the hydroxyapatite in Group 1 was well crystalised. Conclusion This study demonstrated that the novel antimicrobial peptide GA-KR12 inhibited the growth of S. mutans biofilm and enhanced the remineralisation of artificial dentine caries. |
Persistent Identifier | http://hdl.handle.net/10722/301918 |
ISSN | 2023 Impact Factor: 4.8 2023 SCImago Journal Rankings: 1.313 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Niu, JY | - |
dc.contributor.author | Yin, IX | - |
dc.contributor.author | Wu, WKK | - |
dc.contributor.author | Li, QL | - |
dc.contributor.author | Mei, ML | - |
dc.contributor.author | Chu, CH | - |
dc.date.accessioned | 2021-08-21T03:28:54Z | - |
dc.date.available | 2021-08-21T03:28:54Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Journal of Dentistry, 2021, v. 111, p. article no. 103736 | - |
dc.identifier.issn | 0300-5712 | - |
dc.identifier.uri | http://hdl.handle.net/10722/301918 | - |
dc.description.abstract | Objective To investigate the antibacterial and remineralising effects of a novel dual-action antimicrobial peptide, GA-KR12, on artificial dentine caries. Methods Human dentine blocks with artificial carious lesions were allocated to two groups – Group 1: dentine blocks treated with the novel antimicrobial peptide GA-KR12 twice daily; Group 2: dentine blocks received water as the negative control. Two groups underwent Streptococcus mutan biofilm–remineralisation cycles at 37 °C for 7 days. The morphology, viability and growth kinetics of the S. mutans biofilm were evaluated by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and colony-forming unit (CFU) counting, respectively. The dentine blocks’ lesion depths and mineral loss, changes in chemical structure, dentine surface morphology and crystal characteristics were determined using micro-computed tomography, Fourier transform infrared (FTIR), SEM and X-ray diffraction (XRD), respectively. Results The surface of the dentine blocks in Group 1 was partially covered by S. mutans with damaged cell structure. Group 2 showed affluent growth of S. mutans covering the dentine surface when compared to Group 1. The dead-to-live ratio of Group 1 and Group 2 were 0.78 ± 0.01 and 0.47 ± 0.08, respectively (p < 0.001). The Log CFUs of Group 1 and Group 2 were 7.14 ± 0.30 and 8.24 ± 0.20, respectively (p < 0.001). The lesion depths of Group 1 and Group 2 were 109 ± 1µm and 135 ± 3 µm, respectively (p < 0.001). The mineral loss of Group 1 and Group 2 were 0.59 ± 0.08 gHApcm-3 and 0.81 ± 0.07 gHApcm-3, respectively (p < 0.001). FTIR showed the amide I-to-hydrogen phosphate (HPO42−) ratios of Group 1 and Group 2 were 0.25 ± 0.05 and 0.39 ± 0.05 (p < 0.001), respectively. SEM images showed Group 1 had less exposed dentine collagen fibres than Group 2. The XRD revealed that the hydroxyapatite in Group 1 was well crystalised. Conclusion This study demonstrated that the novel antimicrobial peptide GA-KR12 inhibited the growth of S. mutans biofilm and enhanced the remineralisation of artificial dentine caries. | - |
dc.language | eng | - |
dc.publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent | - |
dc.relation.ispartof | Journal of Dentistry | - |
dc.subject | Caries | - |
dc.subject | Prevention | - |
dc.subject | Remineralization | - |
dc.subject | Demineralization | - |
dc.subject | Peptides | - |
dc.title | Remineralising dentine caries using an artificial antimicrobial peptide: An in vitro study | - |
dc.type | Article | - |
dc.identifier.email | Yin, IX: irisxyin@hku.hk | - |
dc.identifier.email | Chu, CH: chchu@hku.hk | - |
dc.identifier.authority | Chu, CH=rp00022 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jdent.2021.103736 | - |
dc.identifier.pmid | 34175452 | - |
dc.identifier.scopus | eid_2-s2.0-85114386498 | - |
dc.identifier.hkuros | 324185 | - |
dc.identifier.volume | 111 | - |
dc.identifier.spage | article no. 103736 | - |
dc.identifier.epage | article no. 103736 | - |
dc.identifier.isi | WOS:000684936100008 | - |
dc.publisher.place | United Kingdom | - |