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Article: Axially resolved volumetric two-photon microscopy with an extended field of view using depth localization under mirrored Airy beams

TitleAxially resolved volumetric two-photon microscopy with an extended field of view using depth localization under mirrored Airy beams
Authors
Issue Date2020
PublisherOptical Society of America: Open Access Journals. The Journal's web site is located at http://www.opticsexpress.org
Citation
Optics Express, 2020, v. 28, p. 39563-39573 How to Cite?
AbstractIt is a great challenge in two-photon microscopy (2PM) to have a high volumetric imaging speed without sacrificing the spatial and temporal resolution in three dimensions (3D). The structure in 2PM images could be reconstructed with better spatial and temporal resolution by the proper choice of the data processing algorithm. Here, we propose a method to reconstruct 3D volume from 2D projections imaged by mirrored Airy beams. We verified that our approach can achieve high accuracy in 3D localization over a large axial range and is applicable to continuous and dense sample. The effective field of view after reconstruction is expanded. It is a promising technique for rapid volumetric 2PM with axial localization at high resolution. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Persistent Identifierhttp://hdl.handle.net/10722/304550
ISSN
2023 Impact Factor: 3.2
2023 SCImago Journal Rankings: 0.998
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCHAN, RKY-
dc.contributor.authorHE, H-
dc.contributor.authorRen, YX-
dc.contributor.authorLai, CSW-
dc.contributor.authorLam, EY-
dc.contributor.authorWong, KKY-
dc.date.accessioned2021-09-23T09:01:38Z-
dc.date.available2021-09-23T09:01:38Z-
dc.date.issued2020-
dc.identifier.citationOptics Express, 2020, v. 28, p. 39563-39573-
dc.identifier.issn1094-4087-
dc.identifier.urihttp://hdl.handle.net/10722/304550-
dc.description.abstractIt is a great challenge in two-photon microscopy (2PM) to have a high volumetric imaging speed without sacrificing the spatial and temporal resolution in three dimensions (3D). The structure in 2PM images could be reconstructed with better spatial and temporal resolution by the proper choice of the data processing algorithm. Here, we propose a method to reconstruct 3D volume from 2D projections imaged by mirrored Airy beams. We verified that our approach can achieve high accuracy in 3D localization over a large axial range and is applicable to continuous and dense sample. The effective field of view after reconstruction is expanded. It is a promising technique for rapid volumetric 2PM with axial localization at high resolution. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement-
dc.languageeng-
dc.publisherOptical Society of America: Open Access Journals. The Journal's web site is located at http://www.opticsexpress.org-
dc.relation.ispartofOptics Express-
dc.rightsOptics Express. Copyright © Optical Society of America: Open Access Journals.-
dc.rights© 2020 [Optical Society of America]. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleAxially resolved volumetric two-photon microscopy with an extended field of view using depth localization under mirrored Airy beams-
dc.typeArticle-
dc.identifier.emailLai, CSW: coraswl@hku.hk-
dc.identifier.emailLam, EY: elam@eee.hku.hk-
dc.identifier.emailWong, KKY: kywong@eee.hku.hk-
dc.identifier.authorityLai, CSW=rp01895-
dc.identifier.authorityLam, EY=rp00131-
dc.identifier.authorityWong, KKY=rp00189-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1364/OE.412453-
dc.identifier.pmid33379502-
dc.identifier.scopuseid_2-s2.0-85097595144-
dc.identifier.hkuros325051-
dc.identifier.volume28-
dc.identifier.spage39563-
dc.identifier.epage39573-
dc.identifier.isiWOS:000600667300088-
dc.publisher.placeUnited States-

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