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Article: Background-Free Volumetric Two-Photon Microscopy by Side-Lobes-Cancelled Bessel Beam

TitleBackground-Free Volumetric Two-Photon Microscopy by Side-Lobes-Cancelled Bessel Beam
Authors
KeywordsBessel beam
side lobes
two-photon microscopy
volumetric imaging
contrast enhancement
Issue Date2021
PublisherInstitute of Electrical and Electronics Engineers. The Journal's web site is located at http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=2944
Citation
IEEE Journal of Selected Topics in Quantum Electronics, 2021, v. 27 n. 4, article no. 6801307 How to Cite?
AbstractWe have demonstrated the background-free volumetric two-photon microscopy by cancelling the side lobes of the fundamental 0th-order Bessel beam. The cancellation is based on the fact that the lateral ring patterns of the 3rd-order Bessel beam are well-matched with the side lobes of the 0th-order Bessel beam, so that the image obtained by the 3rd-order Bessel beam is capable to be subtracted as the background of the 0th-order-Bessel-beam-scanned image. We investigated the intensity of the imaging background introduced by the Bessel side lobes under different focusing conditions both in simulations and experiments. The performance of the side-lobes cancellation is showcased on a 50-μm-thick mouse brain slice, and the contrast ratio is enhanced by 2.7 dB for the neurons. This background cancellation technique provides a simple and versatile tool for enhancing the contrast ratio in the two-photon volumetric imaging, especially critical in high-NA cases when a better lateral resolution is pursued.
Persistent Identifierhttp://hdl.handle.net/10722/304552
ISSN
2023 Impact Factor: 4.3
2023 SCImago Journal Rankings: 1.283
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHE, H-
dc.contributor.authorRen, YX-
dc.contributor.authorCHAN, RKY-
dc.contributor.authorSO, WL-
dc.contributor.authorFOK, HK-
dc.contributor.authorLai, CSW-
dc.contributor.authorTsia, KK-
dc.contributor.authorWong, KKY-
dc.date.accessioned2021-09-23T09:01:40Z-
dc.date.available2021-09-23T09:01:40Z-
dc.date.issued2021-
dc.identifier.citationIEEE Journal of Selected Topics in Quantum Electronics, 2021, v. 27 n. 4, article no. 6801307-
dc.identifier.issn1077-260X-
dc.identifier.urihttp://hdl.handle.net/10722/304552-
dc.description.abstractWe have demonstrated the background-free volumetric two-photon microscopy by cancelling the side lobes of the fundamental 0th-order Bessel beam. The cancellation is based on the fact that the lateral ring patterns of the 3rd-order Bessel beam are well-matched with the side lobes of the 0th-order Bessel beam, so that the image obtained by the 3rd-order Bessel beam is capable to be subtracted as the background of the 0th-order-Bessel-beam-scanned image. We investigated the intensity of the imaging background introduced by the Bessel side lobes under different focusing conditions both in simulations and experiments. The performance of the side-lobes cancellation is showcased on a 50-μm-thick mouse brain slice, and the contrast ratio is enhanced by 2.7 dB for the neurons. This background cancellation technique provides a simple and versatile tool for enhancing the contrast ratio in the two-photon volumetric imaging, especially critical in high-NA cases when a better lateral resolution is pursued.-
dc.languageeng-
dc.publisherInstitute of Electrical and Electronics Engineers. The Journal's web site is located at http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=2944-
dc.relation.ispartofIEEE Journal of Selected Topics in Quantum Electronics-
dc.rightsIEEE Journal of Selected Topics in Quantum Electronics. Copyright © Institute of Electrical and Electronics Engineers.-
dc.rights©2021 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.-
dc.subjectBessel beam-
dc.subjectside lobes-
dc.subjecttwo-photon microscopy-
dc.subjectvolumetric imaging-
dc.subjectcontrast enhancement-
dc.titleBackground-Free Volumetric Two-Photon Microscopy by Side-Lobes-Cancelled Bessel Beam-
dc.typeArticle-
dc.identifier.emailLai, CSW: coraswl@hku.hk-
dc.identifier.emailTsia, KK: tsia@hku.hk-
dc.identifier.emailWong, KKY: kywong@eee.hku.hk-
dc.identifier.authorityLai, CSW=rp01895-
dc.identifier.authorityTsia, KK=rp01389-
dc.identifier.authorityWong, KKY=rp00189-
dc.description.naturepostprint-
dc.identifier.doi10.1109/JSTQE.2021.3054892-
dc.identifier.scopuseid_2-s2.0-85100456087-
dc.identifier.hkuros325283-
dc.identifier.volume27-
dc.identifier.issue4-
dc.identifier.spagearticle no. 6801307-
dc.identifier.epagearticle no. 6801307-
dc.identifier.isiWOS:000620783700002-
dc.publisher.placeUnited States-

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