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Conference Paper: The Tim3-galectin-9 interactions in the tumor microenvironment of nasopharyngeal cancer.

TitleThe Tim3-galectin-9 interactions in the tumor microenvironment of nasopharyngeal cancer.
Authors
Issue Date2021
PublisherAmerican Society of Clinical Oncology. The Journal's web site is located at http://www.jco.org/
Citation
2021 American Society of Clinical Oncology (ASCO) Annual Meeting, online meeting, 4-8 June 2021. In Journal of Clinical Oncology, 2021, v. 39 n. 15, Suppl., abstract no. 2629 How to Cite?
AbstractBackground: The complex cell interactions within the tumor microenvironment (TME) have become a crucial point in cancer research. Yet, the cell interactions might not only depend on the frequency of immune cells, but also on the inter-individual distances as cells might interact via soluble factors and/or cell-cell contact. Accordingly, the mapping of TME has recently gained importance. The aim of this study is to investigate the alternations between galectin-9 (G9) and its natural immunosuppressive receptor, T cell immunoglobulin and mucin domain 3 (Tim3) in nasopharyngeal cancer (NPC). Methods: Using multiplexed quantitative immunofluorescence, we measured the levels of G9 and Tim3 in 95 NPC patients cancerous and 8 normal specimens in tissue microarray format. Cell densities and cell-to-cell distances were quantified. The interaction between G9-expressing tumor cell lines and T cells were also studied. Results: G9-expressing tumor cells were detected in all NPC cases and were significantly higher than normal tissue. Elevated G9 was associated with shorter overall survival (OS: 89% vs 70.5% at 7 years, p: 0.019). Incremental percentages of Tim3+ cells were shown in top 10% cases strongly positive for G9-expressing tumor cells. The number of Tim3+ cells was calculated at 15µm intervals from the nearest G9-expressing tumor cells, of which a significant difference of Tim3+ cells was observed at the 0-15µm distance from G9-expressing cell in cancerous compared to normal tissues. Epithelial short distances were associated with a unfavourable prognosis. Observed short distance were hypothesized to represent Tim3+ cells actively interacting with G9-expressing tumor cells. Accordingly, In vitro cocultured of G9-ovexpressing NPC cell lines induced Tim3 expression on T cells which suppressed the T-cell mediate cytotoxicity on tumor cells. Conclusions: Our findings indicate a specific preexisting profile of Tim3+ and G9-expressing tumor cells and demonstrated that Tim3+ cells were mainly found intratumorally within 15µm of a NPC cell. The relevance of Tim3+ and G9+ distances reflect a potential marker of their functional interaction. Our results could have important implications for clinical therapeutic strategies. Since high G9 expression have poorer OS, they would deserve a different therapeutic strategy. ©2021 American Society of Clinical Oncology,
DescriptionPoster Session - Developmental Therapeutics—Immunotherapy - Abstract # 2629
Persistent Identifierhttp://hdl.handle.net/10722/302355
ISSN
2023 Impact Factor: 42.1
2023 SCImago Journal Rankings: 10.639
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwong, DLW-
dc.contributor.authorKam, NW-
dc.contributor.authorTse, WC-
dc.contributor.authorLok, SHC-
dc.contributor.authorTsao, GSW-
dc.contributor.authorLee, VHF-
dc.date.accessioned2021-09-06T03:31:06Z-
dc.date.available2021-09-06T03:31:06Z-
dc.date.issued2021-
dc.identifier.citation2021 American Society of Clinical Oncology (ASCO) Annual Meeting, online meeting, 4-8 June 2021. In Journal of Clinical Oncology, 2021, v. 39 n. 15, Suppl., abstract no. 2629-
dc.identifier.issn0732-183X-
dc.identifier.urihttp://hdl.handle.net/10722/302355-
dc.descriptionPoster Session - Developmental Therapeutics—Immunotherapy - Abstract # 2629-
dc.description.abstractBackground: The complex cell interactions within the tumor microenvironment (TME) have become a crucial point in cancer research. Yet, the cell interactions might not only depend on the frequency of immune cells, but also on the inter-individual distances as cells might interact via soluble factors and/or cell-cell contact. Accordingly, the mapping of TME has recently gained importance. The aim of this study is to investigate the alternations between galectin-9 (G9) and its natural immunosuppressive receptor, T cell immunoglobulin and mucin domain 3 (Tim3) in nasopharyngeal cancer (NPC). Methods: Using multiplexed quantitative immunofluorescence, we measured the levels of G9 and Tim3 in 95 NPC patients cancerous and 8 normal specimens in tissue microarray format. Cell densities and cell-to-cell distances were quantified. The interaction between G9-expressing tumor cell lines and T cells were also studied. Results: G9-expressing tumor cells were detected in all NPC cases and were significantly higher than normal tissue. Elevated G9 was associated with shorter overall survival (OS: 89% vs 70.5% at 7 years, p: 0.019). Incremental percentages of Tim3+ cells were shown in top 10% cases strongly positive for G9-expressing tumor cells. The number of Tim3+ cells was calculated at 15µm intervals from the nearest G9-expressing tumor cells, of which a significant difference of Tim3+ cells was observed at the 0-15µm distance from G9-expressing cell in cancerous compared to normal tissues. Epithelial short distances were associated with a unfavourable prognosis. Observed short distance were hypothesized to represent Tim3+ cells actively interacting with G9-expressing tumor cells. Accordingly, In vitro cocultured of G9-ovexpressing NPC cell lines induced Tim3 expression on T cells which suppressed the T-cell mediate cytotoxicity on tumor cells. Conclusions: Our findings indicate a specific preexisting profile of Tim3+ and G9-expressing tumor cells and demonstrated that Tim3+ cells were mainly found intratumorally within 15µm of a NPC cell. The relevance of Tim3+ and G9+ distances reflect a potential marker of their functional interaction. Our results could have important implications for clinical therapeutic strategies. Since high G9 expression have poorer OS, they would deserve a different therapeutic strategy. ©2021 American Society of Clinical Oncology,-
dc.languageeng-
dc.publisherAmerican Society of Clinical Oncology. The Journal's web site is located at http://www.jco.org/-
dc.relation.ispartofJournal of Clinical Oncology-
dc.relation.ispartof2021 American Society of Clinical Oncology (ASCO) Annual Meeting-
dc.titleThe Tim3-galectin-9 interactions in the tumor microenvironment of nasopharyngeal cancer.-
dc.typeConference_Paper-
dc.identifier.emailKwong, DLW: dlwkwong@hku.hk-
dc.identifier.emailKam, NW: nwkam@hku.hk-
dc.identifier.emailLok, SHC: cadenlok@hku.hk-
dc.identifier.emailTsao, GSW: gswtsao@hku.hk-
dc.identifier.emailLee, VHF: vhflee@hku.hk-
dc.identifier.authorityKwong, DLW=rp00414-
dc.identifier.authorityTsao, GSW=rp00399-
dc.identifier.authorityLee, VHF=rp00264-
dc.description.natureabstract-
dc.identifier.doi10.1200/JCO.2021.39.15_suppl.2629-
dc.identifier.hkuros324653-
dc.identifier.volume39-
dc.identifier.issue15, Suppl.-
dc.identifier.spageabstract no. 2629-
dc.identifier.epageabstract no. 2629-
dc.identifier.isiWOS:000708120601150-
dc.publisher.placeUnited States-

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