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Conference Paper: The Tim3-galectin-9 interactions in the tumor microenvironment of nasopharyngeal cancer.
Title | The Tim3-galectin-9 interactions in the tumor microenvironment of nasopharyngeal cancer. |
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Authors | |
Issue Date | 2021 |
Publisher | American Society of Clinical Oncology. The Journal's web site is located at http://www.jco.org/ |
Citation | 2021 American Society of Clinical Oncology (ASCO) Annual Meeting, online meeting, 4-8 June 2021. In Journal of Clinical Oncology, 2021, v. 39 n. 15, Suppl., abstract no. 2629 How to Cite? |
Abstract | Background: The complex cell interactions within the tumor microenvironment (TME) have become a crucial point in cancer research. Yet, the cell interactions might not only depend on the frequency of immune cells, but also on the inter-individual distances as cells might interact via soluble factors and/or cell-cell contact. Accordingly, the mapping of TME has recently gained importance. The aim of this study is to investigate the alternations between galectin-9 (G9) and its natural immunosuppressive receptor, T cell immunoglobulin and mucin domain 3 (Tim3) in nasopharyngeal cancer (NPC). Methods: Using multiplexed quantitative immunofluorescence, we measured the levels of G9 and Tim3 in 95 NPC patients cancerous and 8 normal specimens in tissue microarray format. Cell densities and cell-to-cell distances were quantified. The interaction between G9-expressing tumor cell lines and T cells were also studied. Results: G9-expressing tumor cells were detected in all NPC cases and were significantly higher than normal tissue. Elevated G9 was associated with shorter overall survival (OS: 89% vs 70.5% at 7 years, p: 0.019). Incremental percentages of Tim3+ cells were shown in top 10% cases strongly positive for G9-expressing tumor cells. The number of Tim3+ cells was calculated at 15µm intervals from the nearest G9-expressing tumor cells, of which a significant difference of Tim3+ cells was observed at the 0-15µm distance from G9-expressing cell in cancerous compared to normal tissues. Epithelial short distances were associated with a unfavourable prognosis. Observed short distance were hypothesized to represent Tim3+ cells actively interacting with G9-expressing tumor cells. Accordingly, In vitro cocultured of G9-ovexpressing NPC cell lines induced Tim3 expression on T cells which suppressed the T-cell mediate cytotoxicity on tumor cells. Conclusions: Our findings indicate a specific preexisting profile of Tim3+ and G9-expressing tumor cells and demonstrated that Tim3+ cells were mainly found intratumorally within 15µm of a NPC cell. The relevance of Tim3+ and G9+ distances reflect a potential marker of their functional interaction. Our results could have important implications for clinical therapeutic strategies. Since high G9 expression have poorer OS, they would deserve a different therapeutic strategy. ©2021 American Society of Clinical Oncology, |
Description | Poster Session - Developmental Therapeutics—Immunotherapy - Abstract # 2629 |
Persistent Identifier | http://hdl.handle.net/10722/302355 |
ISSN | 2023 Impact Factor: 42.1 2023 SCImago Journal Rankings: 10.639 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Kwong, DLW | - |
dc.contributor.author | Kam, NW | - |
dc.contributor.author | Tse, WC | - |
dc.contributor.author | Lok, SHC | - |
dc.contributor.author | Tsao, GSW | - |
dc.contributor.author | Lee, VHF | - |
dc.date.accessioned | 2021-09-06T03:31:06Z | - |
dc.date.available | 2021-09-06T03:31:06Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | 2021 American Society of Clinical Oncology (ASCO) Annual Meeting, online meeting, 4-8 June 2021. In Journal of Clinical Oncology, 2021, v. 39 n. 15, Suppl., abstract no. 2629 | - |
dc.identifier.issn | 0732-183X | - |
dc.identifier.uri | http://hdl.handle.net/10722/302355 | - |
dc.description | Poster Session - Developmental Therapeutics—Immunotherapy - Abstract # 2629 | - |
dc.description.abstract | Background: The complex cell interactions within the tumor microenvironment (TME) have become a crucial point in cancer research. Yet, the cell interactions might not only depend on the frequency of immune cells, but also on the inter-individual distances as cells might interact via soluble factors and/or cell-cell contact. Accordingly, the mapping of TME has recently gained importance. The aim of this study is to investigate the alternations between galectin-9 (G9) and its natural immunosuppressive receptor, T cell immunoglobulin and mucin domain 3 (Tim3) in nasopharyngeal cancer (NPC). Methods: Using multiplexed quantitative immunofluorescence, we measured the levels of G9 and Tim3 in 95 NPC patients cancerous and 8 normal specimens in tissue microarray format. Cell densities and cell-to-cell distances were quantified. The interaction between G9-expressing tumor cell lines and T cells were also studied. Results: G9-expressing tumor cells were detected in all NPC cases and were significantly higher than normal tissue. Elevated G9 was associated with shorter overall survival (OS: 89% vs 70.5% at 7 years, p: 0.019). Incremental percentages of Tim3+ cells were shown in top 10% cases strongly positive for G9-expressing tumor cells. The number of Tim3+ cells was calculated at 15µm intervals from the nearest G9-expressing tumor cells, of which a significant difference of Tim3+ cells was observed at the 0-15µm distance from G9-expressing cell in cancerous compared to normal tissues. Epithelial short distances were associated with a unfavourable prognosis. Observed short distance were hypothesized to represent Tim3+ cells actively interacting with G9-expressing tumor cells. Accordingly, In vitro cocultured of G9-ovexpressing NPC cell lines induced Tim3 expression on T cells which suppressed the T-cell mediate cytotoxicity on tumor cells. Conclusions: Our findings indicate a specific preexisting profile of Tim3+ and G9-expressing tumor cells and demonstrated that Tim3+ cells were mainly found intratumorally within 15µm of a NPC cell. The relevance of Tim3+ and G9+ distances reflect a potential marker of their functional interaction. Our results could have important implications for clinical therapeutic strategies. Since high G9 expression have poorer OS, they would deserve a different therapeutic strategy. ©2021 American Society of Clinical Oncology, | - |
dc.language | eng | - |
dc.publisher | American Society of Clinical Oncology. The Journal's web site is located at http://www.jco.org/ | - |
dc.relation.ispartof | Journal of Clinical Oncology | - |
dc.relation.ispartof | 2021 American Society of Clinical Oncology (ASCO) Annual Meeting | - |
dc.title | The Tim3-galectin-9 interactions in the tumor microenvironment of nasopharyngeal cancer. | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Kwong, DLW: dlwkwong@hku.hk | - |
dc.identifier.email | Kam, NW: nwkam@hku.hk | - |
dc.identifier.email | Lok, SHC: cadenlok@hku.hk | - |
dc.identifier.email | Tsao, GSW: gswtsao@hku.hk | - |
dc.identifier.email | Lee, VHF: vhflee@hku.hk | - |
dc.identifier.authority | Kwong, DLW=rp00414 | - |
dc.identifier.authority | Tsao, GSW=rp00399 | - |
dc.identifier.authority | Lee, VHF=rp00264 | - |
dc.description.nature | abstract | - |
dc.identifier.doi | 10.1200/JCO.2021.39.15_suppl.2629 | - |
dc.identifier.hkuros | 324653 | - |
dc.identifier.volume | 39 | - |
dc.identifier.issue | 15, Suppl. | - |
dc.identifier.spage | abstract no. 2629 | - |
dc.identifier.epage | abstract no. 2629 | - |
dc.identifier.isi | WOS:000708120601150 | - |
dc.publisher.place | United States | - |