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Article: Maturation of Neural Cells Leads to Enhanced Axon-Extracellular Matrix Adhesion and Altered Injury Response

TitleMaturation of Neural Cells Leads to Enhanced Axon-Extracellular Matrix Adhesion and Altered Injury Response
Authors
Keywordsneuron adhesion
axon retraction
traumatic injury
neuron mechanics
cell adhesion
Issue Date2021
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/bioengineering_and_biotechnology
Citation
Frontiers in Bioengineering and Biotechnology, 2021, v. 8, p. article no. 621777 How to Cite?
AbstractAlthough it is known that stronger cell-extracellular matrix interactions will be developed as neurons mature, how such change influences their response against traumatic injury remains largely unknown. In this report, by transecting axons with a sharp atomic force microscope tip, we showed that the injury-induced retracting motion of axon can be temporarily arrested by tight NCAM (neural cell adhesion molecule) mediated adhesion patches, leading to a retraction curve decorated with sudden bursts. Interestingly, although the size of adhesion clusters (~0.5–1 μm) was found to be more or less the same in mature and immature neurons (after 7- and 3-days of culturing, respectively), the areal density of such clusters is three times higher in mature axons resulting in a much reduced retraction in response to injury. A physical model was also adopted to explain the observed retraction trajectories which suggested that apparent adhesion energy between axon and the substrate increases from ~0.12 to 0.39 mJ/m2 as neural cell matures, in good agreement with our experiments.
Descriptioneid_2-s2.0-85099728295
Persistent Identifierhttp://hdl.handle.net/10722/304549
ISSN
2023 Impact Factor: 4.3
2023 SCImago Journal Rankings: 0.893
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShao, X-
dc.contributor.authorSOERENSEN, MH-
dc.contributor.authorFang, C-
dc.contributor.authorChang, RCC-
dc.contributor.authorChu, Z-
dc.contributor.authorLin, Y-
dc.date.accessioned2021-09-23T09:01:38Z-
dc.date.available2021-09-23T09:01:38Z-
dc.date.issued2021-
dc.identifier.citationFrontiers in Bioengineering and Biotechnology, 2021, v. 8, p. article no. 621777-
dc.identifier.issn2296-4185-
dc.identifier.urihttp://hdl.handle.net/10722/304549-
dc.descriptioneid_2-s2.0-85099728295-
dc.description.abstractAlthough it is known that stronger cell-extracellular matrix interactions will be developed as neurons mature, how such change influences their response against traumatic injury remains largely unknown. In this report, by transecting axons with a sharp atomic force microscope tip, we showed that the injury-induced retracting motion of axon can be temporarily arrested by tight NCAM (neural cell adhesion molecule) mediated adhesion patches, leading to a retraction curve decorated with sudden bursts. Interestingly, although the size of adhesion clusters (~0.5–1 μm) was found to be more or less the same in mature and immature neurons (after 7- and 3-days of culturing, respectively), the areal density of such clusters is three times higher in mature axons resulting in a much reduced retraction in response to injury. A physical model was also adopted to explain the observed retraction trajectories which suggested that apparent adhesion energy between axon and the substrate increases from ~0.12 to 0.39 mJ/m2 as neural cell matures, in good agreement with our experiments.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/bioengineering_and_biotechnology-
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectneuron adhesion-
dc.subjectaxon retraction-
dc.subjecttraumatic injury-
dc.subjectneuron mechanics-
dc.subjectcell adhesion-
dc.titleMaturation of Neural Cells Leads to Enhanced Axon-Extracellular Matrix Adhesion and Altered Injury Response-
dc.typeArticle-
dc.identifier.emailShao, X: shaoxy12@hku.hk-
dc.identifier.emailFang, C: fangchao@hku.hk-
dc.identifier.emailChang, RCC: rccchang@hku.hk-
dc.identifier.emailChu, Z: zqchu@eee.hku.hk-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.authorityChang, RCC=rp00470-
dc.identifier.authorityChu, Z=rp02472-
dc.identifier.authorityLin, Y=rp00080-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fbioe.2020.621777-
dc.identifier.pmid33490057-
dc.identifier.pmcidPMC7815929-
dc.identifier.scopuseid_2-s2.0-85099728295-
dc.identifier.hkuros325045-
dc.identifier.volume8-
dc.identifier.spagearticle no. 621777-
dc.identifier.epagearticle no. 621777-
dc.identifier.isiWOS:000608787500001-
dc.publisher.placeSwitzerland-

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